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Cytotoxic impact of nicotine products on periodontal ligament cells

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Název: Cytotoxic impact of nicotine products on periodontal ligament cells
Autoři: Wiesmann-Imilowski, Nadine, Becker, Philipp, Gielisch, Matthias W., Ziebolz, Dirk, Vermehren, Franziska, Bitschnau, Marian, Langguth, Nils, Brieger, Jürgen, Deschner, James, Kämmerer, Peer W.
Přispěvatelé: Johannes Gutenberg-Universität Mainz
Zdroj: Clin Oral Investig
Informace o vydavateli: Springer Science and Business Media LLC, 2024.
Rok vydání: 2024
Témata: Aerosols, Nicotine, Smoke/adverse effects [MeSH], Periodontal Ligament/cytology [MeSH], Nicotine/pharmacology [MeSH], Aerosols [MeSH], E-Cigarette Vapor/toxicity [MeSH], Flow Cytometry [MeSH], Humans [MeSH], Periodontal ligament cells, Apoptosis/drug effects [MeSH], Electronic Nicotine Delivery Systems [MeSH], In Vitro Techniques [MeSH], Nicotine/toxicity [MeSH], Periodontal Ligament/drug effects [MeSH], Periodontal health, Smoking, Electronic cigarette, Research, Tobacco Products/toxicity [MeSH], Heated tobacco product, Cells, Cultured [MeSH], Cell Proliferation/drug effects [MeSH], Periodontal Ligament, 610 Medizin, Apoptosis, Tobacco Products, Electronic Nicotine Delivery Systems, In Vitro Techniques, Flow Cytometry, 01 natural sciences, 03 medical and health sciences, 0302 clinical medicine, E-Cigarette Vapor, 610 Medical sciences, Smoke, Humans, Cells, Cultured, Cell Proliferation, 0105 earth and related environmental sciences
Popis: Objectives The primary objective of this in vitro experiment was an assessment of proliferative capacity, metabolic activity, and potential cellular detriment of human periodontal ligament cells (hPDL) exposed to cigarette smoke (CS), electronic cigarette vapor (eCV), and heated tobacco product aerosol (HTP), or air (control). Materials and methods Using a CAD/CAM-designed exposition chamber, hPDL were exposed to CS, eCV, HTP, or air (control) based on the Health Canada Intense Smoking Regime. Cell proliferation, metabolic activity, and cellular detriment were assessed at various time points. Results Compared to the control, hPDL exposed to CS exhibited significantly decreased cell numbers at all time points. HTP exposure led to reduced cell numbers 48 h and 72 h post-exposure, while eCV-exposed cells showed no significant decrease. The metabolic activity of eCV-treated hPDL was slightly reduced at 7 h but recovered at 24 h and 48 h. In contrast, CS-treated cells exhibited significantly decreased metabolic activity at 24 h and 48 h, and HTP-exposed cells showed a significant decrease after 48 h. Flow cytometry indicated both apoptotic and necrotic cell death following CS exposure, with necrotic cell death being more pronounced. Conclusions eCV and HTP demonstrated comparatively reduced detrimental effects on hPDL compared to CS. Clinical relevance : The findings suggest that conventional cigarette smoke poses a substantial risk to periodontal health by significantly impairing cell proliferation and metabolic activity. However, alternatives such as eCV and HTP may offer a comparatively reduced risk.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 1436-3771
DOI: 10.1007/s00784-024-05797-x
DOI: 10.25358/openscience-12314
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/38922383
https://openscience.ub.uni-mainz.de/handle/20.500.12030/12335
https://doi.org/10.25358/openscience-12314
https://repository.publisso.de/resource/frl:6522613
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....a80ab33217f381f0e61e8022a233f044
Databáze: OpenAIRE
Popis
Abstrakt:Objectives The primary objective of this in vitro experiment was an assessment of proliferative capacity, metabolic activity, and potential cellular detriment of human periodontal ligament cells (hPDL) exposed to cigarette smoke (CS), electronic cigarette vapor (eCV), and heated tobacco product aerosol (HTP), or air (control). Materials and methods Using a CAD/CAM-designed exposition chamber, hPDL were exposed to CS, eCV, HTP, or air (control) based on the Health Canada Intense Smoking Regime. Cell proliferation, metabolic activity, and cellular detriment were assessed at various time points. Results Compared to the control, hPDL exposed to CS exhibited significantly decreased cell numbers at all time points. HTP exposure led to reduced cell numbers 48 h and 72 h post-exposure, while eCV-exposed cells showed no significant decrease. The metabolic activity of eCV-treated hPDL was slightly reduced at 7 h but recovered at 24 h and 48 h. In contrast, CS-treated cells exhibited significantly decreased metabolic activity at 24 h and 48 h, and HTP-exposed cells showed a significant decrease after 48 h. Flow cytometry indicated both apoptotic and necrotic cell death following CS exposure, with necrotic cell death being more pronounced. Conclusions eCV and HTP demonstrated comparatively reduced detrimental effects on hPDL compared to CS. Clinical relevance : The findings suggest that conventional cigarette smoke poses a substantial risk to periodontal health by significantly impairing cell proliferation and metabolic activity. However, alternatives such as eCV and HTP may offer a comparatively reduced risk.
ISSN:14363771
DOI:10.1007/s00784-024-05797-x