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In-Membrane Nanostructuring of Cationic Amphiphiles Affects Their Antimicrobial Efficacy and Cytotoxicity: A Comparison Study between a De Novo Antimicrobial Lipopeptide and Traditional Biocides

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Název: In-Membrane Nanostructuring of Cationic Amphiphiles Affects Their Antimicrobial Efficacy and Cytotoxicity: A Comparison Study between a De Novo Antimicrobial Lipopeptide and Traditional Biocides
Autoři: Ke Fa, Huayang Liu, Haoning Gong, Lin Zhang, Mingrui Liao, Xuzhi Hu, Daniela Ciumac, Peixun Li, John Webster, Jordan Petkov, Robert K. Thomas, Jian Ren Lu
Zdroj: Langmuir
Fa, K, Liu, H, Gong, H, Zhang, L, Liao, M, Hu, X, Ciumac, D, Li, P, Webster, J, Petkov, J, Thomas, R K & Lu, J R 2022, 'In-Membrane Nanostructuring of Cationic Amphiphiles Affects Their Antimicrobial Efficacy and Cytotoxicity: A Comparison Study between a De Novo Antimicrobial Lipopeptide and Traditional Biocides', Langmuir, vol. 38, no. 21, pp. 6623-6637. https://doi.org/10.1021/acs.langmuir.2c00506
Informace o vydavateli: American Chemical Society (ACS), 2022.
Rok vydání: 2022
Témata: Disinfectants/pharmacology, Lipopeptides/pharmacology, Mammals, 0301 basic medicine, 0303 health sciences, Anti-Bacterial Agents/chemistry, Antimicrobial Cationic Peptides/chemistry, Microbial Sensitivity Tests, Anti-Bacterial Agents, Lipopeptides, 03 medical and health sciences, Anti-Infective Agents, Anti-Infective Agents/toxicity, Animals, Antimicrobial Cationic Peptides, Disinfectants
Popis: Cationic biocides have been widely used as active ingredients in personal care and healthcare products for infection control and wound treatment for a long time, but there are concerns over their cytotoxicity and antimicrobial resistance. Designed lipopeptides are potential candidates for alleviating these issues because of their mildness to mammalian host cells and their high efficacy against pathogenic microbial membranes. In this study, antimicrobial and cytotoxic properties of a de novo designed lipopeptide, CH3(CH2)12CO-Lys-Lys-Gly-Gly-Ile-Ile-NH2 (C14KKGGII), were assessed against that of two traditional cationic biocides CnTAB (n = 12 and 14), with different critical aggregation concentrations (CACs). C14KKGGII was shown to be more potent against both bacteria and fungi but milder to fibroblast host cells than the two biocides. Biophysical measurements mimicking the main features of microbial and host cell membranes were obtained for both lipid monolayer models using neutron reflection and small unilamellar vesicles (SUVs) using fluorescein leakage and zeta potential changes. The results revealed selective binding to anionic lipid membranes from the lipopeptide and in-membrane nanostructuring that is distinctly different from the co-assembly of the conventional CnTAB. Furthermore, CnTAB binding to the model membranes showed low selectivity, and its high cytotoxicity could be attributed to both membrane lysis and chemical toxicity. This work demonstrates the advantages of the lipopeptides and their potential for further development toward clinical application.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 1520-5827
0743-7463
DOI: 10.1021/acs.langmuir.2c00506
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/35587380
Rights: CC BY
URL: http://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (http://creativecommons.org/licenses/by/4.0/).
Přístupové číslo: edsair.doi.dedup.....7016aab6d2505e4079a56427edf2e420
Databáze: OpenAIRE
Popis
Abstrakt:Cationic biocides have been widely used as active ingredients in personal care and healthcare products for infection control and wound treatment for a long time, but there are concerns over their cytotoxicity and antimicrobial resistance. Designed lipopeptides are potential candidates for alleviating these issues because of their mildness to mammalian host cells and their high efficacy against pathogenic microbial membranes. In this study, antimicrobial and cytotoxic properties of a de novo designed lipopeptide, CH3(CH2)12CO-Lys-Lys-Gly-Gly-Ile-Ile-NH2 (C14KKGGII), were assessed against that of two traditional cationic biocides CnTAB (n = 12 and 14), with different critical aggregation concentrations (CACs). C14KKGGII was shown to be more potent against both bacteria and fungi but milder to fibroblast host cells than the two biocides. Biophysical measurements mimicking the main features of microbial and host cell membranes were obtained for both lipid monolayer models using neutron reflection and small unilamellar vesicles (SUVs) using fluorescein leakage and zeta potential changes. The results revealed selective binding to anionic lipid membranes from the lipopeptide and in-membrane nanostructuring that is distinctly different from the co-assembly of the conventional CnTAB. Furthermore, CnTAB binding to the model membranes showed low selectivity, and its high cytotoxicity could be attributed to both membrane lysis and chemical toxicity. This work demonstrates the advantages of the lipopeptides and their potential for further development toward clinical application.
ISSN:15205827
07437463
DOI:10.1021/acs.langmuir.2c00506