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Reduced ATP turnover during hibernation in relaxed skeletal muscle

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Titel: Reduced ATP turnover during hibernation in relaxed skeletal muscle
Autoren: de Napoli, Cosimo, Schmidt, Luisa, Montesel, Mauro, Cussonneau, Laura, Sanniti, Samuele, Marcucci, Lorenzo, Germinario, Elena, Kindberg, Jonas, Evans, Alina Lynn, Gauquelin-Koch, Guillemette, Narici, Marco, Bertile, Fabrice, Lefai, Etienne, Krüger, Marcus, Nogara, Leonardo, Blaauw, Bert
Weitere Verfasser: ROSSI, Sabine, Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)
Quelle: Nat Commun
Nature Communications, Vol 16, Iss 1, Pp 1-14 (2025)
Nature Communications
80(2025)
Verlagsinformationen: Springer Science and Business Media LLC, 2025.
Publikationsjahr: 2025
Schlagwörter: Male, Proteomics, Adenosine Triphosphatases, Brown bears hibernation, Myosin Light Chains, Proteome, [SDV]Life Sciences [q-bio], Science, VDP::Zoologiske og botaniske fag: 480, Muscle Fibers, Skeletal, brown bear, Myosins, ATPase activity, Article, [SDV] Life Sciences [q-bio], Adenosine Triphosphate, Hibernation, VDP::Zoology and botany: 480, Animals, Phosphorylation, Muscle, Skeletal, Ursidae
Beschreibung: Hibernating brown bears, due to a drastic reduction in metabolic rate, show only moderate muscle wasting. Here, we evaluate if ATPase activity of resting skeletal muscle myosin can contribute to this energy sparing. By analyzing single muscle fibers taken from the same bears, either during hibernation or in summer, we find that fibers from hibernating bears have a mild decline in force production and a significant reduction in ATPase activity. Single fiber proteomics, western blotting, and immunohistochemical analyses reveal major remodeling of the mitochondrial proteome during hibernation. Furthermore, using bioinformatical approaches and western blotting we find that phosphorylated myosin light chain, a known stimulator of basal myosin ATPase activity, is decreased in hibernating and disused muscles. These results suggest that skeletal muscle limits energy loss by reducing myosin ATPase activity, indicating a possible role for myosin ATPase activity modulation in multiple muscle wasting conditions.
Publikationsart: Article
Other literature type
Dateibeschreibung: application/pdf
Sprache: English
ISSN: 2041-1723
DOI: 10.1038/s41467-024-55565-4
Zugangs-URL: https://pubmed.ncbi.nlm.nih.gov/39747078
https://doaj.org/article/29cd158edce54e0d8a65f14757656ee0
https://hdl.handle.net/11250/3197778
https://hal.inrae.fr/hal-04892800v1/document
https://hal.inrae.fr/hal-04892800v1
https://doi.org/10.1038/s41467-024-55565-4
https://hdl.handle.net/11250/3174346
Rights: CC BY
Dokumentencode: edsair.doi.dedup.....8bd0c02c4c4de164dfb9e9d5a3be60e0
Datenbank: OpenAIRE
Beschreibung
Abstract:Hibernating brown bears, due to a drastic reduction in metabolic rate, show only moderate muscle wasting. Here, we evaluate if ATPase activity of resting skeletal muscle myosin can contribute to this energy sparing. By analyzing single muscle fibers taken from the same bears, either during hibernation or in summer, we find that fibers from hibernating bears have a mild decline in force production and a significant reduction in ATPase activity. Single fiber proteomics, western blotting, and immunohistochemical analyses reveal major remodeling of the mitochondrial proteome during hibernation. Furthermore, using bioinformatical approaches and western blotting we find that phosphorylated myosin light chain, a known stimulator of basal myosin ATPase activity, is decreased in hibernating and disused muscles. These results suggest that skeletal muscle limits energy loss by reducing myosin ATPase activity, indicating a possible role for myosin ATPase activity modulation in multiple muscle wasting conditions.
ISSN:20411723
DOI:10.1038/s41467-024-55565-4