Challenges in using allylthiourea and chlorate as specific nitrification inhibitors
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| Title: | Challenges in using allylthiourea and chlorate as specific nitrification inhibitors |
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| Authors: | K. Tatari, A. Gülay, B. Thamdrup, H.-J. Albrechtsen, B.F. Smets |
| Source: | Tatari, K, Gülay, A, Thamdrup, B, Albrechtsen, H-J & Smets, B F 2017, ' Challenges in using allylthiourea and chlorate as specific nitrification inhibitors ', Chemosphere, vol. 182, pp. 301-305 . https://doi.org/10.1016/j.chemosphere.2017.05.005 |
| Publisher Information: | Elsevier BV, 2017. |
| Publication Year: | 2017 |
| Subject Terms: | 0301 basic medicine, Nitrite, 01 natural sciences, Thiourea/analogs & derivatives, Water Purification, 03 medical and health sciences, Chlorides, Nitrification/drug effects, Water Purification/methods, Ammonium Compounds, Drinking water, Inhibition, 0105 earth and related environmental sciences, Chlorates/chemistry, Drinking Water, Research, Thiourea, Nitrification, 6. Clean water, Chlorate, Chlorides/analysis, Chlorates, ATU, Ammonium Compounds/analysis, Ammonium, Filtration |
| Description: | Allylthiourea (ATU) and chlorate (ClO3-) are often used to selectively inhibit nitritation and nitratation. In this work we identified challenges with use of these compounds in inhibitory assays with filter material from a biological rapid sand filter for groundwater treatment. Inhibition was investigated in continuous-flow lab-scale columns, packed with filter material from a full-scale filter and supplied with NH4+ or NO2-. ATU concentrations of 0.1-0.5 mM interfered with the indophenol blue method for NH4+ quantification leading to underestimation of the measured NH4+ concentration. Interference was stronger at higher ATU levels and resulted in no NH4+ detection at 0.5 mM ATU. ClO3- at typical concentrations for inhibition assays (1-10 mM) inhibited nitratation by less than 6%, while nitritation was instead inhibited by 91% when NH4+ was supplied. On the other hand, nitratation was inhibited by 67-71% at 10-20 mM ClO3- when NO2- was supplied, suggesting significant nitratation inhibition at higher NO2- concentrations. No chlorite (ClO2-) was detected in the effluent, and thus we could not confirm that nitritation inhibition was caused by ClO3- reduction to ClO2-. In conclusion, ATU and ClO3- should be used with caution in inhibition assays, because analytical interference and poor selectivity for the targeted process may affect the experimental outcome and compromise result interpretation. |
| Document Type: | Article |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2017.05.005 |
| Access URL: | https://backend.orbit.dtu.dk/ws/files/167227937/Tatari_et_al_2_002_.pdf https://pubmed.ncbi.nlm.nih.gov/28505572 https://pubag.nal.usda.gov/catalog/5685512 https://ui.adsabs.harvard.edu/abs/2017Chmsp.182..301T/abstract https://www.ncbi.nlm.nih.gov/pubmed/28505572 https://orbit.dtu.dk/en/publications/challenges-in-using-allylthiourea-and-chlorate-as-specific-nitrif https://core.ac.uk/display/84097712 https://orbit.dtu.dk/files/167227937/Tatari_et_al_2_002_.pdf https://orbit.dtu.dk/en/publications/c6f878b2-09c7-4ac4-877f-d0b85b51654f https://portal.findresearcher.sdu.dk/da/publications/3749b2fe-ac68-4dee-9178-718b17e3e4fc https://doi.org/10.1016/j.chemosphere.2017.05.005 https://portal.findresearcher.sdu.dk/da/publications/3749b2fe-ac68-4dee-9178-718b17e3e4fc |
| Rights: | Elsevier TDM |
| Accession Number: | edsair.doi.dedup.....a084b49593b69290cdb39a086e4361f5 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Allylthiourea (ATU) and chlorate (ClO3-) are often used to selectively inhibit nitritation and nitratation. In this work we identified challenges with use of these compounds in inhibitory assays with filter material from a biological rapid sand filter for groundwater treatment. Inhibition was investigated in continuous-flow lab-scale columns, packed with filter material from a full-scale filter and supplied with NH4+ or NO2-. ATU concentrations of 0.1-0.5 mM interfered with the indophenol blue method for NH4+ quantification leading to underestimation of the measured NH4+ concentration. Interference was stronger at higher ATU levels and resulted in no NH4+ detection at 0.5 mM ATU. ClO3- at typical concentrations for inhibition assays (1-10 mM) inhibited nitratation by less than 6%, while nitritation was instead inhibited by 91% when NH4+ was supplied. On the other hand, nitratation was inhibited by 67-71% at 10-20 mM ClO3- when NO2- was supplied, suggesting significant nitratation inhibition at higher NO2- concentrations. No chlorite (ClO2-) was detected in the effluent, and thus we could not confirm that nitritation inhibition was caused by ClO3- reduction to ClO2-. In conclusion, ATU and ClO3- should be used with caution in inhibition assays, because analytical interference and poor selectivity for the targeted process may affect the experimental outcome and compromise result interpretation. |
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| ISSN: | 00456535 |
| DOI: | 10.1016/j.chemosphere.2017.05.005 |