Regulation of primitive hematopoiesis in zebrafish embryos by the death receptor gene
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| Název: | Regulation of primitive hematopoiesis in zebrafish embryos by the death receptor gene |
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| Autoři: | Kwan, Tommy T., Liang, Raymond, Verfaillie, Catherine, Ekker, Stephen C., Chan, Li C., Lin, Shuo, Leung, Anskar Y.H. |
| Zdroj: | Experimental Hematology. 34:27-34 |
| Informace o vydavateli: | Elsevier BV, 2006. |
| Rok vydání: | 2006 |
| Témata: | 0301 basic medicine, Caspases - drug effects - metabolism, Embryo, Nonmammalian, Receptors, Tumor Necrosis Factor - administration & dosage - genetics - metabolism, Apoptosis, Research & Experimental Medicine, Receptors, Tumor Necrosis Factor, Hemoglobins, Receptors, Zebrafish - embryology - genetics - metabolism, BMP-4, Tumor Necrosis Factor - administration & dosage - genetics - metabolism, Morpholines - administration & dosage, 1102 Cardiorespiratory Medicine and Haematology, Zebrafish, 0303 health sciences, Nonmammalian, Caspase 3, INDUCTION, Gene Expression Regulation, Developmental, Hematology, APOPTOSIS, Medicine, Research & Experimental, Embryo, Glycoproteins - administration & dosage, Caspases, Intercellular Signaling Peptides and Proteins, Intercellular Signaling Peptides and Proteins - administration & dosage, 3201 Cardiovascular medicine and haematology, Life Sciences & Biomedicine, Developmental - drug effects, Morpholines, Apoptosis - drug effects, Immunology, Hemoglobins - biosynthesis - drug effects, 03 medical and health sciences, Gene Expression Regulation, Developmental - drug effects, Animals, Glycoproteins, Science & Technology, Animal, MESODERM, Hematopoiesis, Disease Models, Animal, Gene Expression Regulation, TRANSGENIC ZEBRAFISH, Disease Models, CELLS, ERYTHROPOIESIS, Hematopoiesis - drug effects - genetics - physiology |
| Popis: | We investigated the regulatory mechanism of primitive hematopoiesis in zebrafish (Danio rerio) embryos with particular reference to the role of a death receptor (zDR) gene, based on a morpholino (MO) knockdown approach.MOs targeting the zDR and chordin (Chd) were injected into naturally spawned embryos at one- to four-cell stage. A random sequence (RS) MO was used as a control. Effects on hemoglobin formation (Hb), apoptosis, and lineage-specific gene expression were examined. Embryos injected with zDR, Chd, and RS-MOs were denoted zDR(mo), zChd(mo), and zRS(mo), respectively. Those co-injected with Chd+zDR-MOs and Chd+RS-MOs were abbreviated zChd+DR(mo) and zChd+RS(mo).zDR mRNA expression was restricted to the intermediate cell mass of wild-type (WT) and zChd(mo) embryos. At 48 hours postfertilization, zDR(mo) embryos showed increased Hb compared with WT or zRS(mo) embryos (2.36 x 10(-2) +/- 1.13 x 10(-3) vs 1.85 x 10(-2) +/- 5.60 x 10(-4) vs 1.79 x 10(-2) +/- 1.31 x 10(-3) U, p < 0.05). zChd+DR(mo) embryos also showed increased Hb compared with zChd(mo) or zChd+RS(mo) embryos (4.60 x 10(-2) +/- 2.79 x 10(-3) vs 3.17 x 10(-2) +/- 1.07 x 10(-3) vs 3.05 x 10(-2) +/- 1.25 x 10(-3) U, p < 0.05). zDR-MO reduced apoptosis, as shown by reduced terminal transferase-mediated dUTP nick end-labeling staining in zChd+DR(mo) compared with zChd+RS(mo) embryos and caspase-3 activity in zDR(mo) vs zRS(mo) (0.525 +/- 0.094 vs 0.953 +/- 0.113 U, p < 0.05), and zChd+DR(mo) vs zChd+RS(mo) embryos (0.247 +/- 0.121 vs 1.180 +/- 0.082, p < 0.05). zChd+DR(mo) embryos showed upregulation of erythroid-specific embryonic hemoglobin gene expression but not that of a myeloid-specific myeloperoxidase gene.Knockdown of zDR in zebrafish embryos decreased apoptosis and increased Hb, suggesting that zDR may regulate primitive hematopoiesis during development. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0301-472X |
| DOI: | 10.1016/j.exphem.2005.09.017 |
| Přístupová URL adresa: | https://lirias.kuleuven.be/bitstream/123456789/34751/1/Kwan%20-%20Experimental%20Hematology%20-%202006.pdf https://pubmed.ncbi.nlm.nih.gov/16413388 https://pubmed.ncbi.nlm.nih.gov/16413388/ https://europepmc.org/abstract/MED/16413388 https://www.sciencedirect.com/science/article/pii/S0301472X05004662 https://mayoclinic.pure.elsevier.com/en/publications/regulation-of-primitive-hematopoiesis-in-zebrafish-embryos-by-the https://www.ncbi.nlm.nih.gov/pubmed/16413388 https://www.exphem.org/article/S0301-472X(05)00466-2/fulltext https://lirias.kuleuven.be/handle/123456789/34751 https://doi.org/10.1016/j.exphem.2005.09.017 http://hdl.handle.net/10722/78487 |
| Rights: | Elsevier Non-Commercial |
| Přístupové číslo: | edsair.doi.dedup.....b2527fd0602e43a6aaf8fbb28331d807 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | We investigated the regulatory mechanism of primitive hematopoiesis in zebrafish (Danio rerio) embryos with particular reference to the role of a death receptor (zDR) gene, based on a morpholino (MO) knockdown approach.MOs targeting the zDR and chordin (Chd) were injected into naturally spawned embryos at one- to four-cell stage. A random sequence (RS) MO was used as a control. Effects on hemoglobin formation (Hb), apoptosis, and lineage-specific gene expression were examined. Embryos injected with zDR, Chd, and RS-MOs were denoted zDR(mo), zChd(mo), and zRS(mo), respectively. Those co-injected with Chd+zDR-MOs and Chd+RS-MOs were abbreviated zChd+DR(mo) and zChd+RS(mo).zDR mRNA expression was restricted to the intermediate cell mass of wild-type (WT) and zChd(mo) embryos. At 48 hours postfertilization, zDR(mo) embryos showed increased Hb compared with WT or zRS(mo) embryos (2.36 x 10(-2) +/- 1.13 x 10(-3) vs 1.85 x 10(-2) +/- 5.60 x 10(-4) vs 1.79 x 10(-2) +/- 1.31 x 10(-3) U, p < 0.05). zChd+DR(mo) embryos also showed increased Hb compared with zChd(mo) or zChd+RS(mo) embryos (4.60 x 10(-2) +/- 2.79 x 10(-3) vs 3.17 x 10(-2) +/- 1.07 x 10(-3) vs 3.05 x 10(-2) +/- 1.25 x 10(-3) U, p < 0.05). zDR-MO reduced apoptosis, as shown by reduced terminal transferase-mediated dUTP nick end-labeling staining in zChd+DR(mo) compared with zChd+RS(mo) embryos and caspase-3 activity in zDR(mo) vs zRS(mo) (0.525 +/- 0.094 vs 0.953 +/- 0.113 U, p < 0.05), and zChd+DR(mo) vs zChd+RS(mo) embryos (0.247 +/- 0.121 vs 1.180 +/- 0.082, p < 0.05). zChd+DR(mo) embryos showed upregulation of erythroid-specific embryonic hemoglobin gene expression but not that of a myeloid-specific myeloperoxidase gene.Knockdown of zDR in zebrafish embryos decreased apoptosis and increased Hb, suggesting that zDR may regulate primitive hematopoiesis during development. |
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| ISSN: | 0301472X |
| DOI: | 10.1016/j.exphem.2005.09.017 |