Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles
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| Titel: | Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles |
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| Autoren: | Borges, Olga, Cordeiro-da-Silva, Anabela, Tavares, Joana, Santarém, Nuno, de Sousa, Adriano, Borchard, Gerrit, Junginger, Hans E. |
| Quelle: | European Journal of Pharmaceutics and Biopharmaceutics, Vol. 69, No 2 (2008) pp. 405-16 |
| Verlagsinformationen: | Elsevier BV, 2008. |
| Publikationsjahr: | 2008 |
| Schlagwörter: | 0301 basic medicine, Polymers, Intranasal vaccination, Alginates, Oligonucleotides, Enzyme-Linked Immunosorbent Assay, Mice Inbred BALB C, Hepatitis B Vaccines/*administration & dosage/*immunology, Interferon-gamma, Mice, 03 medical and health sciences, Animals, Hepatitis B Vaccines, Hepatitis B Antibodies, Alginate coated chitosan nanoparticles, Immunity, Mucosal, Administration, Intranasal, Cell Proliferation, Spleen/cytology/immunology/metabolism, ddc:615, Vaccines, Chitosan, Drug Carriers, Mice, Inbred BALB C, 0303 health sciences, Hepatitis B Surface Antigens, Immunity Mucosal/immunology, Hepatitis B Surface Antigens/*administration & dosage/*immunology, Oligonucleotides/*administration & dosage, 3. Good health, CpG oligodeoxynucleotide, Intranasal, Hepatitis B surface antigen, Administration, Hepatitis B Antibodies/*biosynthesis, Antibody Formation, Nanoparticles, CpG Islands, Female, Indicators and Reagents |
| Beschreibung: | Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-gamma production in supernatants of the spleen cells. The mice were primed with 10 microg of the vaccine associated or not with nanoparticles and associated or not with 10 microg CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immunopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-gamma production when compared to naïve mice. |
| Publikationsart: | Article |
| Dateibeschreibung: | aplication/PDF; application/pdf |
| Sprache: | English |
| ISSN: | 0939-6411 |
| DOI: | 10.1016/j.ejpb.2008.01.019 |
| Zugangs-URL: | https://estudogeral.sib.uc.pt/bitstream/10316/5833/1/filecd94f91b202846b3a5e7ce8d7a7b66b9.pdf https://pubmed.ncbi.nlm.nih.gov/18364251 https://pubmed.ncbi.nlm.nih.gov/18364251/ https://estudogeral.sib.uc.pt/handle/10316/5833 https://www.sciencedirect.com/science/article/pii/S0939641108000234 https://archive-ouverte.unige.ch/unige:819 https://europepmc.org/article/MED/18364251 https://estudogeral.sib.uc.pt/bitstream/10316/5833/1/filecd94f91b202846b3a5e7ce8d7a7b66b9.pdf https://hdl.handle.net/10316/5833 https://archive-ouverte.unige.ch/unige:819 https://doi.org/10.1016/j.ejpb.2008.01.019 https://archive-ouverte.unige.ch/unige:819 |
| Rights: | Elsevier TDM |
| Dokumentencode: | edsair.doi.dedup.....899e40b8fded87fb4f7981b3781c9a56 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-gamma production in supernatants of the spleen cells. The mice were primed with 10 microg of the vaccine associated or not with nanoparticles and associated or not with 10 microg CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immunopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-gamma production when compared to naïve mice. |
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| ISSN: | 09396411 |
| DOI: | 10.1016/j.ejpb.2008.01.019 |