Zobrazit v EDS

Effects of Y-27632, a Rho-associated Kinase Inhibitor, on Human Corneal Endothelial Cells Cultured by Isolating Human Corneal Endothelial Progenitor Cells

Uloženo v:
Podrobná bibliografie
Název: Effects of Y-27632, a Rho-associated Kinase Inhibitor, on Human Corneal Endothelial Cells Cultured by Isolating Human Corneal Endothelial Progenitor Cells
Autoři: Shin, Haeeun, Min, Joon Ki, Kim, Na Rae, Seo, Kyoung Yul, Chin, Hee Seung, Lee, Soyoung, Jung, Ji Won
Přispěvatelé: Haeeun Shin, Joon Ki Min, Na Rae Kim, Kyoung Yul Seo, Hee Seung Chin, Soyoung Lee, Ji Won Jung, Seo, Kyoung Yul
Zdroj: Korean J Ophthalmol
Informace o vydavateli: Korean Ophthalmological Society, 2023.
Rok vydání: 2023
Témata: 0301 basic medicine, Human corneal endothelial progenitor cells, Corneal endothelial dysfunction, Cells, Rho-associated kinase inhibitor, 03 medical and health sciences, 0302 clinical medicine, Humans, Endothelium, Endothelial Progenitor Cells* / drug effects, Cells, Cultured, Cell Proliferation, Endothelial Progenitor Cells, rho-Associated Kinases, Cultured, Endothelium, Corneal, Corneal, Amides / pharmacology, Amides, 3. Good health, Human corneal endothelial cells, rho-Associated Kinases* / antagonists & inhibitors, Differentiated human corneal endothelial progenitor cells, Original Article
Popis: Purpose: Human corneal endothelial progenitor cells (HCEPs), which has been selectively isolated and differentiated into human corneal endothelial cells (HCECs), are crucial for repairing corneal endothelial damage. In this study, we evaluated the roles of a Rho-assisted kinase (ROCK) inhibitor, Y-27632, on the isolation and expansion of HCEPs, and assessed the in vitro effects of different concentrations of Y-27632 on the differentiated HCEPs.Methods: HCEPs were isolated and expanded in a medium with and without 10μM Y-27632, and then differentiated into HCECs in a medium with fetal bovine serum. The characteristics of HCEPs and differentiated HCEPs were confirmed by immunofluorescence staining. The proliferation, viability, morphology, and wound-healing ability of differentiated HCEPs were assessed in the presence of different concentrations of Y-27632.Results: Y-27632 enabled the isolation and expansion of HCEPs from the corneal endothelium. The differentiated HCEPs showed an optimal increase in proliferation and survival in the presence of 10μM Y-27632. As the concentration of Y-27632 increased, differentiated HCEPs became elongated, and actin filaments were redistributed to the periphery of cells. Y-27632 also caused a concentration-dependent enhancement in the wound-healing ability of differentiated HCEPs.Conclusions: Y-27632 enabled the isolation and expansion of HCEPs. It also enhanced the proliferation, viability, and migration of differentiated HCEPs.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 2092-9382
1011-8942
DOI: 10.3341/kjo.2022.0133
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/36549303
Rights: CC BY NC
CC BY NC ND
URL: http://creativecommons.org/licenses/by-nc/4.0/This is an Open Access journal distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (http://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Přístupové číslo: edsair.doi.dedup.....aa6229394fdb5f014b64a8d63df2abc6
Databáze: OpenAIRE
Popis
Abstrakt:Purpose: Human corneal endothelial progenitor cells (HCEPs), which has been selectively isolated and differentiated into human corneal endothelial cells (HCECs), are crucial for repairing corneal endothelial damage. In this study, we evaluated the roles of a Rho-assisted kinase (ROCK) inhibitor, Y-27632, on the isolation and expansion of HCEPs, and assessed the in vitro effects of different concentrations of Y-27632 on the differentiated HCEPs.Methods: HCEPs were isolated and expanded in a medium with and without 10μM Y-27632, and then differentiated into HCECs in a medium with fetal bovine serum. The characteristics of HCEPs and differentiated HCEPs were confirmed by immunofluorescence staining. The proliferation, viability, morphology, and wound-healing ability of differentiated HCEPs were assessed in the presence of different concentrations of Y-27632.Results: Y-27632 enabled the isolation and expansion of HCEPs from the corneal endothelium. The differentiated HCEPs showed an optimal increase in proliferation and survival in the presence of 10μM Y-27632. As the concentration of Y-27632 increased, differentiated HCEPs became elongated, and actin filaments were redistributed to the periphery of cells. Y-27632 also caused a concentration-dependent enhancement in the wound-healing ability of differentiated HCEPs.Conclusions: Y-27632 enabled the isolation and expansion of HCEPs. It also enhanced the proliferation, viability, and migration of differentiated HCEPs.
ISSN:20929382
10118942
DOI:10.3341/kjo.2022.0133