Zobrazit v EDS

The genetic relationship between human and pet isolates: a core genome multilocus sequence analysis of multidrug-resistant bacteria

Uloženo v:
Podrobná bibliografie
Název: The genetic relationship between human and pet isolates: a core genome multilocus sequence analysis of multidrug-resistant bacteria
Autoři: Genath, Antonia, Hackmann, Carolin, Denkel, Luisa, Weber, Anna, Maechler, Friederike, Kola, Axel, Schwarz, Stefan, Gastmeier, Petra, Leistner, Rasmus
Zdroj: Antimicrob Resist Infect Control
Antimicrobial Resistance and Infection Control, Vol 13, Iss 1, Pp 1-13 (2024)
Informace o vydavateli: Springer Science and Business Media LLC, 2024.
Rok vydání: 2024
Témata: Methicillin-Resistant Staphylococcus aureus, Landwirtschaft und verwandte Bereiche, Research, Genetic Variation, Multidrug-resistance, Infectious and parasitic diseases, RC109-216, Pets, Microbial Sensitivity Tests, Molecular typing, Anti-Bacterial Agents, Vancomycin-Resistant Enterococci, Dogs, Pets/microbiology [MeSH], Cats [MeSH], Humans [MeSH], One Health [MeSH], Genome, Bacterial [MeSH], Vancomycin-Resistant Enterococci/genetics [MeSH], Animals [MeSH], Methicillin-Resistant Staphylococcus aureus/classification [MeSH], Dogs [MeSH], Methicillin-Resistant Staphylococcus aureus/genetics [MeSH], Anti-Bacterial Agents/pharmacology [MeSH], Germany [MeSH], Methicillin-Resistant Staphylococcus aureus/drug effects [MeSH], Multilocus Sequence Typing [MeSH], One Health, Genetic Variation [MeSH], Microbial Sensitivity Tests [MeSH], cgMLST, Drug Resistance, Multiple, Bacterial/genetics [MeSH], Drug Resistance, Multiple, Bacterial, Germany, Cats, Humans, Animals, Genome, Bacterial, Multilocus Sequence Typing
Popis: Introduction The global increase of multidrug-resistant organisms (MDROs) is one of the most urgent public health threats affecting both humans and animals. The One Health concept emphasizes the interconnectedness of human, animal and environmental health and highlights the need for integrated approaches to combat antimicrobial resistance (AMR). Although the sharing of environments and antimicrobial agents between companion animals and humans poses a risk for MDRO transmission, companion animals have been studied to a lesser extent than livestock animals. This study therefore used core genome multilocus sequence typing (cgMLST) to investigate the genetic relationships and putative transmission of MDROs between humans and pets. Methods This descriptive integrated typing study included 252 human isolates, 53 dog isolates and 10 cat isolates collected from 2019 to 2022 at the Charité University Hospital in Berlin, Germany. CgMLST was performed to characterize methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci and multidrug-resistant gram-negative bacteria. The genetic diversity of the MDROs of the different host populations was determined and compared based on sequence type and core genome complex type. Results Within this study the majority of samples from pets and humans was genetically distinct. However, for some isolates, the number of allelic differences identified by cgMLST was low. Two cases of putative household transmission or shared source of VR E. faecium and MDR E. coli between humans and pets were documented. Conclusions The interaction between humans and their pets appears to play a minor role in the spread of the MDROs studied. However, further research is needed. This study emphasizes the importance of comprehensive molecular surveillance and a multidisciplinary One Health approach to understand and contain the spread of MDROs in human and animal populations. Trial Registration The study is registered with the German Clinical Trials Register (DRKS00030009).
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 2047-2994
DOI: 10.1186/s13756-024-01457-7
DOI: 10.17169/refubium-46100
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39304920
https://doaj.org/article/b836588d625c40c7a298a389f2ff25a9
https://repository.publisso.de/resource/frl:6520379
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....a348abd9f8af8aa993a183e3f608f7b0
Databáze: OpenAIRE
Popis
Abstrakt:Introduction The global increase of multidrug-resistant organisms (MDROs) is one of the most urgent public health threats affecting both humans and animals. The One Health concept emphasizes the interconnectedness of human, animal and environmental health and highlights the need for integrated approaches to combat antimicrobial resistance (AMR). Although the sharing of environments and antimicrobial agents between companion animals and humans poses a risk for MDRO transmission, companion animals have been studied to a lesser extent than livestock animals. This study therefore used core genome multilocus sequence typing (cgMLST) to investigate the genetic relationships and putative transmission of MDROs between humans and pets. Methods This descriptive integrated typing study included 252 human isolates, 53 dog isolates and 10 cat isolates collected from 2019 to 2022 at the Charité University Hospital in Berlin, Germany. CgMLST was performed to characterize methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci and multidrug-resistant gram-negative bacteria. The genetic diversity of the MDROs of the different host populations was determined and compared based on sequence type and core genome complex type. Results Within this study the majority of samples from pets and humans was genetically distinct. However, for some isolates, the number of allelic differences identified by cgMLST was low. Two cases of putative household transmission or shared source of VR E. faecium and MDR E. coli between humans and pets were documented. Conclusions The interaction between humans and their pets appears to play a minor role in the spread of the MDROs studied. However, further research is needed. This study emphasizes the importance of comprehensive molecular surveillance and a multidisciplinary One Health approach to understand and contain the spread of MDROs in human and animal populations. Trial Registration The study is registered with the German Clinical Trials Register (DRKS00030009).
ISSN:20472994
DOI:10.1186/s13756-024-01457-7