MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma
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| Titel: | MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma |
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| Autoren: | Woo Jin Hyung, Da Hye Lee, Se Hoon Kim, Ji Eun Kim, Yang Ki Minn, Junjeong Choi, Seung Ho Yang, Ho Taek Song, Beom Jin Lim |
| Weitere Verfasser: | Yang-Ki Minn, Da Hye Lee, Woo Jin Hyung, Ji Eun Kim, Junjeong Choi, Seung-Ho Yang, Hotaek Song, Beom Jin Lim, Se Hoon Kim, Kim, Se Hoon, Song, Ho Taek, Lee, Da Hye, Lim, Beom Jin, Hyung, Woo Jin |
| Quelle: | International Journal of Oncology. 45:2403-2410 |
| Verlagsinformationen: | Spandidos Publications, 2014. |
| Publikationsjahr: | 2014 |
| Schlagwörter: | Male, 0301 basic medicine, Brain Neoplasms/genetics, Adenocarcinoma, Cell Proliferation/genetics, Mice, 03 medical and health sciences, 0302 clinical medicine, Adenocarcinoma/genetics, Stomach Neoplasms, Repressor Proteins/biosynthesis, Animals, Humans, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Zinc Finger E-box Binding Homeobox 2, Homeodomain Proteins, Neoplastic, Repressor Proteins/genetics, Adenocarcinoma/pathology, Brain Neoplasms, Stomach Neoplasms/pathology, Homeodomain Proteins/biosynthesis, Middle Aged, 3. Good health, Gene Expression Regulation, Neoplastic, Repressor Proteins, MicroRNAs/genetics, Stomach Neoplasms/genetics, MicroRNAs, Gene Expression Regulation, Lymphatic Metastasis, Brain Neoplasms/pathology, Brain Neoplasms/secondary, Female, Homeodomain Proteins/genetics, MicroRNAs/biosynthesis |
| Beschreibung: | Although the incidence of brain metastasis in gastric cancer is relatively low, its prevalence may increase with improved therapy and longer survival tumors. The molecular mechanisms underlying brain metastases are not well understood. To gain insight into the mechanism of brain metastasis, we studied differences in microRNA (miRNA) expression levels in 8 cases of matched primary gastric adenocarcinoma and brain metastatic adenocarcinoma using the Illumina microRNA microarray chip. We identified 6 upregulated and 2 downregulated miRNAs in all 8 cases simultaneously. Interestingly, 2 out of 8 miRNAs (hsa-miR‑141-3p and hsa-miR-200b-3p) belonged to the miR-200 family. Online microRNA database searching revealed that ZEB2 is the top-ranked target gene for hsa-miR141-3p and hsa-miR-200b-3p, prompting us to focus ZEB2 expression in brain metastatic adenocarcinoma. We confirmed that ZEB2 expression was markedly downregulated in some brain metastatic samples. In addition, decreased ZEB2 expression was noted by western blot analysis of 2 metastatic gastric adenocarcinoma cell types that were derived by in vivo selection following intracardiac injection of gastric cancer cell lines. In conclusion, we demonstrate that expression of miRNA-200 family members and ZEB2 are associated with brain metastases of gastric adenocarcinoma, not only in matched patient samples, but also in metastatic cell lines that were derived by in vivo selection. |
| Publikationsart: | Article |
| Dateibeschreibung: | 2403~2410 |
| Sprache: | English |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2014.2680 |
| Zugangs-URL: | https://www.spandidos-publications.com/10.3892/ijo.2014.2680/download https://pubmed.ncbi.nlm.nih.gov/25270520 https://pubmed.ncbi.nlm.nih.gov/25270520/ https://www.spandidos-publications.com/10.3892/ijo.2014.2680/download https://www.spandidos-publications.com/ijo/45/6/2403/download https://yonsei.pure.elsevier.com/en/publications/microrna-200-family-members-and-zeb2-are-associated-with-brain-me https://mdanderson.elsevierpure.com/en/publications/microrna-200-family-members-and-zeb2-are-associated-with-brain-me https://ir.ymlib.yonsei.ac.kr/handle/22282913/99873 |
| Rights: | CC BY NC ND |
| Dokumentencode: | edsair.doi.dedup.....9a408ce2fd0410370d71af444e49ec1a |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Although the incidence of brain metastasis in gastric cancer is relatively low, its prevalence may increase with improved therapy and longer survival tumors. The molecular mechanisms underlying brain metastases are not well understood. To gain insight into the mechanism of brain metastasis, we studied differences in microRNA (miRNA) expression levels in 8 cases of matched primary gastric adenocarcinoma and brain metastatic adenocarcinoma using the Illumina microRNA microarray chip. We identified 6 upregulated and 2 downregulated miRNAs in all 8 cases simultaneously. Interestingly, 2 out of 8 miRNAs (hsa-miR‑141-3p and hsa-miR-200b-3p) belonged to the miR-200 family. Online microRNA database searching revealed that ZEB2 is the top-ranked target gene for hsa-miR141-3p and hsa-miR-200b-3p, prompting us to focus ZEB2 expression in brain metastatic adenocarcinoma. We confirmed that ZEB2 expression was markedly downregulated in some brain metastatic samples. In addition, decreased ZEB2 expression was noted by western blot analysis of 2 metastatic gastric adenocarcinoma cell types that were derived by in vivo selection following intracardiac injection of gastric cancer cell lines. In conclusion, we demonstrate that expression of miRNA-200 family members and ZEB2 are associated with brain metastases of gastric adenocarcinoma, not only in matched patient samples, but also in metastatic cell lines that were derived by in vivo selection. |
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| ISSN: | 17912423 10196439 |
| DOI: | 10.3892/ijo.2014.2680 |