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Physico-chemical–mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates

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Title: Physico-chemical–mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates
Authors: Guy Daculsi, Jérôme Guicheux, Marion Julien, Pierre Weiss, Jean-Michel Bouler, Racquel Z. LeGeros, Séverine Delplace, Ibrahim Khairoun, Paul Pilet
Contributors: Weiss, Pierre, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR Odontologie (UFR Odonto), Université de Nantes (UN)-Université de Nantes (UN), Department of Biomaterials and Biomimetics, New York University College of Dentistry, NYU System (NYU)-NYU System (NYU)
Source: Biomaterials. 28:956-965
Publisher Information: Elsevier BV, 2007.
Publication Year: 2007
Subject Terms: Calcium Phosphates, MESH: 3T3 Cells, MESH: Materials Testing, Cell Survival, Surface Properties, Molecular Conformation, MESH: Bone Cements, 02 engineering and technology, Mice, 03 medical and health sciences, MESH: Porosity, 0302 clinical medicine, Hardness, Materials Testing, Animals, MESH: Animals, MESH: Particle Size, Dopant, Particle Size, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Mice, MESH: Cell Size, Cell Size, MESH: Surface Properties, MESH: Osteoblasts, MESH: Molecular Conformation, Osteoblasts, Bone Cements, 3T3 Cells, MESH: Calcium Phosphates, Calcium phosphate cements, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Cell Survival, Biocompatibility, 0210 nano-technology, MESH: Hardness, Porosity
Description: Calcium phosphate cements (CPCs) are successfully used as bone substitutes in dentistry and orthopaedic applications. This study investigated the physico-chemical-mechanical properties of and in vitro biological properties (cell response) of CPCs prepared with amorphous calcium carbonate phosphate (ACCP) doped with magnesium (ACCP-Mg), zinc (ACCp-Zn) or fluoride (ACCP-F) ions. The experimental CPC consisted of alpha-TCP, doped ACCP, and MPCM powders as matrix and biphasic calcium phosphate (BCP) granules. X-ray diffraction analysis showed that the matrix converted to apatite with poor crystallinity (reflecting small crystal size) after setting for 24 h, while BCP remained apparently unchanged. Cements with ACCP-F (F-CPC) had shorter setting times and greater compressive strength compared to cements with ACCP-Mg (Mg-CPC) or ACCP-Zn (Zn-CPC). Scanning electron microscopy (SEM) showed that crystals set on Mg-CPC and Zn-CPC were smaller compared to those on F-CPC. The total porosity of Mg-CPC was greater compared to Zn-CPC or F-CPC. Osteoblast-like cells, MC3T3-E1, remained viable and maintained their ability to express alkaline phosphatase in contact with the CPCs with doped ACCPs.
Document Type: Article
File Description: application/pdf
Language: English
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2006.10.018
Access URL: https://www.hal.inserm.fr/inserm-00170245/file/Biomaterials_IK_cpc.pdf
https://pubmed.ncbi.nlm.nih.gov/17123598
https://www.hal.inserm.fr/inserm-00170245
https://www.ncbi.nlm.nih.gov/pubmed/17123598
https://www.hal.inserm.fr/inserm-00170245/document
https://www.sciencedirect.com/science/article/pii/S0142961206009203
http://www.sciencedirect.com/science/article/pii/S0142961206009203
https://inserm.hal.science/inserm-00170245v1
https://inserm.hal.science/inserm-00170245v1/document
https://doi.org/10.1016/j.biomaterials.2006.10.018
Rights: Elsevier TDM
Accession Number: edsair.doi.dedup.....82fdb148a4011b8bfe805593bdfbf4e6
Database: OpenAIRE
Description
Abstract:Calcium phosphate cements (CPCs) are successfully used as bone substitutes in dentistry and orthopaedic applications. This study investigated the physico-chemical-mechanical properties of and in vitro biological properties (cell response) of CPCs prepared with amorphous calcium carbonate phosphate (ACCP) doped with magnesium (ACCP-Mg), zinc (ACCp-Zn) or fluoride (ACCP-F) ions. The experimental CPC consisted of alpha-TCP, doped ACCP, and MPCM powders as matrix and biphasic calcium phosphate (BCP) granules. X-ray diffraction analysis showed that the matrix converted to apatite with poor crystallinity (reflecting small crystal size) after setting for 24 h, while BCP remained apparently unchanged. Cements with ACCP-F (F-CPC) had shorter setting times and greater compressive strength compared to cements with ACCP-Mg (Mg-CPC) or ACCP-Zn (Zn-CPC). Scanning electron microscopy (SEM) showed that crystals set on Mg-CPC and Zn-CPC were smaller compared to those on F-CPC. The total porosity of Mg-CPC was greater compared to Zn-CPC or F-CPC. Osteoblast-like cells, MC3T3-E1, remained viable and maintained their ability to express alkaline phosphatase in contact with the CPCs with doped ACCPs.
ISSN:01429612
DOI:10.1016/j.biomaterials.2006.10.018