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Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells

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Podrobná bibliografia
Názov: Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells
Autori: Sarah McCarrick, Mathilde N. Delaval, Ulrike M. Dauter, Annette M. Krais, Anastasiia Snigireva, Asmamaw Abera, Karin Broberg, Axel C. Eriksson, Christina Isaxon, Anda R. Gliga
Zdroj: Arch Toxicol
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2024.
Rok vydania: 2024
Predmety: 0301 basic medicine, Air Pollutants, Macrophages, 01 natural sciences, Organ Toxicity and Mechanisms, 03 medical and health sciences, Female [MeSH], Macrophages [MeSH], Charcoal [MeSH], Genotoxicity, Polycyclic aromatic hydrocarbons (PAH), Biomass [MeSH], Humans [MeSH], Air Pollutants/toxicity [MeSH], Particulate Matter/analysis [MeSH], Combustion-derived particles, Animals [MeSH], Cytokines, Polycyclic Aromatic Hydrocarbons/toxicity [MeSH], Solid biomass fuel, Particle Size [MeSH], Particulate Matter/toxicity [MeSH], Toxicity, Air Pollutants/analysis [MeSH], Cattle [MeSH], Polycyclic Aromatic Hydrocarbons/analysis [MeSH], Charcoal, Animals, Humans, Female, Cattle, Particulate Matter, Biomass, Particle Size, Polycyclic Aromatic Hydrocarbons, 0105 earth and related environmental sciences
Popis: The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC–MS). BEAS-2B bronchial epithelial cells and THP-1 derived macrophages were exposed to particle suspensions (1–150 µg/mL) for 24 h. All particles induced concentration-dependent genotoxicity (comet assay) but no pro-inflammatory cytokine release in epithelial cells, whereas dung and wood particles also induced concentration-dependent cytotoxicity (Alamar Blue). Only wood particles induced concentration-dependent cytotoxicity and genotoxicity in macrophage-like cells, while dung particles were unique at increasing secretion of pro-inflammatory cytokines (IL-6, IL-8, TNF-α). In summary, particles derived from combustion of less energy dense fuels like dung and wood had a higher PAH content and were more cytotoxic in epithelial cells. In addition, the least energy dense and cheapest fuel, dung, also induced pro-inflammatory effects in macrophage-like cells. These findings highlight the influence of fuel type on the toxic profile of the emitted particles and warrant further research to understand and mitigate health effects of indoor air pollution.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 1432-0738
0340-5761
DOI: 10.1007/s00204-024-03692-8
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/38427118
https://repository.publisso.de/resource/frl:6507558
Rights: CC BY
Prístupové číslo: edsair.doi.dedup.....926e911898701e11bff257d63eda4dbf
Databáza: OpenAIRE
Popis
Abstrakt:The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC–MS). BEAS-2B bronchial epithelial cells and THP-1 derived macrophages were exposed to particle suspensions (1–150 µg/mL) for 24 h. All particles induced concentration-dependent genotoxicity (comet assay) but no pro-inflammatory cytokine release in epithelial cells, whereas dung and wood particles also induced concentration-dependent cytotoxicity (Alamar Blue). Only wood particles induced concentration-dependent cytotoxicity and genotoxicity in macrophage-like cells, while dung particles were unique at increasing secretion of pro-inflammatory cytokines (IL-6, IL-8, TNF-α). In summary, particles derived from combustion of less energy dense fuels like dung and wood had a higher PAH content and were more cytotoxic in epithelial cells. In addition, the least energy dense and cheapest fuel, dung, also induced pro-inflammatory effects in macrophage-like cells. These findings highlight the influence of fuel type on the toxic profile of the emitted particles and warrant further research to understand and mitigate health effects of indoor air pollution.
ISSN:14320738
03405761
DOI:10.1007/s00204-024-03692-8