The apical ECM preserves embryonic integrity and distributes mechanical stress during morphogenesis
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| Názov: | The apical ECM preserves embryonic integrity and distributes mechanical stress during morphogenesis |
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| Autori: | Shashi Kumar Suman, Thanh Thi Kim Vuong-Brender, Michel Labouesse |
| Prispievatelia: | Vuong, Thanh |
| Zdroj: | Development Development (Cambridge) |
| Informácie o vydavateľovi: | The Company of Biologists, 2017. |
| Rok vydania: | 2017 |
| Predmety: | 0301 basic medicine, Leucine-Rich Repeat Proteins, Models, Biological, Zona Pellucida Glycoproteins, 03 medical and health sciences, Laser nano-ablation, [SDV.BDD] Life Sciences [q-bio]/Development Biology, Morphogenesis, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Genes, Helminth, Body Patterning, Muscle anchoring, Extracellular Matrix Proteins, 0303 health sciences, Embryonic elongation, Proteins, Actomyosin, Apical extracellular matrix, Zona Pellucida Protein, Biomechanical Phenomena, Extracellular Matrix, 3. Good health, 13. Climate action, C. elegans, RNA Interference, Stress, Mechanical, Research Article |
| Popis: | Epithelia are bound by both basal and apical extracellular matrices (ECM). While the composition and function of the former have been intensively investigated, less is known about the latter. The embryonic sheath, the ECM apical to the C. elegans embryonic epidermis, has been suggested to promote its elongation. In an RNAi screen for the components of the sheath, we identified the Zona Pellucida domain proteins NOAH-1 and NOAH-2. We found that these proteins act in the same pathway, and in parallel to three other putative sheath proteins, SYM-1, LET-4 and FBN-1/Fibrillin, to ensure embryonic integrity and promote elongation. Laser nano-ablation experiments to map the stress field show that NOAH-1 and NOAH-2, together with PAK-1/p21-activated kinase, maintain and relay the actomyosin-dependent stress generated within the lateral epidermis before muscles become active. Subsequently, loss of function experiments show that apical ECM proteins are essential for muscle anchoring and for relaying the mechanical input from muscle contractions, which are essential for elongation. Hence, the apical ECM contributes to morphogenesis by maintaining embryonic integrity and relaying mechanical stress. |
| Druh dokumentu: | Article Conference object Other literature type |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.150383 |
| Prístupová URL adresa: | https://dev.biologists.org/content/develop/144/23/4336.full.pdf https://pubmed.ncbi.nlm.nih.gov/28526752 https://pubmed.ncbi.nlm.nih.gov/28526752/ https://www.ncbi.nlm.nih.gov/pubmed/28526752 https://www.hal.inserm.fr/inserm-01525862/document https://europepmc.org/abstract/MED/28526752 https://journals.biologists.com/dev/article/144/23/4336/19227/The-apical-ECM-preserves-embryonic-integrity-and https://dev.biologists.org/content/develop/144/23/4336.full.pdf https://inserm.hal.science/inserm-01525862v1 https://doi.org/10.1242/dev.150383 https://inserm.hal.science/inserm-01525862v1/document |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| Prístupové číslo: | edsair.doi.dedup.....7e77e4c9cc8d42b31166a29d758b3539 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Epithelia are bound by both basal and apical extracellular matrices (ECM). While the composition and function of the former have been intensively investigated, less is known about the latter. The embryonic sheath, the ECM apical to the C. elegans embryonic epidermis, has been suggested to promote its elongation. In an RNAi screen for the components of the sheath, we identified the Zona Pellucida domain proteins NOAH-1 and NOAH-2. We found that these proteins act in the same pathway, and in parallel to three other putative sheath proteins, SYM-1, LET-4 and FBN-1/Fibrillin, to ensure embryonic integrity and promote elongation. Laser nano-ablation experiments to map the stress field show that NOAH-1 and NOAH-2, together with PAK-1/p21-activated kinase, maintain and relay the actomyosin-dependent stress generated within the lateral epidermis before muscles become active. Subsequently, loss of function experiments show that apical ECM proteins are essential for muscle anchoring and for relaying the mechanical input from muscle contractions, which are essential for elongation. Hence, the apical ECM contributes to morphogenesis by maintaining embryonic integrity and relaying mechanical stress. |
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| ISSN: | 14779129 09501991 |
| DOI: | 10.1242/dev.150383 |