Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles
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| Title: | Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles |
|---|---|
| Authors: | Hyunryung Kim, WonHyoung Ryu, Yoon Ha, Lihua Che |
| Contributors: | Hyunryung Kim, Lihua Che, Yoon Ha, WonHyoung Ryu, Che, Lihua, Ha, Yoon, Kim, Hyun Ryung |
| Source: | Materials Science and Engineering: C. 40:324-335 |
| Publisher Information: | Elsevier BV, 2014. |
| Publication Year: | 2014 |
| Subject Terms: | Cell Survival, Nanofibers, Silk, Silk fibroin, Biocompatible Materials, 02 engineering and technology, 01 natural sciences, Hydroxyapatite, Cell Line, Biocompatible Materials/toxicity, Silk/chemistry, Tensile Strength, Humans, Composite scaffold, Electrospinning, Viscosity, Nanoparticles/chemistry, Nanofibers/chemistry, Durapatite/chemistry, Nanoparticles/toxicity, 0104 chemical sciences, Durapatite, Biocompatible Materials/chemistry, Nanoparticles, Fibroins/chemistry, Fibroins, 0210 nano-technology, Mechanical strength, Cell Survival/drug effects |
| Description: | Electrospun silk fibroin (SF) scaffolds provide large surface area, high porosity, and interconnection for cell adhesion and proliferation and they may replace collagen for many tissue engineering applications. Despite such advantages, electrospun SF scaffolds are still limited as bone tissue replacement due to their low mechanical strengths. While enhancement of mechanical strengths by incorporating inorganic ceramics into polymers has been demonstrated, electrospinning of a mixture of SF and inorganic ceramics such as hydroxyapatite is challenging and less studied due to the aggregation of ceramic particles within SF. In this study, we aimed to enhance the mechanical properties of electrospun SF scaffolds by uniformly dispersing hydroxyapatite (HAp) nanoparticles within SF nanofibers. HAp nanoaprticles were modified by γ-glycidoxypropyltrimethoxysilane (GPTMS) for uniform dispersion and enhanced interfacial bonding between HAp and SF fibers. Optimal conditions for electrospinning of SF and GPTMS-modified HAp nanoparticles were identified to achieve beadless nanofibers without any aggregation of HAp nanoparticles. The MTT and SEM analysis of the osteoblasts-cultured scaffolds confirmed the biocompatibility of the composite scaffolds. The mechanical properties of the composite scaffolds were analyzed by tensile tests for the scaffolds with varying contents of HAp within SF fibers. The mechanical testing showed the peak strengths at the HAp content of 20 wt.%. The increase of HAp content up to 20 wt.% increased the mechanical properties of the composite scaffolds, while further increase above 20 wt.% disrupted the polymer chain networks within SF nanofibers and weakened the mechanical strengths. |
| Document Type: | Article |
| File Description: | 324~335 |
| Language: | English |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2014.04.012 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/24857500 https://yonsei.pure.elsevier.com/en/publications/mechanically-reinforced-electrospun-composite-silk-fibroin-nanofi http://www.sciencedirect.com/science/article/pii/S0928493114002045 https://europepmc.org/article/MED/24857500 https://www.sciencedirect.com/science/article/pii/S0928493114002045 https://www.ncbi.nlm.nih.gov/pubmed/24857500 |
| Rights: | CC BY NC ND |
| Accession Number: | edsair.doi.dedup.....80d760e324c4a8fd2e908a877b0a2707 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Electrospun silk fibroin (SF) scaffolds provide large surface area, high porosity, and interconnection for cell adhesion and proliferation and they may replace collagen for many tissue engineering applications. Despite such advantages, electrospun SF scaffolds are still limited as bone tissue replacement due to their low mechanical strengths. While enhancement of mechanical strengths by incorporating inorganic ceramics into polymers has been demonstrated, electrospinning of a mixture of SF and inorganic ceramics such as hydroxyapatite is challenging and less studied due to the aggregation of ceramic particles within SF. In this study, we aimed to enhance the mechanical properties of electrospun SF scaffolds by uniformly dispersing hydroxyapatite (HAp) nanoparticles within SF nanofibers. HAp nanoaprticles were modified by γ-glycidoxypropyltrimethoxysilane (GPTMS) for uniform dispersion and enhanced interfacial bonding between HAp and SF fibers. Optimal conditions for electrospinning of SF and GPTMS-modified HAp nanoparticles were identified to achieve beadless nanofibers without any aggregation of HAp nanoparticles. The MTT and SEM analysis of the osteoblasts-cultured scaffolds confirmed the biocompatibility of the composite scaffolds. The mechanical properties of the composite scaffolds were analyzed by tensile tests for the scaffolds with varying contents of HAp within SF fibers. The mechanical testing showed the peak strengths at the HAp content of 20 wt.%. The increase of HAp content up to 20 wt.% increased the mechanical properties of the composite scaffolds, while further increase above 20 wt.% disrupted the polymer chain networks within SF nanofibers and weakened the mechanical strengths. |
|---|---|
| ISSN: | 09284931 |
| DOI: | 10.1016/j.msec.2014.04.012 |