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In vitro antioxidant and anti-inflammatory activities of extracts from Potentilla recta and its main ellagitannin, agrimoniin

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Název: In vitro antioxidant and anti-inflammatory activities of extracts from Potentilla recta and its main ellagitannin, agrimoniin
Autoři: Agnieszka Filipek, Jakub P. Piwowarski, Michał Tomczyk, Agnieszka Bazylko, Jessica Bonarewicz
Zdroj: Journal of Ethnopharmacology. 149:222-227
Informace o vydavateli: Elsevier BV, 2013.
Rok vydání: 2013
Témata: thin layer, 0301 basic medicine, Free Radicals, aerial - chemistry, Lipoxygenase, Anti-Inflammatory Agents, Hyaluronoglucosaminidase, Antioxidants, Antioxidants - isolation & purification, 03 medical and health sciences, Potentilla - chemistry, Lipoxygenase - chemistry, Anti-inflammatory agents - pharmacology, Plant extracts - isolation & purification, Chromatography, Hydrolyzable tannins - pharmacology, 0303 health sciences, Antioxidants - pharmacology, Plant Extracts, Plant extracts - pharmacology, Hydrogen peroxide - chemistry, Plant components, Anti-inflammatory agents - isolation & purification, Hydrogen Peroxide, Plant Components, Aerial, Free radicals - chemistry, Hyaluronoglucosaminidase - antagonists & inhibitors, Hydrolyzable Tannins, 3. Good health, Ethnopharmacology, Potentilla, Hydrolyzable tannins - isolation & purification, Chromatography, Thin Layer
Popis: Potentilla recta is one of the numerous cinquefoil species growing in Poland. It is used in traditional medicine e.g. in the treatment of skin inflammation.The purpose of the present study is to evaluate antioxidant and anti-inflammatory activities of extracts and subfractions of the P. recta herb (obtained by using solvents of different polarity) in in vitro systems as well as to examine their chemical composition.Antioxidant activities of the extracts, subfractions and agrimoniin were evaluated using DPPH and three other radicals (O2(-), H2O2, and HClO) generated in cell-free systems. Anti-hyaluronidase activity was measured by using the turbidimetric method. Inhibition of lipoxidase activity was measured spectrophotometrically, using linoleic acid as a substrate. The composition of the most active subfraction was determined using the HPLC-DAD-MS(n) method.All tested samples showed scavenging activity against all the examined reactive species in a concentration-dependent manner. The highest scavenging activity against DPPH, H2O2 and HClO was observed in the ethyl acetate subfraction (PRE3) (SC50 ± SEM [μg/mL]: 25.39 ± 2.49, 1.79 ± 0.25 and 8.52 ± 1.16 respectively). It was only in the xanthine/xanthine oxidase system that the antioxidation potential of the diethyl ether subfraction (PRE2) (SC50 ± SEM [μg/mL]: 6.59 ± 1.33) was higher than that of the subfraction PRE3 (SC50 ± SEM [μg/mL]: 8.57 ± 1.37). Also, in the studies of lipoxidase and hyaluronidase inhibition activity the strongest effect was observed for PRE3, with IC50 [μg/mL] = 86.31 ± 5.46, and 12.99 ± 1.31, respectively. The chromatographic method (HPTLC-DPPH) revealed that the principal substance responsible for the activity, is a tannin like compound. Isolated agrimoniin showed significant reactive oxygen species scavenging activity and significant enzyme inhibition activity (including xanthine oxidase inhibition activity). Agrimoniin exerted the strongest scavenging activity against H2O2 (SC50 ± SEM [μM]: 0.20 ± 0.01). This compound also significantly inhibited the enzymatic activity of lipoxidase (IC50 [μM] = 36.47 ± 1.29), and, particularly, of hyaluronidase (IC50 [μM] = 2.65 ± 0.40).The strong scavenging activity against H2O2, and the inhibition of the enzymatic activity of lipoxidase, and particularly, hyaluronidase observed for the tested subfractions and agrimoniin, partly explain the beneficial effects of P. recta in treatment of skin inflammation.
Druh dokumentu: Article
Jazyk: English
ISSN: 0378-8741
DOI: 10.1016/j.jep.2013.06.026
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/23811215
https://europepmc.org/article/MED/23811215
https://www.sciencedirect.com/science/article/pii/S0378874113004467
https://pubmed.ncbi.nlm.nih.gov/23811215/
https://www.ncbi.nlm.nih.gov/pubmed/23811215
Rights: Elsevier TDM
Přístupové číslo: edsair.doi.dedup.....8f3837154da1615c8604d4604c7156a3
Databáze: OpenAIRE
Popis
Abstrakt:Potentilla recta is one of the numerous cinquefoil species growing in Poland. It is used in traditional medicine e.g. in the treatment of skin inflammation.The purpose of the present study is to evaluate antioxidant and anti-inflammatory activities of extracts and subfractions of the P. recta herb (obtained by using solvents of different polarity) in in vitro systems as well as to examine their chemical composition.Antioxidant activities of the extracts, subfractions and agrimoniin were evaluated using DPPH and three other radicals (O2(-), H2O2, and HClO) generated in cell-free systems. Anti-hyaluronidase activity was measured by using the turbidimetric method. Inhibition of lipoxidase activity was measured spectrophotometrically, using linoleic acid as a substrate. The composition of the most active subfraction was determined using the HPLC-DAD-MS(n) method.All tested samples showed scavenging activity against all the examined reactive species in a concentration-dependent manner. The highest scavenging activity against DPPH, H2O2 and HClO was observed in the ethyl acetate subfraction (PRE3) (SC50 ± SEM [μg/mL]: 25.39 ± 2.49, 1.79 ± 0.25 and 8.52 ± 1.16 respectively). It was only in the xanthine/xanthine oxidase system that the antioxidation potential of the diethyl ether subfraction (PRE2) (SC50 ± SEM [μg/mL]: 6.59 ± 1.33) was higher than that of the subfraction PRE3 (SC50 ± SEM [μg/mL]: 8.57 ± 1.37). Also, in the studies of lipoxidase and hyaluronidase inhibition activity the strongest effect was observed for PRE3, with IC50 [μg/mL] = 86.31 ± 5.46, and 12.99 ± 1.31, respectively. The chromatographic method (HPTLC-DPPH) revealed that the principal substance responsible for the activity, is a tannin like compound. Isolated agrimoniin showed significant reactive oxygen species scavenging activity and significant enzyme inhibition activity (including xanthine oxidase inhibition activity). Agrimoniin exerted the strongest scavenging activity against H2O2 (SC50 ± SEM [μM]: 0.20 ± 0.01). This compound also significantly inhibited the enzymatic activity of lipoxidase (IC50 [μM] = 36.47 ± 1.29), and, particularly, of hyaluronidase (IC50 [μM] = 2.65 ± 0.40).The strong scavenging activity against H2O2, and the inhibition of the enzymatic activity of lipoxidase, and particularly, hyaluronidase observed for the tested subfractions and agrimoniin, partly explain the beneficial effects of P. recta in treatment of skin inflammation.
ISSN:03788741
DOI:10.1016/j.jep.2013.06.026