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Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

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Titel: Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity
Autoren: Katarzyna Niemirowicz, Jan Ryszard Dąbrowski, Mateusz Cieśluk, Agnieszka Z. Wilczewska, Ewelina Piktel, Piotr Deptuła, Grażyna Tokajuk, Robert Bucki
Quelle: Int J Nanomedicine
International Journal of Nanomedicine, Vol Volume 12, Pp 7833-7846 (2017)
Verlagsinformationen: Informa UK Limited, 2017.
Publikationsjahr: 2017
Schlagwörter: 0301 basic medicine, magnetic nanoparticles, Medicine (General), antimicrobial properties, Plankton - microbiology, Microbial Sensitivity Tests, Microbial sensitivity tests, Gram-Positive Bacteria, Gram-negative bacteria - drug effects, Cell Line, 03 medical and health sciences, R5-920, Drug Delivery Systems, Anti-Infective Agents, International Journal of Nanomedicine, Gram-Negative Bacteria, Interleukin-8 - metabolism, Gram-positive bacteria - drug effects, Humans, anti-biofilm, Magnetite Nanoparticles, Chlorhexidine - administration & dosage, Original Research, Osteoblasts - drug effects, 0303 health sciences, Osteoblasts, Chlorhexidine - pharmacology, chlorhexidine, Chlorhexidine, Interleukin-8, Plankton, 16. Peace & justice, Drug delivery systems - methods, Osteoblasts - metabolism, 3. Good health, Oxidative stress - drug effects, Biofilms - drug effects, Oxidative Stress, Anti-infective agents - administration & dosage, Biofilms, Magnetite nanoparticles - administration & dosage, Anti-infective agents - pharmacology, Magnetite nanoparticles - chemistry, Cell line
Beschreibung: Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX). In the presence of human saliva, MNPs@CHX displayed significantly greater bactericidal and fungicidal activity against planktonic and biofilm-forming microorganisms than free CHX. In addition, CHX attached to MNPs has an increased ability to restrict the growth of mixed-species biofilms compared to free CHX. The observed depolarization of mitochondria in fungal cells treated with MNP@CHX suggests that induction of oxidative stress and oxidation of fungal structures may be a part of the mechanism responsible for pathogen killing. Nanoparticles functionalized by CHX did not affect host cell proliferation or their ability to release the proinflammatory cytokine, IL-8. The use of MNPs as a carrier of CHX has great potential for the development of antiseptic nanosystems.
Publikationsart: Article
Other literature type
Dateibeschreibung: text/html; application/pdf
Sprache: English
ISSN: 1178-2013
DOI: 10.2147/ijn.s140661
Zugangs-URL: https://www.dovepress.com/getfile.php?fileID=39028
https://pubmed.ncbi.nlm.nih.gov/29123396
https://doaj.org/article/70139e271e2f4264b4c77848d4274809
https://www.dovepress.com/use-of-magnetic-nanoparticles-as-a-drug-delivery-system-to-improve-chl-peer-reviewed-article-IJN
https://europepmc.org/articles/PMC5661836
https://www.ncbi.nlm.nih.gov/pubmed/29123396
https://www.dovepress.com/getfile.php?fileID=39028
https://pubmed.ncbi.nlm.nih.gov/29123396/
https://www.dovepress.com/use-of-magnetic-nanoparticles-as-a-drug-delivery-system-to-improve-chl-peer-reviewed-fulltext-article-IJN
Rights: CC BY NC
Dokumentencode: edsair.doi.dedup.....85cbc35b50c4d90dcfc36c16e0d28752
Datenbank: OpenAIRE
Beschreibung
Abstract:Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX). In the presence of human saliva, MNPs@CHX displayed significantly greater bactericidal and fungicidal activity against planktonic and biofilm-forming microorganisms than free CHX. In addition, CHX attached to MNPs has an increased ability to restrict the growth of mixed-species biofilms compared to free CHX. The observed depolarization of mitochondria in fungal cells treated with MNP@CHX suggests that induction of oxidative stress and oxidation of fungal structures may be a part of the mechanism responsible for pathogen killing. Nanoparticles functionalized by CHX did not affect host cell proliferation or their ability to release the proinflammatory cytokine, IL-8. The use of MNPs as a carrier of CHX has great potential for the development of antiseptic nanosystems.
ISSN:11782013
DOI:10.2147/ijn.s140661