Background and AimsHepatobiliary phase (HBP) Gd‐EOB‐DTPA‐enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd‐EOB‐DTPA‐enhanced MRI.MethodsBy means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA‐HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA‐HCC (n = 1) with Gd‐EOB‐DTPA MR images.ResultsAll FNH showed a preserved expression of hepatocytes transporters. Beta‐catenin‐activated HCA (at highest risk of malignant transformation) and HCA‐HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P P ConclusionsOATPB1/B3 and MRP3 IHC and signal intensity on HBP Gd‐EOB‐DTPA‐enhanced MRI can help to stratify HCA according to their risk of malignant transformation.
Background and AimsHepatobiliary phase (HBP) Gd‐EOB‐DTPA‐enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd‐EOB‐DTPA‐enhanced MRI.MethodsBy means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA‐HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA‐HCC (n = 1) with Gd‐EOB‐DTPA MR images.ResultsAll FNH showed a preserved expression of hepatocytes transporters. Beta‐catenin‐activated HCA (at highest risk of malignant transformation) and HCA‐HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P P ConclusionsOATPB1/B3 and MRP3 IHC and signal intensity on HBP Gd‐EOB‐DTPA‐enhanced MRI can help to stratify HCA according to their risk of malignant transformation.
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