Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen
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| Názov: | Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen |
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| Autori: | Petra Turčić, Mario Gabričević, Hrvoje Šošić, Gorana Aralica, Piotr Wardega, Ana Gudelj Gračanin, Renata Novak Kujundžić, Željko Kaštelan, Damir Babić, Paško Konjevoda, Jelena Barać Žutelija, Nikola Štambuk, Sven Seiwerth |
| Zdroj: | Int J Mol Sci International Journal of Molecular Sciences Volume 20 Issue 9 |
| Informácie o vydavateľovi: | MDPI AG, 2019. |
| Rok vydania: | 2019 |
| Predmety: | Male, 0301 basic medicine, Nanomedicine / methods, Prostate-Specific Antigen / immunology, Prostatic Neoplasms / immunology, prostate specific antigen, Article, Protein Structure, Secondary, neoplasm, biomarker, antisense peptide, immunohistochemistry, nanomedicine, 03 medical and health sciences, Peptides / immunology, Biomarkers, Tumor, Humans, 0303 health sciences, Prostatic Neoplasms, Prostate-Specific Antigen, Immunohistochemistry, Biomarkers, Tumor / immunology, 3. Good health, Interdisciplinary Natural Sciences, Nanomedicine, Peptides |
| Popis: | The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense–antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines. |
| Druh dokumentu: | Article Other literature type |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20092090 |
| Prístupová URL adresa: | https://www.mdpi.com/1422-0067/20/9/2090/pdf https://pubmed.ncbi.nlm.nih.gov/31035335 https://www.bib.irb.hr/1010802 https://doi.org/10.3390/ijms20092090 https://europepmc.org/articles/PMC6540241 https://www.bib.irb.hr/1010802 https://pubmed.ncbi.nlm.nih.gov/31035335/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540241 http://fulir.irb.hr/4862/ https://www.mdpi.com/1422-0067/20/9/2090/htm http://fulir.irb.hr/4862/1/StambukN_TargetingTumorMarkersIJMS_20_2019_02090.pdf https://urn.nsk.hr/urn:nbn:hr:105:903196 https://doi.org/10.3390/ijms20092090 http://doi.org/10.3390/ijms20092090 |
| Rights: | CC BY URL: http://rightsstatements.org/vocab/InC/1.0/ |
| Prístupové číslo: | edsair.doi.dedup.....9e9e5cd6f4bd651480be0dec8edf6eb0 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense–antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines. |
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| ISSN: | 14220067 |
| DOI: | 10.3390/ijms20092090 |