AFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extract
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| Titel: | AFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extract |
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| Autoren: | Feihu Zhao, Hongmei Xu, Zuobin Wang, Wenxiao Zhang, Jingmei Li, Yingmin Qu, Zhengxun Song, Jinyun Liu |
| Weitere Verfasser: | Changchun University of Science and Technology, Eindhoven University of Technology, University of Bedfordshire |
| Quelle: | Micron. 104:1-7 |
| Verlagsinformationen: | Elsevier BV, 2018. |
| Publikationsjahr: | 2018 |
| Schlagwörter: | 0301 basic medicine, Carcinoma, Hepatocellular, cellular stiffness, Atomic force microscope (AFM), Panax, Antineoplastic Agents, Apoptosis, SMMC-7721 cells, SDG 3 – Goede gezondheid en welzijn, Microscopy, Atomic Force, Plant Roots, Cell Line, 03 medical and health sciences, hepatocelluar carcinoma, SDG 3 - Good Health and Well-being, Cell Line, Tumor, atomic force microscope, Cultured/drug effects, Tumor Cells, Cultured, Humans, Plant Extracts/isolation & purification, ginseng-extracted compounds, Plant Roots/chemistry, Microscopy, 0303 health sciences, atomic force microscopy, Tumor, cell apoptosis, Plant Extracts, American ginseng, Carcinoma, Liver Neoplasms, C130 Cell Biology, Cellular stiffness, Atomic Force, Hepatocellular, Tumor Cells, 3. Good health, Antineoplastic Agents/isolation & purification, Hepatocytes, Cell apoptosis, Panax/chemistry, Hepatocytes/drug effects |
| Beschreibung: | American ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE). The results showed that the cells treated with AGRWE exhibited significantly larger surface roughness, height and elastic modulus values than control group. Moreover, those parameters were upregulated under the higher concentration of AGRWE and longer culture time. Consequently, it indicates that the mechanical and morphological properties can be used as the apoptotic characteristics of SMMC-7721 cells. Also, the increased surface roughness and elastic modulus of cells under the AGRWE treatment have shown that the apoptosis of SMMC-7721 cells can be enhanced by AGRWE. This will provide an important implication for hepatocelluar carcinoma treatment and drug development. |
| Publikationsart: | Article |
| Sprache: | English |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2017.10.003 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/29049926 https://www.sciencedirect.com/science/article/pii/S0968432817303256 https://europepmc.org/abstract/MED/29049926 https://www.narcis.nl/publication/RecordID/oai%3Apure.tue.nl%3Apublications%2F828a6131-2e88-4702-b0d0-6fd110a9185d https://cronfa.swan.ac.uk/Record/cronfa51683 https://research.tue.nl/en/publications/afm-detected-apoptosis-of-hepatocellular-carcinoma-cells-induced- https://pubmed.ncbi.nlm.nih.gov/29049926/ https://cronfa.swan.ac.uk/Record/cronfa51683 |
| Rights: | Elsevier TDM |
| Dokumentencode: | edsair.doi.dedup.....95d1ac677fa211789b4eb80b5b9a8338 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | American ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE). The results showed that the cells treated with AGRWE exhibited significantly larger surface roughness, height and elastic modulus values than control group. Moreover, those parameters were upregulated under the higher concentration of AGRWE and longer culture time. Consequently, it indicates that the mechanical and morphological properties can be used as the apoptotic characteristics of SMMC-7721 cells. Also, the increased surface roughness and elastic modulus of cells under the AGRWE treatment have shown that the apoptosis of SMMC-7721 cells can be enhanced by AGRWE. This will provide an important implication for hepatocelluar carcinoma treatment and drug development. |
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| ISSN: | 09684328 |
| DOI: | 10.1016/j.micron.2017.10.003 |