Decreased Stimulation of Adenylate Cyclase by Growth Hormone-Releasing Factor in the Anterior Pituitary of Old Rats
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| Titel: | Decreased Stimulation of Adenylate Cyclase by Growth Hormone-Releasing Factor in the Anterior Pituitary of Old Rats |
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| Autoren: | Robberecht, Patrick, Gillard, Michel, Waelbroeck, Magali, Camus, Jean Claude, De Neef, Philippe, Christophe, Jean |
| Quelle: | Neuroendocrinology. 44:429-432 |
| Verlagsinformationen: | S. Karger AG, 1986. |
| Publikationsjahr: | 1986 |
| Schlagwörter: | Male, 0301 basic medicine, Aging, Inbred Strains, Forskolin -- pharmacology, Anterior -- enzymology, Growth Hormone-Releasing Hormone, 03 medical and health sciences, 0302 clinical medicine, Pituitary Gland, Anterior, Growth Hormone-Releasing Hormone -- pharmacology, Animals, Guanylyl Imidodiphosphate, Colforsin, Aging -- metabolism, Rats, Inbred Strains, Sciences bio-médicales et agricoles, Guanylyl Imidodiphosphate -- pharmacology, Vasoactive Intestinal Peptide -- pharmacology, Rats, Pituitary Gland, Adenylate Cyclase -- metabolism, Sodium Fluoride, Guanosine Triphosphate -- pharmacology, Guanosine Triphosphate, Sodium Fluoride -- pharmacology, Adenylyl Cyclases, Vasoactive Intestinal Peptide |
| Beschreibung: | Adenylate cyclase activity was studied in anterior pituitary homogenates from young adult (6 months) and old (20 and 24 months) male rats. Basal, NaF-, GTP-, 5'-guanylimidodiphosphate (Gpp(NH)p)- and forskolin-stimulated activities were similar in the three groups, implying that the stimulatory guanyl nucleotide regulatory binding site (NS) and the catalytical unit were unaffected by aging. Vasoactive intestinal peptide (VIP)-stimulated adenylate cyclase activity was also identical in the three groups. The efficacy (i.e., the maximum effect) of growth hormone-releasing factor [GRF(1-29)-NH2] on adenylate activity was reduced by 45-49% in old and senescent rats with no change in peptide potency (i.e., the concentration required for half-maximal enzyme stimulation). These results suggest that aging induced a selective loss of functional GRF receptors but influenced neither the coupling between receptors, NS and the catalytic unit nor the efficacy of the catalytic unit per se. |
| Publikationsart: | Article |
| Dateibeschreibung: | No full-text files |
| Sprache: | English |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000124682 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/3102990 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/148234/Details http://www.ncbi.nlm.nih.gov/pubmed/3102990 |
| Rights: | URL: https://www.karger.com/Services/SiteLicenses |
| Dokumentencode: | edsair.doi.dedup.....aa6f2bf53a393c80ac5c5d0cdc507979 |
| Datenbank: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | Adenylate cyclase activity was studied in anterior pituitary homogenates from young adult (6 months) and old (20 and 24 months) male rats. Basal, NaF-, GTP-, 5'-guanylimidodiphosphate (Gpp(NH)p)- and forskolin-stimulated activities were similar in the three groups, implying that the stimulatory guanyl nucleotide regulatory binding site (NS) and the catalytical unit were unaffected by aging. Vasoactive intestinal peptide (VIP)-stimulated adenylate cyclase activity was also identical in the three groups. The efficacy (i.e., the maximum effect) of growth hormone-releasing factor [GRF(1-29)-NH2] on adenylate activity was reduced by 45-49% in old and senescent rats with no change in peptide potency (i.e., the concentration required for half-maximal enzyme stimulation). These results suggest that aging induced a selective loss of functional GRF receptors but influenced neither the coupling between receptors, NS and the catalytic unit nor the efficacy of the catalytic unit per se. |
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| ISSN: | 14230194 00283835 |
| DOI: | 10.1159/000124682 |