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Cytosolic-free calcium in smooth-muscle and endothelial cells in an intact arterial wall from rat mesenteric artery in vitro

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Title: Cytosolic-free calcium in smooth-muscle and endothelial cells in an intact arterial wall from rat mesenteric artery in vitro
Authors: Oishi, Hirotaka, Budel, Stéphane, Schuster, A., Stergiopulos, N., Meister, J.J., Beny, Jean-Louis
Source: Cell Calcium, Vol. 30, No 4 (2001) pp. 261-267
Publisher Information: Elsevier BV, 2001.
Publication Year: 2001
Subject Terms: Cytosol/metabolism, Male, 0301 basic medicine, Fluorescent Dyes/analysis, Calcium/metabolism, Vasodilator Agents, Phenylephrine/pharmacology, Adrenergic alpha-Agonists/pharmacology, In Vitro Techniques, Rats, Sprague-Dawley, Phenylephrine, 03 medical and health sciences, Cytosol, ddc:590, Vascular/drug effects/metabolism, Imidazoles/analysis, Animals, Endothelium, Vasodilator Agents/pharmacology, Aniline Compounds/analysis, Benzofurans, Fluorescent Dyes, 0303 health sciences, Acetylcholine/pharmacology, Aniline Compounds, Smooth/drug effects/metabolism, Imidazoles, Muscle, Smooth, Acetylcholine, Mesenteric Arteries, Rats, Potassium/pharmacology, Xanthenes, Benzofurans/analysis, Xanthenes/analysis, Potassium, Muscle, Calcium, Sprague-Dawley, Endothelium, Vascular, Adrenergic alpha-Agonists
Description: The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examined in an intact arterial wall in vitro. Strips of the main branch of rat mesenteric artery were used. Cytosolic-free calcium concentration [Ca2+]i was estimated by determining the fluorescence ratio of the two calcium probes, Fluo-4 and Fura red. The emitted fluorescence of both probes was measured with a confocal microscope. We showed that potassium and phenylephrine, which increase the cytosolic -free calcium concentration of the smooth-muscle cells, also indirectly influence the calcium concentration in the endothelial cells. By simultaneously determining [Ca2+]i in the endothelial and the smooth-muscle cells of an arterial strip, we observed that when calcium increases in the endothelial cells in response to acetylcholine, it slightly decreases in the smooth-muscle cells. We conclude that the regulation of [Ca2+]i in the arterial endothelial cell, depends according to the stimuli either upon the endothelial cells themselves, or upon the coupled smooth-muscle cells.
Document Type: Article
File Description: application/pdf
Language: English
ISSN: 0143-4160
DOI: 10.1054/ceca.2001.0233
Access URL: https://pubmed.ncbi.nlm.nih.gov/11587550
https://infoscience.epfl.ch/record/147791
https://www.sciencedirect.com/science/article/pii/S0143416001902337
https://archive-ouverte.unige.ch/unige:85060
https://archive-ouverte.unige.ch/unige:85060
Rights: Elsevier TDM
Accession Number: edsair.doi.dedup.....b9a12c10d1ac677bd8de592c7af9ec09
Database: OpenAIRE
Description
Abstract:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examined in an intact arterial wall in vitro. Strips of the main branch of rat mesenteric artery were used. Cytosolic-free calcium concentration [Ca2+]i was estimated by determining the fluorescence ratio of the two calcium probes, Fluo-4 and Fura red. The emitted fluorescence of both probes was measured with a confocal microscope. We showed that potassium and phenylephrine, which increase the cytosolic -free calcium concentration of the smooth-muscle cells, also indirectly influence the calcium concentration in the endothelial cells. By simultaneously determining [Ca2+]i in the endothelial and the smooth-muscle cells of an arterial strip, we observed that when calcium increases in the endothelial cells in response to acetylcholine, it slightly decreases in the smooth-muscle cells. We conclude that the regulation of [Ca2+]i in the arterial endothelial cell, depends according to the stimuli either upon the endothelial cells themselves, or upon the coupled smooth-muscle cells.
ISSN:01434160
DOI:10.1054/ceca.2001.0233