Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment
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| Názov: | Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment |
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| Autori: | Kamagaju, Léocadie, Morandini, Renato, Bizuru, E., Nyetera, P., Nduwayezu, J.-B., Stévigny, Caroline, Ghanem, Ghanem Elias, Duez, Pierre |
| Zdroj: | Journal of Ethnopharmacology. 146:824-834 |
| Informácie o vydavateľovi: | Elsevier BV, 2013. |
| Rok vydania: | 2013 |
| Predmety: | 0301 basic medicine, Tyrosinase inhibition, Cell Survival, Cell Culture Techniques, Skin Pigmentation, Cosmetics, African Traditional, Skin -- drug effects -- enzymology, Skin Diseases, Skin Pigmentation -- drug effects, Agaricales -- enzymology, Cell Line, Traditional medicine Africa, 03 medical and health sciences, Cell Line, Tumor, Humans, Monophenol Monooxygenase -- antagonists & inhibitors, Medicine, African Traditional, Skin, 0303 health sciences, Tumor, Plants, Medicinal, Monophenol Monooxygenase, Plant Extracts, Rwanda, Medicinal -- chemistry -- growth & development, Skin Diseases -- drug therapy, Plants, 15. Life on land, Dermatologic Agents -- isolation & purification -- pharmacology -- therapeutic use, Cell Survival -- drug effects, 3. Good health, Plant extract, Medicine, Sciences pharmaceutiques, Dermatologic Agents, Agaricales, Plant Extracts -- isolation & purification -- pharmacology -- therapeutic use |
| Popis: | Traditional herbal medicines provide an interesting, largely unexplored source for the development of potential new drugs and skin-care cosmetics. Some herbal extracts are known to be inhibitors of melanin formation, sometimes more potent than the classical inhibitors, hydroquinone/arbutin or kojic acid, and are not associated with melanocytes cytotoxicity or mutagenicity. Such plants are used in traditional medicine in many countries, particularly in Africa, for skin lightening.To evaluate in vitro the ability of Rwandese medicinal plants, traditionally used for the treatment of skin (discoloration and attenuation of discolored spots), to modulate pigmentation and tyrosinase activity.Based on an ethnopharmacological survey, five herbs [Brillantaisia cicatricosa Lindau (Acanthaceae), Chenopodium ugandae (Aellen) Aellen (Chenopodiaceae), Dolichopentas longiflora Oliv. (Rubiaceae), Protea madiensis Oliv. (Proteaceae) and Sesamum angolense Welw. (Pedaliaceae)] were selected. Twenty-seven extracts, obtained by treating the herbs with increasing polarities solvents, were investigated for their effects on cell viability (MTT test) and on pigmentation: inhibition of the enzyme tyrosinase (colorimetry of reaction products, measurement of enzyme activity, TLC-autography; studies on crude cellular extracts obtained from normal melanocytes and on a mushroom tyrosinase) and measurement of melanogenesis by human melanoma cells.None of the tested plant extracts were cytotoxic on tested human melanoma cell lines, except for Dolichopentas longiflora (IC50 of leaves n-hexane extract, 4μg/ml for MM028 and 4.5μg/ml for MM001; IC50 of roots ethyl acetate extract, 0.8μg/ml for MM028 and 3.9μg/ml for MM001). Almost all extracts inhibited melanogenesis in a melanoma whole cells overall pigmentation assay, a model reflecting the entire cycle of melanogenesis. All the Protea madiensis extracts quite strongly inhibited melanogenesis and, surprisingly, one of the Dolichopentas longiflora leaves extracts was found to increase melanogenesis. These results were confirmed by the modulation of pigmentation reactions by crude cellular extracts obtained from normal melanocytes; interestingly, one of the extracts (Dolichopentas longiflora ethyl acetate extract) is even more active (61% at 500μg/ml) than kojic acid ( |
| Druh dokumentu: | Article |
| Popis súboru: | 1 full-text file(s): application/pdf |
| Jazyk: | English |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2013.02.010 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/23439030 https://www.sciencedirect.com/science/article/pii/S0378874113001049 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/136593/Details http://www.ncbi.nlm.nih.gov/pubmed/23439030 https://europepmc.org/article/MED/23439030 https://pubmed.ncbi.nlm.nih.gov/23439030/ |
| Rights: | Elsevier TDM |
| Prístupové číslo: | edsair.doi.dedup.....776d1fc2b6d380bcaf6b62ec8dd10c40 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Traditional herbal medicines provide an interesting, largely unexplored source for the development of potential new drugs and skin-care cosmetics. Some herbal extracts are known to be inhibitors of melanin formation, sometimes more potent than the classical inhibitors, hydroquinone/arbutin or kojic acid, and are not associated with melanocytes cytotoxicity or mutagenicity. Such plants are used in traditional medicine in many countries, particularly in Africa, for skin lightening.To evaluate in vitro the ability of Rwandese medicinal plants, traditionally used for the treatment of skin (discoloration and attenuation of discolored spots), to modulate pigmentation and tyrosinase activity.Based on an ethnopharmacological survey, five herbs [Brillantaisia cicatricosa Lindau (Acanthaceae), Chenopodium ugandae (Aellen) Aellen (Chenopodiaceae), Dolichopentas longiflora Oliv. (Rubiaceae), Protea madiensis Oliv. (Proteaceae) and Sesamum angolense Welw. (Pedaliaceae)] were selected. Twenty-seven extracts, obtained by treating the herbs with increasing polarities solvents, were investigated for their effects on cell viability (MTT test) and on pigmentation: inhibition of the enzyme tyrosinase (colorimetry of reaction products, measurement of enzyme activity, TLC-autography; studies on crude cellular extracts obtained from normal melanocytes and on a mushroom tyrosinase) and measurement of melanogenesis by human melanoma cells.None of the tested plant extracts were cytotoxic on tested human melanoma cell lines, except for Dolichopentas longiflora (IC50 of leaves n-hexane extract, 4μg/ml for MM028 and 4.5μg/ml for MM001; IC50 of roots ethyl acetate extract, 0.8μg/ml for MM028 and 3.9μg/ml for MM001). Almost all extracts inhibited melanogenesis in a melanoma whole cells overall pigmentation assay, a model reflecting the entire cycle of melanogenesis. All the Protea madiensis extracts quite strongly inhibited melanogenesis and, surprisingly, one of the Dolichopentas longiflora leaves extracts was found to increase melanogenesis. These results were confirmed by the modulation of pigmentation reactions by crude cellular extracts obtained from normal melanocytes; interestingly, one of the extracts (Dolichopentas longiflora ethyl acetate extract) is even more active (61% at 500μg/ml) than kojic acid ( |
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| ISSN: | 03788741 |
| DOI: | 10.1016/j.jep.2013.02.010 |