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Differential immune response of adipocytes to virulent and attenuated Mycobacterium tuberculosis

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Titel: Differential immune response of adipocytes to virulent and attenuated Mycobacterium tuberculosis
Autoren: Woo-Sik Kim, Eui Hong Byun, Ki Nam Min, Minho Shong, Jong Seok Kim, Hwa-Jung Kim, Sung Jae Shin, Min Jeong Ryu, Jake Whang
Weitere Verfasser: Jong-Seok Kim, Min-Jeong Ryu, Eui-Hong Byun, Woo Sik Kim, Jake Whang, Ki-Nam Min, Minho Shong, Hwa-Jung Kim, Sung Jae Shin, Kim, Jong Seok, Byun, Eui Hong, Shin, Sung Jae
Quelle: Microbes and Infection. 13:1242-1251
Verlagsinformationen: Elsevier BV, 2011.
Publikationsjahr: 2011
Schlagwörter: 0301 basic medicine, Hot Temperature, Interleukin-12 Subunit p40/immunology, Mice, Interleukin-4/immunology, Adipocytes, Extracellular Space/chemistry, Adipocytes/microbiology, Interleukin-17/biosynthesis, Microscopy, 0303 health sciences, Adipocyte, Blotting, Interleukin-12 Subunit p40, Interleukin-17, Interleukin-10/biosynthesis, Interleukin-12 Subunit p40/biosynthesis, Interleukin-10, 3. Good health, L-Lactate Dehydrogenase/analysis, Tuberculosis/immunology, Western, Blotting, Western, Mycobacterium tuberculosis/growth & development, Tumor Necrosis Factor-alpha/biosynthesis, Immune Evasion, Fluorescence, 03 medical and health sciences, 3T3-L1 Cells, Formaldehyde, L-Lactate Dehydrogenase/metabolism, Mycobacterium tuberculosis/pathogenicity, Animals, Humans, Tuberculosis, Tuberculosis/microbiology, Cytokine, L-Lactate Dehydrogenase, Interleukin-6, Tumor Necrosis Factor-alpha, Interleukin-4/biosynthesis, Nitric oxide, Mycobacterium tuberculosis, Tumor Necrosis Factor-alpha/immunology, Interleukin-6/biosynthesis, Interleukin-17/immunology, Mycobacterium tuberculosis/immunology, Microscopy, Fluorescence, Adipocytes/immunology, Interleukin-6/immunology, Interleukin-4, Interleukin-10/immunology, Extracellular Space
Beschreibung: Mycobacterium tuberculosis (M. tb) takes advantage of various cell types, allowing it to remain in the host for long periods. Because adipocytes have been proposed as niches for dormant M. tb in the latent state, understanding the interaction of virulent M. tb with adipocytes is important. We compared changes in cytokine secretion from 3T3-L1 murine adipocytes infected with virulent M. tb H37Rv (V-M. tb) and attenuated M. tb H37Ra (A-M. tb) strains. Both strains maintained non-replicating states within adipocytes until 10 days post-infection. Adipocytes infected with V-M. tb secreted lower levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-12p40, IL-6, and IL-17, and lower levels of nitric oxide than those infected with A-M. tb. In contrast, the anti-inflammatory cytokines, IL-10 and IL-4, were markedly induced in V-M. tb-infected adipocytes versus those infected with A-M. tb at an early time point. Heat-killed or formalin-fixed bacteria induced lower levels of cytokines and no difference was observed between strains. Moreover, V-M. tb induced a high level of necrosis versus A-M. tb in conjunction with increased levels of LHD. These results suggest that V-M. tb regulates cytokine expression in its favor, increasing cytokines necessary for immune evasion and decreasing those required for protective immunity.
Publikationsart: Article
Dateibeschreibung: 1242~1251
Sprache: English
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2011.07.002
Zugangs-URL: https://pubmed.ncbi.nlm.nih.gov/21813088
https://yonsei.pure.elsevier.com/en/publications/differential-immune-response-of-adipocytes-to-virulent-and-attenu
http://www.sciencedirect.com/science/article/pii/S1286457911001870
https://www.sciencedirect.com/science/article/pii/S1286457911001870
Rights: Elsevier TDM
CC BY NC ND
Dokumentencode: edsair.doi.dedup.....92f1595c8acccf227f97db7b7edca80e
Datenbank: OpenAIRE
Beschreibung
Abstract:Mycobacterium tuberculosis (M. tb) takes advantage of various cell types, allowing it to remain in the host for long periods. Because adipocytes have been proposed as niches for dormant M. tb in the latent state, understanding the interaction of virulent M. tb with adipocytes is important. We compared changes in cytokine secretion from 3T3-L1 murine adipocytes infected with virulent M. tb H37Rv (V-M. tb) and attenuated M. tb H37Ra (A-M. tb) strains. Both strains maintained non-replicating states within adipocytes until 10 days post-infection. Adipocytes infected with V-M. tb secreted lower levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-12p40, IL-6, and IL-17, and lower levels of nitric oxide than those infected with A-M. tb. In contrast, the anti-inflammatory cytokines, IL-10 and IL-4, were markedly induced in V-M. tb-infected adipocytes versus those infected with A-M. tb at an early time point. Heat-killed or formalin-fixed bacteria induced lower levels of cytokines and no difference was observed between strains. Moreover, V-M. tb induced a high level of necrosis versus A-M. tb in conjunction with increased levels of LHD. These results suggest that V-M. tb regulates cytokine expression in its favor, increasing cytokines necessary for immune evasion and decreasing those required for protective immunity.
ISSN:12864579
DOI:10.1016/j.micinf.2011.07.002