Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants
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| Název: | Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants |
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| Autoři: | Benjamin Ingham, Rehana Sung, Phil Kay, Katherine Hollywood, Phavit Wongsirichot, Alistair Veitch, James Winterburn |
| Zdroj: | J Ind Microbiol Biotechnol Ingham, B, Sung, R, Kaye, P, Hollywood, K, Wongsirichot, P, Veitch, A & Winterburn, J 2024, 'Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants.', Journal of Industrial Microbiology & Biotechnology. https://doi.org/10.1093/jimb/kuae021 |
| Informace o vydavateli: | Oxford University Press (OUP), 2024. |
| Rok vydání: | 2024 |
| Témata: | Surface-Active Agents, Solvent extraction, Quantification, Gravimetric, Sophorolipid, Biosurfactants, Fermentation, Oleic Acids, Biotechnology Methods, Chromatography, High Pressure Liquid, High performance liquid chromatography, Culture Media |
| Popis: | To determine the performance of a sophorolipid biosurfactant production process, it is important to have accurate and specific analytical techniques in place. Among the most popular are the anthrone assay, gravimetric quantification (hexane:ethyl acetate extraction), and high-performance liquid chromatography (HPLC). The choice of analytical tool varies depending on cost, availability, and ease of use; however, these techniques have never been compared directly against one another. In this work, 75 fermentation broths with varying product/substrate concentrations were comprehensively tested with the 3 techniques and compared. HPLC–ultraviolet detection (198 nm) was capable of quantifying C18:1 subterminal hydroxyl diacetylated lactonic sophorolipid down to a lower limit of 0.3 g/L with low variability ( |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1093/jimb/kuae021 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/38906848 https://research.manchester.ac.uk/en/publications/5721b658-f00c-4c9d-b54a-5ff8f26047ac https://doi.org/10.1093/jimb/kuae021 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Přístupové číslo: | edsair.doi.dedup.....a5e02a7d9112f182e1a0dbbf6e1c747c |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | To determine the performance of a sophorolipid biosurfactant production process, it is important to have accurate and specific analytical techniques in place. Among the most popular are the anthrone assay, gravimetric quantification (hexane:ethyl acetate extraction), and high-performance liquid chromatography (HPLC). The choice of analytical tool varies depending on cost, availability, and ease of use; however, these techniques have never been compared directly against one another. In this work, 75 fermentation broths with varying product/substrate concentrations were comprehensively tested with the 3 techniques and compared. HPLC–ultraviolet detection (198 nm) was capable of quantifying C18:1 subterminal hydroxyl diacetylated lactonic sophorolipid down to a lower limit of 0.3 g/L with low variability ( |
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| ISSN: | 14765535 13675435 |
| DOI: | 10.1093/jimb/kuae021 |