Molecular and Physiological Diversity of Nicotinic Acetylcholine Receptors in the Midbrain Dopaminergic Nuclei
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| Titel: | Molecular and Physiological Diversity of Nicotinic Acetylcholine Receptors in the Midbrain Dopaminergic Nuclei |
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| Autoren: | Alban de Kerchove d'Exaerde, Michele Zoli, Jean-Pierre Changeux, Ruby Klink |
| Quelle: | The Journal of neuroscience, 21 (5 |
| Verlagsinformationen: | Society for Neuroscience, 2001. |
| Publikationsjahr: | 2001 |
| Schlagwörter: | 0301 basic medicine, Patch-Clamp Techniques, Nicotinic Antagonists -- administration & dosage, Dopamine, Substantia Nigra -- drug effects, Nicotinic Antagonists, Neurons -- drug effects, Receptors, Nicotinic, Rats, Sprague-Dawley, Mice, Tyrosine 3-Monooxygenase -- genetics, Receptors, Receptors, Nicotinic -- deficiency, Nicotinic Agonists, Glutamate Decarboxylase -- metabolism, Neurons, 0303 health sciences, Glutamate Decarboxylase, Reverse Transcriptase Polymerase Chain Reaction, Sciences bio-médicales et agricoles, Nicotinic Agonists -- administration & dosage, Mutant Strains, Substantia Nigra -- metabolism, Substantia Nigra, Dopamine -- metabolism, Nicotinic -- genetics, Nicotinic -- deficiency, addiction, dopamine, GABA, nAChR, Parkinson's disease, substantia nigra, ventral tegmental area, Microinjections, Tyrosine 3-Monooxygenase, Glutamate Decarboxylase -- genetics, Receptors, Nicotinic -- genetics, In Vitro Techniques, 03 medical and health sciences, Animals, Neurons -- metabolism, RNA, Messenger, Neurons -- cytology, Ventral Tegmental Area -- metabolism, Nicotinic -- metabolism, Ventral Tegmental Area -- drug effects, Ventral Tegmental Area, Receptors, Nicotinic -- metabolism, RNA, Messenger -- metabolism, Mice, Mutant Strains, Rats, Messenger -- metabolism, Protein Subunits, Neurons -- classification, Tyrosine 3-Monooxygenase -- metabolism, RNA, Sprague-Dawley, gamma-Aminobutyric Acid -- metabolism |
| Beschreibung: | Nicotinic acetylcholine receptors (nAChRs) on dopaminergic (DA) and GABAergic (Gaba) projection neurons of the substantia nigra (SN) and ventral tegmental area (VTA) are characterized by single-cell RT-PCR and patch-clamp recordings in slices of rat and wild-type, beta2-/-, alpha4-/-, and alpha7-/- mice. The eight nAChR subunits expressed in these nuclei, alpha3-7 and beta2-4, contribute to four different types of nAChR-mediated currents. Most DA neurons in the SN and VTA express two nAChR subtypes. One is inhibited by dihydro-beta-erythroidine (2 microm), alpha-conotoxin MII (10 nm), and methyllycaconitine (1 nm) but does not contain the alpha7 subunit; it possesses a putative alpha4alpha6alpha5(beta2)(2) composition. The other subtype is inhibited by dihydro-beta-erythroidine (2 microm) and has a putative alpha4alpha5(beta2)(2) composition. Gaba neurons in the VTA exhibit a third subtype with a putative (alpha4)(2)(beta2)(3) composition, whereas Gaba neurons in the SN have either the putative (alpha4)(2)(beta2)(3) oligomer or the putative alpha4alpha6alpha5(beta2)(2) oligomer. The fourth subtype, a putative (alpha7)(5) homomer, is encountered in less than half of DA and Gaba neurons, in the SN as well as in the VTA. Neurons in the DA nuclei thus exhibit a diversity of nAChRs that might differentially modulate reinforcement and motor behavior. |
| Publikationsart: | Article |
| Dateibeschreibung: | 1 full-text file(s): application/pdf |
| Sprache: | English |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.21-05-01452.2001 |
| Zugangs-URL: | http://www.jneurosci.org/content/jneuro/21/5/1452.full.pdf https://pubmed.ncbi.nlm.nih.gov/11222635 https://iris.unimore.it/handle/11380/304169 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50701/Holdings https://www.jneurosci.org/content/jneuro/21/5/1452.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762941/ https://www.jneurosci.org/content/21/5/1452 http://europepmc.org/abstract/MED/11222635 http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50701 |
| Dokumentencode: | edsair.doi.dedup.....86dedc8892e1d87c3d7a2f1e33b77091 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Nicotinic acetylcholine receptors (nAChRs) on dopaminergic (DA) and GABAergic (Gaba) projection neurons of the substantia nigra (SN) and ventral tegmental area (VTA) are characterized by single-cell RT-PCR and patch-clamp recordings in slices of rat and wild-type, beta2-/-, alpha4-/-, and alpha7-/- mice. The eight nAChR subunits expressed in these nuclei, alpha3-7 and beta2-4, contribute to four different types of nAChR-mediated currents. Most DA neurons in the SN and VTA express two nAChR subtypes. One is inhibited by dihydro-beta-erythroidine (2 microm), alpha-conotoxin MII (10 nm), and methyllycaconitine (1 nm) but does not contain the alpha7 subunit; it possesses a putative alpha4alpha6alpha5(beta2)(2) composition. The other subtype is inhibited by dihydro-beta-erythroidine (2 microm) and has a putative alpha4alpha5(beta2)(2) composition. Gaba neurons in the VTA exhibit a third subtype with a putative (alpha4)(2)(beta2)(3) composition, whereas Gaba neurons in the SN have either the putative (alpha4)(2)(beta2)(3) oligomer or the putative alpha4alpha6alpha5(beta2)(2) oligomer. The fourth subtype, a putative (alpha7)(5) homomer, is encountered in less than half of DA and Gaba neurons, in the SN as well as in the VTA. Neurons in the DA nuclei thus exhibit a diversity of nAChRs that might differentially modulate reinforcement and motor behavior. |
|---|---|
| ISSN: | 15292401 02706474 |
| DOI: | 10.1523/jneurosci.21-05-01452.2001 |