Zobrazit v EDS

Beneficial impact of zinc supplementation on the collagen in the bone tissue of cadmium‐exposed rats

Uloženo v:
Podrobná bibliografie
Název: Beneficial impact of zinc supplementation on the collagen in the bone tissue of cadmium‐exposed rats
Autoři: Edyta Andrulewicz‐Botulińska, Róża Wiśniewska, Malgorzata M. Brzóska, Joanna Rogalska, Anna Galicka
Zdroj: Journal of Applied Toxicology. 38:996-1007
Informace o vydavateli: Wiley, 2018.
Rok vydání: 2018
Témata: Cancellous bone - drug effects, Male, 0301 basic medicine, Cortical bone - pathology, Tibia - drug effects, Cortical bone - drug effects, Wistar, Cadmium chloride - toxicity, 01 natural sciences, Collagen Type I, 03 medical and health sciences, Cadmium Chloride, Chlorides, Cortical bone - metabolism, Cortical Bone, Chlorides - pharmacology, Animals, Zinc compounds - pharmacology, Rats, Wistar, 0105 earth and related environmental sciences, Tibia, Cancellous bone - pathology, Collagen type I - biosynthesis, Tibia - pathology, Tibia - metabolism, Dietary supplements, Procollagen - biosynthesis, Rats, Solubility, Cytoprotection, Zinc Compounds, Cancellous Bone, Dietary Supplements, Cancellous bone - metabolism, Procollagen
Popis: Cadmium (Cd) is a toxic metal that damages bone tissue by affecting its mineral and organic components. The organic matrix is mainly (90%) composed of collagen, which determines the biomechanical strength of bone. The aim of this study was to evaluate the effect of zinc (Zn) supplementation (30 or 60 mg l–1) under moderate and relatively high exposure to Cd (5 and 50 mg l–1) on collagen in the rat tibia proximal epiphysis and diaphysis (regions abundant in trabecular and cortical bone, respectively). Significant decrease in collagen type I biosynthesis was found in both regions of the tibia in Cd‐treated rats, whereas the supplementation with Zn provided significant protection against this effect. Western blot confirmed the presence of the major type I collagen in the tibia epiphysis and diaphysis, but collagen type II was revealed only in the epiphysis. Acetic acid‐ and pepsin‐soluble collagen concentration in the tibia epiphysis and diaphysis was significantly increased due to the exposure to Cd, whereas the supplementation with Zn protected, partially or totally, from these effects, depending on the used concentration. The supplementation with Zn also provided protection from unfavorable Cd impact on the maturation of the bone collagen, as the ratio of cross‐links to monomers was higher compared to the Cd‐treated group. This report confirms our previous findings on the preventive action of Zn against harmful effects of Cd on bone, but additionally, and to the best of our knowledge for the first time, explains the possible mechanism of the beneficial influence of this bioelement.
Druh dokumentu: Article
Jazyk: English
ISSN: 1099-1263
0260-437X
DOI: 10.1002/jat.3608
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/29508442
https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.3608
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802227582645825
https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.3608
https://www.ncbi.nlm.nih.gov/pubmed/29508442
https://pubmed.ncbi.nlm.nih.gov/29508442/
Rights: Wiley Online Library User Agreement
Přístupové číslo: edsair.doi.dedup.....a0f9dabbc169e3646817dd86b67754a4
Databáze: OpenAIRE
Popis
Abstrakt:Cadmium (Cd) is a toxic metal that damages bone tissue by affecting its mineral and organic components. The organic matrix is mainly (90%) composed of collagen, which determines the biomechanical strength of bone. The aim of this study was to evaluate the effect of zinc (Zn) supplementation (30 or 60 mg l–1) under moderate and relatively high exposure to Cd (5 and 50 mg l–1) on collagen in the rat tibia proximal epiphysis and diaphysis (regions abundant in trabecular and cortical bone, respectively). Significant decrease in collagen type I biosynthesis was found in both regions of the tibia in Cd‐treated rats, whereas the supplementation with Zn provided significant protection against this effect. Western blot confirmed the presence of the major type I collagen in the tibia epiphysis and diaphysis, but collagen type II was revealed only in the epiphysis. Acetic acid‐ and pepsin‐soluble collagen concentration in the tibia epiphysis and diaphysis was significantly increased due to the exposure to Cd, whereas the supplementation with Zn protected, partially or totally, from these effects, depending on the used concentration. The supplementation with Zn also provided protection from unfavorable Cd impact on the maturation of the bone collagen, as the ratio of cross‐links to monomers was higher compared to the Cd‐treated group. This report confirms our previous findings on the preventive action of Zn against harmful effects of Cd on bone, but additionally, and to the best of our knowledge for the first time, explains the possible mechanism of the beneficial influence of this bioelement.
ISSN:10991263
0260437X
DOI:10.1002/jat.3608