Genome-wide association for metabolic clusters in early-lactation Holstein dairy cows
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| Název: | Genome-wide association for metabolic clusters in early-lactation Holstein dairy cows |
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| Autoři: | Atashi, Hadi, Salavati, Mazdak, De Koster, J., Crowe, M. A., Opsomer, G., The GplusE consortium, Hostens, M. |
| Zdroj: | Atashi, H, Salavati, M, De Koster, J, Crowe, M A, Opsomer, G, The GplusE consortium & Hostens, M 2020, ' Genome-wide association for metabolic clusters in early-lactation Holstein dairy cows ', Journal of Dairy Science . https://doi.org/10.3168/jds.2019-17369 Atashi, H, Salavati, M, De Koster, J, Crowe, M, Opsomer, G, GplusE Consortium & Hostens, M 2020, 'Genome-wide association for metabolic clusters in early-lactation Holstein dairy cows', Journal of Dairy Science, vol. 103, no. 7, pp. 6392-6406. https://doi.org/10.3168/jds.2019-17369 |
| Informace o vydavateli: | American Dairy Science Association, 2020. |
| Rok vydání: | 2020 |
| Témata: | Blood Glucose, 0301 basic medicine, CATTLE, Fatty Acids, Nonesterified, FEED-EFFICIENCY, 03 medical and health sciences, Pregnancy, Milk/chemistry, Genetics, Animals, Cluster Analysis, Lactation, Cattle/metabolism, Animal production & animal husbandry, transition period, 2. Zero hunger, QUANTITATIVE TRAIT LOCI, 0303 health sciences, HEALTH TRAITS, GENETIC-PARAMETERS, genome-wide association study, Blood Glucose/metabolism, 3-Hydroxybutyric Acid, Gene Expression Regulation/physiology, ANKYRIN-1 GENE, dairy cow, metabolic adaptation, Milk Proteins, NEGATIVE-ENERGY BALANCE, Fatty Acids, Nonesterified/blood, Life sciences, Productions animales & zootechnie, Milk, Gene Expression Regulation, 3-Hydroxybutyric Acid/blood, DISEASES, Case-Control Studies, ADAPTATIONS, Sciences du vivant, Animal Science and Zoology, Cattle, Female, MILK-PRODUCTION, Milk Proteins/analysis, Lactation/physiology, Food Science, Genome-Wide Association Study |
| Popis: | The aim of this study was to detect the genomic region or regions associated with metabolic clusters in early-lactation Holstein cows. This study was carried out in 2 experiments. In experiment I, which was carried out on 105 multiparous Holstein cows, animals were classified through k-means clustering on log-transformed and standardized concentrations of blood glucose, insulin-like growth factor I, free fatty acids, and β-hydroxybutyrate at 14 and 35 d in milk (DIM), into metabolic clusters, either balanced (BAL) or other (OTR). Forty percent of the animals were categorized in the BAL group, and the remainder were categorized as OTR. The cows were genotyped for a total of 777,962 SNP. A genome-wide association study was performed, using a case-control approach through the GEMMA software, accounting for population structure. We found 8 SNP (BTA11, BTA23, and BTAX) associated with the predicted metabolic clusters. In experiment II, carried out on 4,267 second-parity Holstein cows, milk samples collected starting from the first week until 50 DIM were used to determine Fourier-transform mid-infrared (FT-MIR) spectra and subsequently to classify the animals into the same metabolic clusters (BAL vs. OTR). Twenty-eight percent of the animals were categorized in the BAL group, and the remainder were classified in the OTR category. Although daily milk yield was lower in BAL cows, we found no difference in daily fat- and protein-corrected milk yield in cows from the BAL metabolic cluster compared with those in the OTR metabolic cluster. In the next step, a single-step genomic BLUP was used to identify the genomic region(s) associated with the predicted metabolic clusters. The results revealed that prediction of metabolic clusters is a highly polygenic trait regulated by many small-sized effects. The region of 36,258 to 36,295 kb on BTA27 was the highly associated region for the predicted metabolic clusters, with the closest genes to this region (ANK1 and miR-486) being related to hematopoiesis, erythropoiesis, and mammary gland development. The heritability for metabolic clustering was 0.17 (SD 0.03), indicating that the use of FT-MIR spectra in milk to predict metabolic clusters in early-lactation across a large number of cows has satisfactory potential to be included in genetic selection programs for modern dairy cows. |
| Druh dokumentu: | Article |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2019-17369 |
| Přístupová URL adresa: | http://www.journalofdairyscience.org/article/S0022030220303015/pdf https://pubmed.ncbi.nlm.nih.gov/32331880 https://biblio.ugent.be/publication/8669484 https://pubmed.ncbi.nlm.nih.gov/32331880/ https://www.sciencedirect.com/science/article/abs/pii/S0022030220303015 https://www.sciencedirect.com/science/article/pii/S0022030220303015 https://www.ncbi.nlm.nih.gov/pubmed/32331880 https://www.pure.ed.ac.uk/ws/files/131320058/JDS.2019_17369.R2_Proof_hi.pdf https://hdl.handle.net/20.500.11820/fdd23dd0-b684-4ac1-b2fb-b18b3a06444d https://pure.au.dk/portal/en/publications/54f3e139-765e-4c99-ba5d-c741b0c6ccdd https://doi.org/10.3168/jds.2019-17369 |
| Rights: | Elsevier Non-Commercial |
| Přístupové číslo: | edsair.doi.dedup.....b44668a5f2a368800abe700043c58f33 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The aim of this study was to detect the genomic region or regions associated with metabolic clusters in early-lactation Holstein cows. This study was carried out in 2 experiments. In experiment I, which was carried out on 105 multiparous Holstein cows, animals were classified through k-means clustering on log-transformed and standardized concentrations of blood glucose, insulin-like growth factor I, free fatty acids, and β-hydroxybutyrate at 14 and 35 d in milk (DIM), into metabolic clusters, either balanced (BAL) or other (OTR). Forty percent of the animals were categorized in the BAL group, and the remainder were categorized as OTR. The cows were genotyped for a total of 777,962 SNP. A genome-wide association study was performed, using a case-control approach through the GEMMA software, accounting for population structure. We found 8 SNP (BTA11, BTA23, and BTAX) associated with the predicted metabolic clusters. In experiment II, carried out on 4,267 second-parity Holstein cows, milk samples collected starting from the first week until 50 DIM were used to determine Fourier-transform mid-infrared (FT-MIR) spectra and subsequently to classify the animals into the same metabolic clusters (BAL vs. OTR). Twenty-eight percent of the animals were categorized in the BAL group, and the remainder were classified in the OTR category. Although daily milk yield was lower in BAL cows, we found no difference in daily fat- and protein-corrected milk yield in cows from the BAL metabolic cluster compared with those in the OTR metabolic cluster. In the next step, a single-step genomic BLUP was used to identify the genomic region(s) associated with the predicted metabolic clusters. The results revealed that prediction of metabolic clusters is a highly polygenic trait regulated by many small-sized effects. The region of 36,258 to 36,295 kb on BTA27 was the highly associated region for the predicted metabolic clusters, with the closest genes to this region (ANK1 and miR-486) being related to hematopoiesis, erythropoiesis, and mammary gland development. The heritability for metabolic clustering was 0.17 (SD 0.03), indicating that the use of FT-MIR spectra in milk to predict metabolic clusters in early-lactation across a large number of cows has satisfactory potential to be included in genetic selection programs for modern dairy cows. |
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| ISSN: | 00220302 |
| DOI: | 10.3168/jds.2019-17369 |