Changes in Genetic Diversity from Field to Laboratory During Colonization of Anopheles darlingi Root (Diptera: Culicidae)
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| Název: | Changes in Genetic Diversity from Field to Laboratory During Colonization of Anopheles darlingi Root (Diptera: Culicidae) |
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| Autoři: | Lainhart, William, Bickersmith, Sara A., Moreno, Marta, Rios, Carlos Tong, Vinetz, Joseph Michael, Conn, Jan E. |
| Zdroj: | The American Society of Tropical Medicine and Hygiene. 93:998-1001 |
| Informace o vydavateli: | American Society of Tropical Medicine and Hygiene, 2015. |
| Rok vydání: | 2015 |
| Témata: | Microsatellite Repeats/genetics, Genetic Variation, Anopheles/genetics, 16. Peace & justice, Insect Vectors, Malaria, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, Anopheles, Malaria/transmission, Animals, Inbreeding, Laboratories, Insect Vectors/genetics, Microsatellite Repeats |
| Popis: | The process of colonizing any arthropod species, including vector mosquitoes, necessarily involves adaptation to laboratory conditions. The adaptation and evolution of colonized mosquito populations needs consideration when such colonies are used as representative models for pathogen transmission dynamics. A recently established colony of Anopheles darlingi, the primary malaria vector in Amazonian South America, was tested for genetic diversity and bottleneck after 21 generations, using microsatellites. As expected, laboratory An. darlingi had fewer private and rare alleles (frequency < 0.05), decreased observed heterozygosity, and more common alleles (frequency > 0.50), but no significant evidence of a bottleneck, decrease in total alleles, or increase in inbreeding compared with field specimens (founder population). Low-moderate differentiation between field and laboratory populations was detected. With these findings, and the documented inherent differences between laboratory and field populations, results of pathogen transmission studies using this An. darlingi colony need to be interpreted cautiously. |
| Druh dokumentu: | Article |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.15-0336 |
| Přístupová URL adresa: | https://europepmc.org/articles/pmc4703261?pdf=render https://pubmed.ncbi.nlm.nih.gov/26283742 http://www.ajtmh.org/content/journals/10.4269/ajtmh.15-0336 https://europepmc.org/article/PMC/PMC4703261 https://www.ajtmh.org/view/journals/tpmd/93/5/article-p998.xml https://www.ncbi.nlm.nih.gov/pubmed/26283742 https://hdl.handle.net/20.500.12866/5467 https://doi.org/10.4269/ajtmh.15-0336 |
| Přístupové číslo: | edsair.doi.dedup.....b4b4c2f01f697382ba14e102b41d8301 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The process of colonizing any arthropod species, including vector mosquitoes, necessarily involves adaptation to laboratory conditions. The adaptation and evolution of colonized mosquito populations needs consideration when such colonies are used as representative models for pathogen transmission dynamics. A recently established colony of Anopheles darlingi, the primary malaria vector in Amazonian South America, was tested for genetic diversity and bottleneck after 21 generations, using microsatellites. As expected, laboratory An. darlingi had fewer private and rare alleles (frequency < 0.05), decreased observed heterozygosity, and more common alleles (frequency > 0.50), but no significant evidence of a bottleneck, decrease in total alleles, or increase in inbreeding compared with field specimens (founder population). Low-moderate differentiation between field and laboratory populations was detected. With these findings, and the documented inherent differences between laboratory and field populations, results of pathogen transmission studies using this An. darlingi colony need to be interpreted cautiously. |
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| ISSN: | 14761645 00029637 |
| DOI: | 10.4269/ajtmh.15-0336 |