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Essential phospholipids impact cytokine secretion and alter lipid-metabolizing enzymes in human hepatocyte cell lines

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Title: Essential phospholipids impact cytokine secretion and alter lipid-metabolizing enzymes in human hepatocyte cell lines
Authors: Wupperfeld, Dominik, Fricker, Gert, Bois De Fer, Béatrice, Popovic, Branko
Source: Pharmacol Rep
Publisher Information: Springer Science and Business Media LLC, 2024.
Publication Year: 2024
Subject Terms: Lipopolysaccharides, 0301 basic medicine, 0303 health sciences, Interleukin-6, Interleukin-8, Hep G2 Cells, Lipid Metabolism, Article, Cell Line, 03 medical and health sciences, Hepatocytes, Humans, Cytokines, Interleukin-8/metabolism [MeSH], Essential phospholipids, HepaRG, Humans [MeSH], Cell Line [MeSH], Lipopolysaccharides/pharmacology [MeSH], Cytokines/metabolism [MeSH], Hep G2 Cells [MeSH], Hepatocytes/metabolism [MeSH], Lipid Metabolism/drug effects [MeSH], Non-alcoholic fatty liver disease, HepG2, Steatotic HepaRG, Phospholipids/metabolism [MeSH], Interleukin-6/metabolism [MeSH], Hepatocytes/drug effects [MeSH], Phospholipids
Description: Background Essential phospholipids (EPL) are hepatoprotective. Methods The effects on interleukin (IL)-6 and -8 secretion and on certain lipid-metabolizing enzymes of non-cytotoxic concentrations of EPL (0.1 and 0.25 mg/ml), polyenylphosphatidylcholine (PPC), and phosphatidylinositol (PtdIns) (both at 0.1 and 1 mg/ml), compared with untreated controls, were assessed in human hepatocyte cell lines (HepG2, HepaRG, and steatotic HepaRG). Results Lipopolysaccharide (LPS)-induced IL-6 secretion was significantly decreased in HepaRG cells by most phospholipids, and significantly increased in steatotic HepaRG cells with at least one concentration of EPL and PtdIns. LPS-induced IL-8 secretion was significantly increased in HepaRG and steatotic HepaRG cells with all phospholipids. All phospholipids significantly decreased amounts of fatty acid synthase in steatotic HepaRG cells and the amounts of acyl-CoA oxidase in HepaRG cells. Amounts of lecithin cholesterol acyltransferase were significantly decreased in HepG2 and HepaRG cells by most phospholipids, and significantly increased with 0.1 mg/ml PPC (HepaRG cells) and 1 mg/ml PtdIns (steatotic HepaRG cells). Glucose-6-phosphate dehydrogenase activity was unaffected by any phospholipid in any cell line. Conclusions EPL, PPC, and PtdIns impacted the secretion of pro-inflammatory cytokines and affected amounts of several key lipid-metabolizing enzymes in human hepatocyte cell lines. Such changes may help liver function improvement, and provide further insights into the EPL’s mechanism of action.
Document Type: Article
Other literature type
Language: English
ISSN: 2299-5684
1734-1140
DOI: 10.1007/s43440-024-00595-4
Access URL: https://pubmed.ncbi.nlm.nih.gov/38664334
https://repository.publisso.de/resource/frl:6519053
Rights: CC BY
Accession Number: edsair.doi.dedup.....8923d1f98343e2848e26f61f1d36b912
Database: OpenAIRE
Description
Abstract:Background Essential phospholipids (EPL) are hepatoprotective. Methods The effects on interleukin (IL)-6 and -8 secretion and on certain lipid-metabolizing enzymes of non-cytotoxic concentrations of EPL (0.1 and 0.25 mg/ml), polyenylphosphatidylcholine (PPC), and phosphatidylinositol (PtdIns) (both at 0.1 and 1 mg/ml), compared with untreated controls, were assessed in human hepatocyte cell lines (HepG2, HepaRG, and steatotic HepaRG). Results Lipopolysaccharide (LPS)-induced IL-6 secretion was significantly decreased in HepaRG cells by most phospholipids, and significantly increased in steatotic HepaRG cells with at least one concentration of EPL and PtdIns. LPS-induced IL-8 secretion was significantly increased in HepaRG and steatotic HepaRG cells with all phospholipids. All phospholipids significantly decreased amounts of fatty acid synthase in steatotic HepaRG cells and the amounts of acyl-CoA oxidase in HepaRG cells. Amounts of lecithin cholesterol acyltransferase were significantly decreased in HepG2 and HepaRG cells by most phospholipids, and significantly increased with 0.1 mg/ml PPC (HepaRG cells) and 1 mg/ml PtdIns (steatotic HepaRG cells). Glucose-6-phosphate dehydrogenase activity was unaffected by any phospholipid in any cell line. Conclusions EPL, PPC, and PtdIns impacted the secretion of pro-inflammatory cytokines and affected amounts of several key lipid-metabolizing enzymes in human hepatocyte cell lines. Such changes may help liver function improvement, and provide further insights into the EPL’s mechanism of action.
ISSN:22995684
17341140
DOI:10.1007/s43440-024-00595-4