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Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

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Názov: Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells
Autori: Skuland, Tonje, Grytting, Vegard S., Låg, Marit, Jørgensen, Rikke B., Snilsberg, Brynhild, Leseman, Daan L. A. C., Kubátová, Alena, Emond, Jessica, Cassee, Flemming R., Holme, Jørn A., Øvrevik, Johan, Refsnes, Magne
Prispievatelia: Open Repository DS7.6 Demo
Zdroj: Part Fibre Toxicol
Particle and Fibre Toxicology, Vol 19, Iss 1, Pp 1-20 (2022)
Particle and Fibre Toxicology
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2022.
Rok vydania: 2022
Predmety: Particulate Matter/toxicity, 0301 basic medicine, 0303 health sciences, Research, Mineral particles, Epithelial Cells, HD7260-7780.8, Carbon, 6. Clean water, 03 medical and health sciences, 13. Climate action, Urban air particulate matter, RA1190-1270, Toxicology. Poisons, 11. Sustainability, Stone particles, Cytokines, Humans, Industrial hygiene. Industrial welfare, Particulate Matter, Seasons, Oxidative potential, Epithelial lung cells, Organic carbon
Popis: Background Traffic particulate matter (PM) comprises a mixture of particles from fuel combustion and wear of road pavement, tires and brakes. In countries with low winter temperatures the relative contribution of mineral-rich PM from road abrasion may be especially high due to use of studded tires during winter season. The aim of the present study was to sample and characterize size-fractioned PM from two road tunnels paved with different stone materials in the asphalt, and to compare the pro-inflammatory potential of these fractions in human bronchial epithelial cells (HBEC3-KT) in relation to physicochemical characteristics. Methods The road tunnel PM was collected with a vacuum pump and a high-volume cascade impactor sampler. PM was sampled during winter, both during humid and dry road surface conditions, and before and after cleaning the tunnels. Samples were analysed for hydrodynamic size distribution, content of elemental carbon (EC), organic carbon (OC) and endotoxin, and the capacity for acellular generation of reactive oxygen species. Cytotoxicity and pro-inflammatory responses were assessed in HBEC3-KT cells after exposure to coarse (2.5–10 μm), fine (0.18–2.5 μm) and ultrafine PM (≤ 0.18 μm), as well as particles from the respective stone materials used in the pavement. Results The pro-inflammatory potency of the PM samples varied between road tunnels and size fractions, but showed more marked responses than for the stone materials used in asphalt of the respective tunnels. In particular, fine samples showed significant increases as low as 25 µg/mL (2.6 µg/cm2) and were more potent than coarse samples, while ultrafine samples showed more variable responses between tunnels, sampling conditions and endpoints. The most marked responses were observed for fine PM sampled during humid road surface conditions. Linear correlation analysis showed that particle-induced cytokine responses were correlated to OC levels, while no correlations were observed for other PM characteristics. Conclusions The pro-inflammatory potential of fine road tunnel PM sampled during winter season was high compared to coarse PM. The differences between the PM-induced cytokine responses were not related to stone materials in the asphalt. However, the ratio of OC to total PM mass was associated with the pro-inflammatory potential.
Druh dokumentu: Article
Other literature type
Popis súboru: application/pdf
Jazyk: English
ISSN: 1743-8977
DOI: 10.1186/s12989-022-00488-5
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/35787286
https://doaj.org/article/87f98df4256845e190bb6dc9a0ca864e
https://hdl.handle.net/11250/3046203
https://hdl.handle.net/10029/625928
Rights: CC BY
Prístupové číslo: edsair.doi.dedup.....a8688c23ee5c9d0c7d057f0214c645ee
Databáza: OpenAIRE
Popis
Abstrakt:Background Traffic particulate matter (PM) comprises a mixture of particles from fuel combustion and wear of road pavement, tires and brakes. In countries with low winter temperatures the relative contribution of mineral-rich PM from road abrasion may be especially high due to use of studded tires during winter season. The aim of the present study was to sample and characterize size-fractioned PM from two road tunnels paved with different stone materials in the asphalt, and to compare the pro-inflammatory potential of these fractions in human bronchial epithelial cells (HBEC3-KT) in relation to physicochemical characteristics. Methods The road tunnel PM was collected with a vacuum pump and a high-volume cascade impactor sampler. PM was sampled during winter, both during humid and dry road surface conditions, and before and after cleaning the tunnels. Samples were analysed for hydrodynamic size distribution, content of elemental carbon (EC), organic carbon (OC) and endotoxin, and the capacity for acellular generation of reactive oxygen species. Cytotoxicity and pro-inflammatory responses were assessed in HBEC3-KT cells after exposure to coarse (2.5–10 μm), fine (0.18–2.5 μm) and ultrafine PM (≤ 0.18 μm), as well as particles from the respective stone materials used in the pavement. Results The pro-inflammatory potency of the PM samples varied between road tunnels and size fractions, but showed more marked responses than for the stone materials used in asphalt of the respective tunnels. In particular, fine samples showed significant increases as low as 25 µg/mL (2.6 µg/cm2) and were more potent than coarse samples, while ultrafine samples showed more variable responses between tunnels, sampling conditions and endpoints. The most marked responses were observed for fine PM sampled during humid road surface conditions. Linear correlation analysis showed that particle-induced cytokine responses were correlated to OC levels, while no correlations were observed for other PM characteristics. Conclusions The pro-inflammatory potential of fine road tunnel PM sampled during winter season was high compared to coarse PM. The differences between the PM-induced cytokine responses were not related to stone materials in the asphalt. However, the ratio of OC to total PM mass was associated with the pro-inflammatory potential.
ISSN:17438977
DOI:10.1186/s12989-022-00488-5