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Balancing Scaffold Degradation and Neo-Tissue Formation in In Situ Tissue Engineered Vascular Grafts

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Title: Balancing Scaffold Degradation and Neo-Tissue Formation in In Situ Tissue Engineered Vascular Grafts
Authors: Marcelle Uiterwijk, Bram F. Coolen, Jan-Willem van Rijswijk, Serge H.M. Söntjens, Michel H.C.J. van Houtem, Wojciech Szymczyk, Laura Rijns, Henk M. Janssen, Allard van de Wal, Bas A.J.M. de Mol, Carlijn V.C. Bouten, Gustav J. Strijkers, Patricia Y.W. Dankers, Jolanda Kluin
Source: Tissue Engineering Part A. 30:421-436
Publisher Information: Mary Ann Liebert Inc, 2024.
Publication Year: 2024
Subject Terms: Male, Tissue Scaffolds, Tissue Engineering, in situ tissue engineering, Biocompatible Materials, scaffold degradation, Blood Vessel Prosthesis, Rats, Tissue Scaffolds/chemistry, sequence-controlled biomaterials, Tissue Engineering/methods, Animals, small vascular grafts, Abdominal, Aorta, Abdominal, Aorta, MRI, Biocompatible Materials/chemistry
Description: An essential aspect of cardiovascular in situ tissue engineering (TE) is to ensure balance between scaffold degradation and neo-tissue formation. We evaluated the rate of degradation and neo-tissue formation of three electrospun supramolecular bisurea-based biodegradable scaffolds that differ in their soft-block backbone compositions only. Scaffolds were implanted as interposition grafts in the abdominal aorta in rats, and evaluated at different time points (t = 1, 6, 12, 24, and 40 weeks) on function, tissue formation, strength, and scaffold degradation. The fully carbonate-based biomaterial showed minor degradation after 40 weeks in vivo, whereas the other two ester-containing biomaterials showed (near) complete degradation within 6-12 weeks. Local dilatation was only observed in these faster degrading scaffolds. All materials showed to some extent mineralization, at early as well as late time points. Histological evaluation showed equal and non-native-like neo-tissue formation after total degradation. The fully carbonate-based scaffolds lagged in neo-tissue formation, presumably as its degradation was (far from) complete at 40 weeks. A significant difference in vessel wall contrast enhancement was observed by magnetic resonance imaging between grafts with total compared with minimal-degraded scaffolds.
Document Type: Article
Language: English
ISSN: 1937-335X
1937-3341
DOI: 10.1089/ten.tea.2023.0019
Access URL: https://pubmed.ncbi.nlm.nih.gov/38420632
https://research.tue.nl/en/publications/c358572f-d54c-4369-9fed-8fc523dbc9cf
https://doi.org/10.1089/ten.TEA.2023.0019
Rights: Mary Ann Liebert TDM
taverne
Accession Number: edsair.doi.dedup.....8b0d61ec3819e659842a9d16e688cca4
Database: OpenAIRE
Description
Abstract:An essential aspect of cardiovascular in situ tissue engineering (TE) is to ensure balance between scaffold degradation and neo-tissue formation. We evaluated the rate of degradation and neo-tissue formation of three electrospun supramolecular bisurea-based biodegradable scaffolds that differ in their soft-block backbone compositions only. Scaffolds were implanted as interposition grafts in the abdominal aorta in rats, and evaluated at different time points (t = 1, 6, 12, 24, and 40 weeks) on function, tissue formation, strength, and scaffold degradation. The fully carbonate-based biomaterial showed minor degradation after 40 weeks in vivo, whereas the other two ester-containing biomaterials showed (near) complete degradation within 6-12 weeks. Local dilatation was only observed in these faster degrading scaffolds. All materials showed to some extent mineralization, at early as well as late time points. Histological evaluation showed equal and non-native-like neo-tissue formation after total degradation. The fully carbonate-based scaffolds lagged in neo-tissue formation, presumably as its degradation was (far from) complete at 40 weeks. A significant difference in vessel wall contrast enhancement was observed by magnetic resonance imaging between grafts with total compared with minimal-degraded scaffolds.
ISSN:1937335X
19373341
DOI:10.1089/ten.tea.2023.0019