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Polycaprolactone-Coated 3D Printed Tricalcium Phosphate Scaffolds for Bone Tissue Engineering: In Vitro Alendronate Release Behavior and Local Delivery Effect on In Vivo Osteogenesis

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Title: Polycaprolactone-Coated 3D Printed Tricalcium Phosphate Scaffolds for Bone Tissue Engineering: In Vitro Alendronate Release Behavior and Local Delivery Effect on In Vivo Osteogenesis
Authors: Tarafder, Solaiman, Bose, Susmita
Source: ACS Appl Mater Interfaces
Publisher Information: American Chemical Society (ACS), 2014.
Publication Year: 2014
Subject Terms: Alendronate - administration & dosage, 0301 basic medicine, Polyesters, 02 engineering and technology, In Vitro Techniques, Rats, Sprague-Dawley, 03 medical and health sciences, Bone Density Conservation Agents - administration & dosage, Osteogenesis, Animals, Osteogenesis - drug effects, Bone Density Conservation Agents - pharmacology, Alendronate, Bone Density Conservation Agents, Tissue Engineering, Tissue Scaffolds, Polyesters - chemistry, 6. Clean water, Rats, Alendronate - pharmacology, Three-Dimensional, Printing, Three-Dimensional, Printing, Sprague-Dawley, 0210 nano-technology
Description: The aim of this work was to evaluate the effect of in vitro alendronate (AD) release behavior through polycaprolactone (PCL) coating on in vivo bone formation using PCL-coated 3D printed interconnected porous tricalcium phosphate (TCP) scaffolds. Higher AD and Ca(2+) ion release was observed at lower pH (5.0) than that at higher pH (7.4). AD and Ca(2+) release, surface morphology, and phase analysis after release indicated a matrix degradation dominated AD release caused by TCP dissolution. PCL coating showed its effectiveness for controlled and sustained AD release. Six different scaffold compositions, namely, (i) TCP (bare TCP), (ii) TCP + AD (AD-coated TCP), (iii) TCP + PCL (PCL-coated TCP), (iv) TCP + PCL + AD, (v) TCP + AD + PCL, and (vi) TCP + AD + PCL + AD were tested in the distal femoral defect of Sprague-Dawley rats for 6 and 10 weeks. An excellent bone formation inside the micro and macro pores of the scaffolds was observed from histomorphology. Histomorphometric analysis revealed maximum new bone formation in TCP + AD + PCL scaffolds after 6 weeks. No adverse effect of PCL on bioactivity of TCP and in vivo bone formation was observed. All scaffolds with AD showed higher bone formation and reduced TRAP (tartrate resistant acid phosphatase) positive cells activity compared to bare TCP and TCP coated with only PCL. Bare TCP scaffolds showed the highest TRAP positive cells activity followed by TCP + PCL scaffolds, whereas TCP + AD scaffolds showed the lowest TRAP activity. A higher TRAP positive cells activity was observed in TCP + AD + PCL compared to TCP + AD scaffolds after 6 weeks. Our results show that in vivo local AD delivery from PCL-coated 3DP TCP scaffolds could further induce increased early bone formation.
Document Type: Article
Other literature type
Language: English
ISSN: 1944-8252
1944-8244
DOI: 10.1021/am501048n
Access URL: https://pubmed.ncbi.nlm.nih.gov/24826838
https://pubs.acs.org/doi/pdf/10.1021/am501048n
http://pubs.acs.org/doi/full/10.1021/am501048n
https://pubs.acs.org/doi/10.1021/am501048n
http://pubs.acs.org/doi/abs/10.1021/am501048n
http://europepmc.org/articles/PMC4095936
https://pubag.nal.usda.gov/catalog/5276568
Rights: Standard ACS AuthorChoice/Editors’ Choice Usage Agreement
acs-specific: authorchoice/editors choice usage agreement
Accession Number: edsair.doi.dedup.....70209a95ca7b5261b721adf98b191f90
Database: OpenAIRE
Description
Abstract:The aim of this work was to evaluate the effect of in vitro alendronate (AD) release behavior through polycaprolactone (PCL) coating on in vivo bone formation using PCL-coated 3D printed interconnected porous tricalcium phosphate (TCP) scaffolds. Higher AD and Ca(2+) ion release was observed at lower pH (5.0) than that at higher pH (7.4). AD and Ca(2+) release, surface morphology, and phase analysis after release indicated a matrix degradation dominated AD release caused by TCP dissolution. PCL coating showed its effectiveness for controlled and sustained AD release. Six different scaffold compositions, namely, (i) TCP (bare TCP), (ii) TCP + AD (AD-coated TCP), (iii) TCP + PCL (PCL-coated TCP), (iv) TCP + PCL + AD, (v) TCP + AD + PCL, and (vi) TCP + AD + PCL + AD were tested in the distal femoral defect of Sprague-Dawley rats for 6 and 10 weeks. An excellent bone formation inside the micro and macro pores of the scaffolds was observed from histomorphology. Histomorphometric analysis revealed maximum new bone formation in TCP + AD + PCL scaffolds after 6 weeks. No adverse effect of PCL on bioactivity of TCP and in vivo bone formation was observed. All scaffolds with AD showed higher bone formation and reduced TRAP (tartrate resistant acid phosphatase) positive cells activity compared to bare TCP and TCP coated with only PCL. Bare TCP scaffolds showed the highest TRAP positive cells activity followed by TCP + PCL scaffolds, whereas TCP + AD scaffolds showed the lowest TRAP activity. A higher TRAP positive cells activity was observed in TCP + AD + PCL compared to TCP + AD scaffolds after 6 weeks. Our results show that in vivo local AD delivery from PCL-coated 3DP TCP scaffolds could further induce increased early bone formation.
ISSN:19448252
19448244
DOI:10.1021/am501048n