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Role of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male rats: Elevated nNOS expression from distal colon of NMS rats

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Název: Role of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male rats: Elevated nNOS expression from distal colon of NMS rats
Autoři: Lixing Lao, Yung-Wui Tjong, Siu-Po Ip, Justin C.Y. Wu, Joseph J.Y. Sung, Brian Berman, Harry Hs Fong, Chun-Tao Che
Zdroj: Neurogastroenterology & Motility. 23:666-e278
Informace o vydavateli: Wiley, 2011.
Rok vydání: 2011
Témata: Male, 0301 basic medicine, Irritable Bowel Syndrome - Physiopathology, Ng-Nitroarginine Methyl Ester - Pharmacology, Indazoles, Nitric Oxide Synthase Type III, Colon, Colon - Physiopathology, Nitric Oxide Synthase Type II, Nitric Oxide Synthase Type I, Stress, Colonic Diseases - Physiopathology, Irritable Bowel Syndrome, Rats, Sprague-Dawley, Colonic Diseases, 03 medical and health sciences, Psychological - Physiopathology, Animals, Maternal Behavior, Stress, Psychological - Physiopathology, 0303 health sciences, Animal, Newborn - Physiology, Electromyography, Maternal Behavior - Physiology, Hyperalgesia - Physiopathology, Rats, 3. Good health, Nitric Oxide Synthase Type Iii - Antagonists & Inhibitors - Drug Effects - Physiology, Disease Models, Animal, Oxidative Stress, NG-Nitroarginine Methyl Ester, Animals, Newborn, Hyperalgesia, Disease Models, Gastrointestinal Motility - Physiology, Oxidative Stress - Drug Effects, Indazoles - Pharmacology, Sprague-Dawley, Nitric Oxide Synthase Type Ii - Antagonists & Inhibitors - Drug Effects - Physiology, Gastrointestinal Motility, Nitric Oxide Synthase Type I - Antagonists & Inhibitors - Drug Effects - Physiology, Animals, Newborn - Physiology, Stress, Psychological
Popis: Nitric oxide (NO) is implicated in the pathogenesis of irritable bowel syndrome (IBS) but the underlying mechanism is unclear. Thus, the aim of the present study is to examine the role of NO synthase (NOS) expression in the distal colon of neonatal maternal separation (NMS) model rats employed in IBS studies.Male neonates of Sprague-Dawley rats were randomly assigned into NMS and normal control (N) groups. Rats of NMS group were subjected to 3 h daily maternal separation on postnatal day 2-21. Rats were administrated non-selective NOS inhibitor l-NAME (100 mg kg(-1) ), selective neuronal NOS (nNOS) inhibitor 7-NINA (10mgkg(-1) ), selective inducible NOS (iNOS) inhibitor, endothelial NOS (eNOS) inhibitor (10mgkg(-1) ) or Vehicle (Veh; distilled water) intraperitoneally 1h prior to the experiment for the test and control groups, respectively.The amount of NO was significantly higher in the NMS Veh rats compared with unseparated N rats. Western-blotting and real-time quantitative PCR studies showed that protein and mRNA expression of nNOS were higher in the NMS group than that in the N rats; whereas no significant change in iNOS and eNOS was found in either groups. Neonatal maternal separation Veh rats showed low pain threshold and increased electromyogram (EMG) activity in response to colonic distension stimuli. l-NAME and 7-Nitroindazole monosodium salt (7-NINA) increased pain threshold pressure and attenuated EMG activity in the NMS rats. In addition, l-NAME and 7-NINA substantially reduced oxidative marker malondialdehyde level in NMS rats.Neonatal maternal separation increased the NO generation by nNOS upregulation that interact with reactive oxygen species contributing to the visceral hypersensitivity in IBS.
Druh dokumentu: Article
Jazyk: English
ISSN: 1350-1925
DOI: 10.1111/j.1365-2982.2011.01697.x
Přístupová URL adresa: https://europepmc.org/articles/pmc3117987?pdf=render
https://pubmed.ncbi.nlm.nih.gov/21410601
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3117987
https://core.ac.uk/display/38030277
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2982.2011.01697.x/full
https://europepmc.org/articles/PMC3117987
https://hub.hku.hk/handle/10722/188631
https://pubmed.ncbi.nlm.nih.gov/21410601/
http://hdl.handle.net/10722/188631
Rights: Wiley TDM
Přístupové číslo: edsair.doi.dedup.....88fa146b19ab000f1f168991b50e537c
Databáze: OpenAIRE
Popis
Abstrakt:Nitric oxide (NO) is implicated in the pathogenesis of irritable bowel syndrome (IBS) but the underlying mechanism is unclear. Thus, the aim of the present study is to examine the role of NO synthase (NOS) expression in the distal colon of neonatal maternal separation (NMS) model rats employed in IBS studies.Male neonates of Sprague-Dawley rats were randomly assigned into NMS and normal control (N) groups. Rats of NMS group were subjected to 3 h daily maternal separation on postnatal day 2-21. Rats were administrated non-selective NOS inhibitor l-NAME (100 mg kg(-1) ), selective neuronal NOS (nNOS) inhibitor 7-NINA (10mgkg(-1) ), selective inducible NOS (iNOS) inhibitor, endothelial NOS (eNOS) inhibitor (10mgkg(-1) ) or Vehicle (Veh; distilled water) intraperitoneally 1h prior to the experiment for the test and control groups, respectively.The amount of NO was significantly higher in the NMS Veh rats compared with unseparated N rats. Western-blotting and real-time quantitative PCR studies showed that protein and mRNA expression of nNOS were higher in the NMS group than that in the N rats; whereas no significant change in iNOS and eNOS was found in either groups. Neonatal maternal separation Veh rats showed low pain threshold and increased electromyogram (EMG) activity in response to colonic distension stimuli. l-NAME and 7-Nitroindazole monosodium salt (7-NINA) increased pain threshold pressure and attenuated EMG activity in the NMS rats. In addition, l-NAME and 7-NINA substantially reduced oxidative marker malondialdehyde level in NMS rats.Neonatal maternal separation increased the NO generation by nNOS upregulation that interact with reactive oxygen species contributing to the visceral hypersensitivity in IBS.
ISSN:13501925
DOI:10.1111/j.1365-2982.2011.01697.x