Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development
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| Titel: | Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development |
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| Autoren: | R. Castilla, Toru Suzuki, Anthony C.F. Perry, María Isabel Arjona, Matthew D. VerMilyea, José Antonio Plaza, Maki Asami, Marta Duch, R. Gómez-Martínez, Núria Torras |
| Weitere Verfasser: | Universitat Politècnica de Catalunya. Departament de Mecànica de Fluids, Universitat Politècnica de Catalunya. LABSON - Laboratori de Sistemes Oleohidràulics i Pneumàtics |
| Quelle: | UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Duch, M, Torras, N, Asami, M, Suzuki, T, Arjona, M, Gómez-Martínez, R, VerMilyea, M, Castilla, R, Plaza, J A & Perry, A 2020, 'Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development', Nature Materials, vol. 19, no. 10, pp. 1114-1123. https://doi.org/10.1038/s41563-020-0685-9 |
| Verlagsinformationen: | Springer Science and Business Media LLC, 2020. |
| Publikationsjahr: | 2020 |
| Schlagwörter: | Male, 0301 basic medicine, name=General Chemistry, 0303 health sciences, Nanotecnologia, Embryonic Development, Embryo, Mammalian, name=General Materials Science, Biomechanical Phenomena, name=Condensed Matter Physics, Mice, 03 medical and health sciences, Biosensors, name=Mechanics of Materials, Nanotechnology, Animals, Enginyeria biomèdica::Biomecànica [Àrees temàtiques de la UPC], Female, Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomecànica, name=Mechanical Engineering, Sistemes nanoelectromecànics, Single-Cell Analysis, Nanoelectromechanical systems |
| Beschreibung: | Cells comprise mechanically active matter that governs their functionality, but intracellular mechanics are difficult to study directly and are poorly understood. However, injected nanodevices open up opportunities to analyse intracellular mechanobiology. Here, we identify a programme of forces and changes to the cytoplasmic mechanical properties required for mouse embryo development from fertilization to the first cell division. Injected, fully internalized nanodevices responded to sperm decondensation and recondensation, and subsequent device behaviour suggested a model for pronuclear convergence based on a gradient of effective cytoplasmic stiffness. The nanodevices reported reduced cytoplasmic mechanical activity during chromosome alignment and indicated that cytoplasmic stiffening occurred during embryo elongation, followed by rapid cytoplasmic softening during cytokinesis (cell division). Forces greater than those inside muscle cells were detected within embryos. These results suggest that intracellular forces are part of a concerted programme that is necessary for development at the origin of a new embryonic life. |
| Publikationsart: | Article |
| Dateibeschreibung: | application/pdf |
| Sprache: | English |
| ISSN: | 1476-4660 1476-1122 |
| DOI: | 10.1038/s41563-020-0685-9 |
| Zugangs-URL: | https://upcommons.upc.edu/bitstream/2117/330111/4/Duch%2c%20M.%20Tracking%20intracellular%20forces%20and%20changes%20to%20mechanical%20properties%20in%20mouse%20once_cell%20embryo%20development.pdf https://pubmed.ncbi.nlm.nih.gov/32451513 http://hdl.handle.net/2117/330111 https://pubmed.ncbi.nlm.nih.gov/32451513/ https://researchportal.bath.ac.uk/en/publications/tracking-intracellular-forces-and-changes-to-mechanical-propertie https://ui.adsabs.harvard.edu/abs/2020NatMa..19.1114D/abstract https://researchportal.bath.ac.uk/en/publications/tracking-intracellular-forces-and-mechanical-property-changes-in- https://upcommons.upc.edu/bitstream/2117/330111/4/Duch%2c%20M.%20Tracking%20intracellular%20forces%20and%20changes%20to%20mechanical%20properties%20in%20mouse%20once_cell%20embryo%20development.pdf https://www.nature.com/articles/s41563-020-0685-9 https://purehost.bath.ac.uk/ws/files/205660818/Duch_et_al_Nat_Mater_2020_main.pdf |
| Rights: | Springer TDM |
| Dokumentencode: | edsair.doi.dedup.....b3055e05cea4fdea5aacf97f0e3b3efd |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Cells comprise mechanically active matter that governs their functionality, but intracellular mechanics are difficult to study directly and are poorly understood. However, injected nanodevices open up opportunities to analyse intracellular mechanobiology. Here, we identify a programme of forces and changes to the cytoplasmic mechanical properties required for mouse embryo development from fertilization to the first cell division. Injected, fully internalized nanodevices responded to sperm decondensation and recondensation, and subsequent device behaviour suggested a model for pronuclear convergence based on a gradient of effective cytoplasmic stiffness. The nanodevices reported reduced cytoplasmic mechanical activity during chromosome alignment and indicated that cytoplasmic stiffening occurred during embryo elongation, followed by rapid cytoplasmic softening during cytokinesis (cell division). Forces greater than those inside muscle cells were detected within embryos. These results suggest that intracellular forces are part of a concerted programme that is necessary for development at the origin of a new embryonic life. |
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| ISSN: | 14764660 14761122 |
| DOI: | 10.1038/s41563-020-0685-9 |