Zobraziť v EDS

Potentials of a Plasma-Aerosol System for Wound Healing Advanced by Drug Introduction: An In Vitro Study

Uložené v:
Podrobná bibliografia
Názov: Potentials of a Plasma-Aerosol System for Wound Healing Advanced by Drug Introduction: An In Vitro Study
Autori: Sremački, Ivana, Asadian, Mahtab, De Geyter, Nathalie, Leys, Christophe, Geris, Liesbet, Nikiforov, Anton
Prispievatelia: FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen
Zdroj: ACS Biomaterials Science & Engineering. 9:2392-2407
Informácie o vydavateľovi: American Chemical Society (ACS), 2023.
Rok vydania: 2023
Predmety: 0301 basic medicine, Biomedical fields, 0206 medical engineering, Biomedical Engineering, wound healing, 02 engineering and technology, cold plasma, Aerosols/pharmacology, Ingénierie, informatique & technologie, Biomaterials, In-vitro, 03 medical and health sciences, Reactive Oxygen Species/metabolism, Plasma treatment, FTIC dextran, Vitro studies, Aerosol systems, Reactive Nitrogen Species/pharmacology, Collagen/pharmacology, Aerosols, Wound Healing, Hydrogen Peroxide, Reactive Nitrogen Species, Biomedical applications, Engineering, computing & technology, 3. Good health, drug delivery, Cold plasmas, biomedical application, Collagen, Reactive Oxygen Species, Hydrogen Peroxide/pharmacology
Popis: Cold plasmas have found their application in a wide range of biomedical fields by virtue of their high chemical reactivity. In the past decades, many attempts have been made to use cold plasmas in wound healing, and within this field, many studies have focused on plasma-induced cell proliferation mechanisms. In this work, one step further has been taken to demonstrate the advanced role of plasma in wound healing. To this end, the simultaneous ability of plasma to induce cell proliferation and permeabilize treated cells has been examined in the current study. The driving force was to advance the wound healing effect of plasma with drug delivery. On this subject, we demonstrate in vitro the healing effect of Ar, Ar+N2 plasma, and their aerosol counterparts. A systematic study has been carried out to study the role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cell adhesion, signaling, differentiation, and proliferation. An additional investigation was also performed to study the permeabilization of cells and the delivery of the modeled drug carrier fluorescein isothiocyanate (FITC) labeled dextran into cells upon plasma treatment. Short 35 s plasma treatments were found to promote fibroblast adhesion, migration, signaling, proliferation, and differentiation by means of reactive oxygen and nitrogen species (RONS) created by plasma and deposited into the cell environment. The impact of the plasma downstream products NO2- and NO3- on the expressions of the focal adhesion's genes, syndecans, and collagens was observed to be prominent. On the other hand, the differentiation of fibroblasts to myofibroblasts was mainly initiated by ROS produced by the plasma. In addition, the ability of plasma to locally permeabilize fibroblast cells was demonstrated. During proliferative cell treatment, plasma can simultaneously induce cell membrane permeabilization (d ∼ 7.3 nm) by the species OH and H2O2. The choice for a plasma or a plasma-aerosol configuration thus allows the possibility to change the spatial chemistry of drug delivery molecules and thus to locally deliver drugs. Accordingly, this study offers a pivotal step toward plasma-assisted wound healing advanced by drug delivery.
Druh dokumentu: Article
Jazyk: English
ISSN: 2373-9878
DOI: 10.1021/acsbiomaterials.2c01391
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/37129346
Rights: STM Policy #29
Prístupové číslo: edsair.doi.dedup.....963d0170e50c628e16f3094321bf472b
Databáza: OpenAIRE
Popis
Abstrakt:Cold plasmas have found their application in a wide range of biomedical fields by virtue of their high chemical reactivity. In the past decades, many attempts have been made to use cold plasmas in wound healing, and within this field, many studies have focused on plasma-induced cell proliferation mechanisms. In this work, one step further has been taken to demonstrate the advanced role of plasma in wound healing. To this end, the simultaneous ability of plasma to induce cell proliferation and permeabilize treated cells has been examined in the current study. The driving force was to advance the wound healing effect of plasma with drug delivery. On this subject, we demonstrate in vitro the healing effect of Ar, Ar+N2 plasma, and their aerosol counterparts. A systematic study has been carried out to study the role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cell adhesion, signaling, differentiation, and proliferation. An additional investigation was also performed to study the permeabilization of cells and the delivery of the modeled drug carrier fluorescein isothiocyanate (FITC) labeled dextran into cells upon plasma treatment. Short 35 s plasma treatments were found to promote fibroblast adhesion, migration, signaling, proliferation, and differentiation by means of reactive oxygen and nitrogen species (RONS) created by plasma and deposited into the cell environment. The impact of the plasma downstream products NO2- and NO3- on the expressions of the focal adhesion's genes, syndecans, and collagens was observed to be prominent. On the other hand, the differentiation of fibroblasts to myofibroblasts was mainly initiated by ROS produced by the plasma. In addition, the ability of plasma to locally permeabilize fibroblast cells was demonstrated. During proliferative cell treatment, plasma can simultaneously induce cell membrane permeabilization (d ∼ 7.3 nm) by the species OH and H2O2. The choice for a plasma or a plasma-aerosol configuration thus allows the possibility to change the spatial chemistry of drug delivery molecules and thus to locally deliver drugs. Accordingly, this study offers a pivotal step toward plasma-assisted wound healing advanced by drug delivery.
ISSN:23739878
DOI:10.1021/acsbiomaterials.2c01391