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An Experimental Model-Based Exploration of Cytokines in Ablative Radiation-Induced Lung Injury In Vivo and In Vitro

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Title: An Experimental Model-Based Exploration of Cytokines in Ablative Radiation-Induced Lung Injury In Vivo and In Vitro
Authors: Jaeho Cho, Joo Heon Yoon, Zhen Yu Hong, Kwang Hyun Song, Michael D. Story
Contributors: Zhen-Yu Hong, Kwang Hyun Song, Joo-Heon Yoon, Jaeho Cho, Michael D. Story, Yoon, Joo Heon, Cho, Jae Ho, Hong, Zhen Yu, Hong, Zhen-Yu
Source: Lung. 193:409-419
Publisher Information: Springer Science and Business Media LLC, 2015.
Publication Year: 2015
Subject Terms: Ablation Techniques, 0301 basic medicine, Time Factors, Radiation Pneumonitis/blood, Radiation Pneumonitis/genetics, Lung/metabolism, Mice, Alveolar/radiation effects, 0302 clinical medicine, Alveolar Epithelial Cells/immunology, Lung/radiation effects, Lung, Fibroblasts/metabolism, Mice, Inbred C3H, Cytokines/genetics, Fibroblasts/radiation effects, Alveolar/metabolism, Inbred C3H, 3. Good health, Lung/immunology, Radiation Pneumonitis/immunology, Cytokines, Stereotactic ablative radiotherapy, Radiation pneumonitis, Radiation Pneumonitis/etiology, Radiation fibrosis, Alveolar Epithelial Cells/radiation effects, Alveolar Epithelial Cells/metabolism, Cytokines/blood, Radiation Dosage, Radiosurgery, 03 medical and health sciences, Cytokines/immunology, Fibroblasts/immunology, Small-animal model, Macrophages, Alveolar, Alveolar/immunology, Animals, Cytokine, Animal, Macrophages, Fibroblasts, Coculture Techniques, Radiation Pneumonitis, Disease Models, Animal, Gene Expression Regulation, Alveolar Epithelial Cells, Disease Models, NIH 3T3 Cells
Description: Stereotactic ablative radiotherapy is a newly emerging radiotherapy treatment method that, compared with conventionally fractionated radiation therapy (CFRT), allows an ablative dose of radiation to be delivered to a confined area around a tumor. The aim of the present study was to investigate the changes of various cytokines that may be involved in ablative radiation-induced lung injury in vitro and in vivo.In the in vivo study, ablative-dose radiation was delivered to a small volume of the left lung of C3H/HeJCr mice using a small-animal irradiator. The levels of 24 cytokines in the peripheral blood were tested at several time points after irradiation. For the in vitro study, three mouse cell types (type II pneumocytes, alveolar macrophages, and fibroblasts) known to play important roles in radiation-induced pneumonitis and lung fibrosis were analyzed using a co-culture system.In the in vivo study, we found obvious patterns of serum cytokine changes depending on the volume of tissue irradiated (2-mm vs. 3.5-mm collimator). Only the levels of 3 cytokines increased with the 2-mm collimator at the acute phase (1-2 weeks after irradiation), while the majority of cytokines were elevated with the 3.5-mm collimator. In the in vitro co-culture system, after the cells were given an ablative dose of irradiation, the levels of five cytokines (GM-CSF, G-CSF, IL-6, MCP-1, and KC) increased significantly in a dose-dependent manner.The cytokine levels in our radiation-induced lung injury model showed specific changes, both in vivo and in vitro. These results imply that biological studies related to ablative-dose small-volume irradiation should be investigated using the corresponding experimental models rather than on those simulating large-volume CFRT.
Document Type: Article
File Description: 409~419
Language: English
ISSN: 1432-1750
0341-2040
DOI: 10.1007/s00408-015-9705-y
Access URL: https://pubmed.ncbi.nlm.nih.gov/25749666
https://utsouthwestern.pure.elsevier.com/en/publications/an-experimental-model-based-exploration-of-cytokines-in-ablative-
https://rd.springer.com/article/10.1007/s00408-015-9705-y
https://mdanderson.elsevierpure.com/en/publications/an-experimental-model-based-exploration-of-cytokines-in-ablative-
https://pubmed.ncbi.nlm.nih.gov/25749666/
https://link.springer.com/article/10.1007/s00408-015-9705-y
https://www.ncbi.nlm.nih.gov/pubmed/25749666
Rights: Springer TDM
CC BY NC ND
Accession Number: edsair.doi.dedup.....9ac6b7f817f74d008f9e039d2aef7aa3
Database: OpenAIRE
Description
Abstract:Stereotactic ablative radiotherapy is a newly emerging radiotherapy treatment method that, compared with conventionally fractionated radiation therapy (CFRT), allows an ablative dose of radiation to be delivered to a confined area around a tumor. The aim of the present study was to investigate the changes of various cytokines that may be involved in ablative radiation-induced lung injury in vitro and in vivo.In the in vivo study, ablative-dose radiation was delivered to a small volume of the left lung of C3H/HeJCr mice using a small-animal irradiator. The levels of 24 cytokines in the peripheral blood were tested at several time points after irradiation. For the in vitro study, three mouse cell types (type II pneumocytes, alveolar macrophages, and fibroblasts) known to play important roles in radiation-induced pneumonitis and lung fibrosis were analyzed using a co-culture system.In the in vivo study, we found obvious patterns of serum cytokine changes depending on the volume of tissue irradiated (2-mm vs. 3.5-mm collimator). Only the levels of 3 cytokines increased with the 2-mm collimator at the acute phase (1-2 weeks after irradiation), while the majority of cytokines were elevated with the 3.5-mm collimator. In the in vitro co-culture system, after the cells were given an ablative dose of irradiation, the levels of five cytokines (GM-CSF, G-CSF, IL-6, MCP-1, and KC) increased significantly in a dose-dependent manner.The cytokine levels in our radiation-induced lung injury model showed specific changes, both in vivo and in vitro. These results imply that biological studies related to ablative-dose small-volume irradiation should be investigated using the corresponding experimental models rather than on those simulating large-volume CFRT.
ISSN:14321750
03412040
DOI:10.1007/s00408-015-9705-y