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Increased expression of pentraxin 3 in placental tissues from patients with unexplained recurrent pregnancy loss

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Titel: Increased expression of pentraxin 3 in placental tissues from patients with unexplained recurrent pregnancy loss
Autoren: Zeybek, S., Tepeli, E., Çetin, Gökhan Ozan, Caner, Vildan, Şenol, Hande, Yildirim, B., Bağcı, Gülseren
Quelle: Balkan J Med Genet
Balkan Journal of Medical Genetics, Vol 22, Iss 1, Pp 21-28 (2019)
Verlagsinformationen: Walter de Gruyter GmbH, 2019.
Publikationsjahr: 2019
Schlagwörter: placenta tissue, placentai expression, unexplained recurrent pregnancy loss (urpl), QH426-470, Article, live birth, pentraxin 3 (ptx3), 03 medical and health sciences, 0302 clinical medicine, recurrent abortion, Genetics, controlled study, human, pentraxin 3, gestational age, protein expression, Inflammation, clinical article, Placentai expression, messenger RNA, Unexplained recurrent pregnancy loss (URPL), medically unexplained symptom, human tissue, 3. Good health, fetus, female, protein expression level, maternal age, real time polymerase chain reaction, inflammation, immunohistochemistry, Pentraxin 3 (PTX3), Original Article, Real-time polymerase chain reaction (RT-PCR), real-time polymerase chain reaction (rt-pcr)
Beschreibung: Pentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a evolutionarily conserved multimeric pattern recognition receptor involved in the humoral component of the innate immune system. Pentraxin 3 is released when tissue is stressed or damaged, and interacts with many different ligands. Pentraxin 3 exerts a pivotal role both as a regulator and as an indicator of inflammatory response in the pathogenesis of many diseases such as sepsis, vasculitis and preeclampsia. Uncontrolled inflammatory response is considered a major cause of unexplained recurrent pregnancy loss (URPL). We determined the PTX3 messenger ribonucleic acid (mRNA) and protein expression levels in placentai tissues from 50 women with URPL, and made comparison with those in 50 age-matched control subjects. In quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry analyses, PTX3 mRNA and protein levels, respectively, were significantly increased in URPL patients compared with their respective controls (p = 0.0001). Although no significant correlations were identified between PTX3 expression levels and clinical parameters such as maternal age, numbers of previous pregnancy losses, and gestational age at miscarriage, PTX3 mRNA expression was significantly higher in patients with no live births than in women with previous live births (p = 0.0001). Our study suggests that tissue-specific expression of PTX3 is associated with URPL. Further larger studies are required to determine whether PTX3 expression can be used as a biomarker to manage URPL in routine clinical practice.
Publikationsart: Article
Other literature type
Dateibeschreibung: application/pdf
Sprache: English
ISSN: 1311-0160
DOI: 10.2478/bjmg-2019-0002
Zugangs-URL: https://content.sciendo.com/downloadpdf/journals/bjmg/22/1/article-p21.pdf
https://pubmed.ncbi.nlm.nih.gov/31523616
https://doaj.org/article/92d4075ddcde41cdae7e3aad94763f2d
https://pubmed.ncbi.nlm.nih.gov/31523616/
https://content.sciendo.com/view/journals/bjmg/22/1/article-p21.xml
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714334
https://europepmc.org/article/MED/31523616
http://sciendo.com/article/10.2478/bjmg-2019-0002
https://hdl.handle.net/11499/30083
https://doi.org/10.2478/bjmg-2019-0002
Rights: CC BY NC ND
Dokumentencode: edsair.doi.dedup.....a93b9f0590008bf1c0a0ca8ac286d6b3
Datenbank: OpenAIRE
Beschreibung
Abstract:Pentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a evolutionarily conserved multimeric pattern recognition receptor involved in the humoral component of the innate immune system. Pentraxin 3 is released when tissue is stressed or damaged, and interacts with many different ligands. Pentraxin 3 exerts a pivotal role both as a regulator and as an indicator of inflammatory response in the pathogenesis of many diseases such as sepsis, vasculitis and preeclampsia. Uncontrolled inflammatory response is considered a major cause of unexplained recurrent pregnancy loss (URPL). We determined the PTX3 messenger ribonucleic acid (mRNA) and protein expression levels in placentai tissues from 50 women with URPL, and made comparison with those in 50 age-matched control subjects. In quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry analyses, PTX3 mRNA and protein levels, respectively, were significantly increased in URPL patients compared with their respective controls (p = 0.0001). Although no significant correlations were identified between PTX3 expression levels and clinical parameters such as maternal age, numbers of previous pregnancy losses, and gestational age at miscarriage, PTX3 mRNA expression was significantly higher in patients with no live births than in women with previous live births (p = 0.0001). Our study suggests that tissue-specific expression of PTX3 is associated with URPL. Further larger studies are required to determine whether PTX3 expression can be used as a biomarker to manage URPL in routine clinical practice.
ISSN:13110160
DOI:10.2478/bjmg-2019-0002