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Optical trapping with nanostructured optical fibers and motility analysis of Pseudomonas aeruginosa

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Bibliographic Details
Title: Optical trapping with nanostructured optical fibers and motility analysis of Pseudomonas aeruginosa
Authors: Eric Faudry, Jochen Fick
Contributors: Faudry, Eric
Source: Eur Biophys J
Publisher Information: Springer Science and Business Media LLC, 2025.
Publication Year: 2025
Subject Terms: [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Original Article, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Description: The study of bacteria swimming behavior or their interaction with other bacteria or cells requires an efficient and flexible tool for bacteria manipulation. Optical tweezers have been shown to be perfectly adapted for this task. Here we report optical trapping of pathogen Pseudomonas aeruginosa bacteria using optical fiber tweezers with dedicated nanostructured optical fibers. Well-aligned straight chains of up to ten bacteria were observed with optical fiber tips, whereas contactless trapping was realized at distances of 100 and 45 µm for Fresnel lens fibers and TIROFs, respectively. Very efficient trapping at laser powers as low as 3.7 mW was achieved. The bacteria vitality is an important parameter in trapping experiments. Mean square displacement and speed autocorrelation methods were applied to obtain a vitality measure and to classify the free bacteria trajectories into free floating, running, and run-wrap-run categories. The high frame rates of our observation videos allow us to reveal a relation between bacteria speed and bacteria orientation oscillations.
Document Type: Article
Other literature type
File Description: application/pdf
Language: English
ISSN: 1432-1017
0175-7571
DOI: 10.1007/s00249-025-01775-7
Rights: CC BY
Accession Number: edsair.doi.dedup.....b6f0ff944dd5eee2c359a3f57220624f
Database: OpenAIRE
Description
Abstract:The study of bacteria swimming behavior or their interaction with other bacteria or cells requires an efficient and flexible tool for bacteria manipulation. Optical tweezers have been shown to be perfectly adapted for this task. Here we report optical trapping of pathogen Pseudomonas aeruginosa bacteria using optical fiber tweezers with dedicated nanostructured optical fibers. Well-aligned straight chains of up to ten bacteria were observed with optical fiber tips, whereas contactless trapping was realized at distances of 100 and 45 µm for Fresnel lens fibers and TIROFs, respectively. Very efficient trapping at laser powers as low as 3.7 mW was achieved. The bacteria vitality is an important parameter in trapping experiments. Mean square displacement and speed autocorrelation methods were applied to obtain a vitality measure and to classify the free bacteria trajectories into free floating, running, and run-wrap-run categories. The high frame rates of our observation videos allow us to reveal a relation between bacteria speed and bacteria orientation oscillations.
ISSN:14321017
01757571
DOI:10.1007/s00249-025-01775-7