Does Japanese medaka (Oryzias latipes) exhibit a gill Na+/K+-ATPase isoform switch during salinity change?
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| Názov: | Does Japanese medaka (Oryzias latipes) exhibit a gill Na+/K+-ATPase isoform switch during salinity change? |
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| Autori: | Rebecca J. Bollinger, Maryline C. Bossus, Steffen S. Madsen, Christian K. Tipsmark |
| Zdroj: | Bollinger, R J, Madsen, S, Bossus, M & Tipsmark, C K 2016, ' Does Japanese medaka ( Oryzias latipes ) exhibit a gill Na + /K +-ATPase isoform switch during salinity change? ', Journal of Comparative Physiology B, vol. 186, no. 4, pp. 485-501 . https://doi.org/10.1007/s00360-016-0972-6 |
| Informácie o vydavateľovi: | Springer Science and Business Media LLC, 2016. |
| Rok vydania: | 2016 |
| Predmety: | Enzymologic, Fish Proteins, Gills, 0301 basic medicine, Sodium-Potassium-Exchanging ATPase/genetics, Salinity, Acclimatization, Potassium/metabolism, Oryzias, Sequence Homology, Euryhaline teleost, Gene Expression Regulation, Enzymologic, Ouabain/metabolism, α-Subunit, 03 medical and health sciences, Osmoregulation, Na /K -ATPase enzymatic activity, Animals, Magnesium, Fish Proteins/genetics, Ouabain, Phylogeny, 0303 health sciences, Sequence Homology, Amino Acid, Sodium, Substrate affinity, Oryzias/metabolism, Gills/metabolism, Isoenzymes, Amino Acid, Kinetics, Sodium/metabolism, Gene Expression Regulation, Potassium, Magnesium/metabolism, Sodium-Potassium-Exchanging ATPase, Isoenzymes/genetics |
| Popis: | Some euryhaline teleosts exhibit a switch in gill Na(+)/K(+)-ATPase (Nka) α isoform when moving between fresh water (FW) and seawater (SW). The present study tested the hypothesis that a similar mechanism is present in Japanese medaka and whether salinity affects ouabain, Mg(2+), Na(+) and K(+) affinity of the gill enzyme. Phylogenetic analysis classified six separate medaka Nka α isoforms (α1a, α1b, α1c, α2, α3a and α3b). Medaka acclimated long-term (>30 days) to either FW or SW had similar gill expression of α1c, α2, α3a and α3b, while both α1a and α1b were elevated in SW. Since a potential isoform shift may rely on early changes in transcript abundance, we conducted two short-term (1-3 days) salinity transfer experiments. FW to SW acclimation induced an elevation of α1b and α1a after 1 and 3 days. SW to FW acclimation reduced α1b after 3 days with no other α isoforms affected. To verify that the responses were typical, additional transport proteins were examined. Gill ncc and nhe3 expression were elevated in FW, while cftr and nkcc1a were up-regulated in SW. This is in accordance with putative roles in ion-uptake and secretion. SW-acclimated medaka had higher gill Nka V max and lower apparent K m for Na(+) compared to FW fish, while apparent affinities for K(+), Mg(2+) and ouabain were unchanged. The present study showed that the Japanese medaka does not exhibit a salinity-induced α isoform switch and therefore suggests that Na(+) affinity changes involve altered posttranslational modification or intermolecular interactions. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1432-136X 0174-1578 |
| DOI: | 10.1007/s00360-016-0972-6 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/26920794 https://pubmed.ncbi.nlm.nih.gov/26920794/ https://portal.findresearcher.sdu.dk/da/publications/does-japanese-medaka-emoryzias-latipesem-exhibit-a-gill-nasupsupk https://www.infona.pl/resource/bwmeta1.element.springer-doi-10_1007-S00360-016-0972-6 https://link.springer.com/article/10.1007/s00360-016-0972-6 https://link.springer.com/article/10.1007/s00360-016-0972-6/fulltext.html https://www.ncbi.nlm.nih.gov/pubmed/26920794 https://portal.findresearcher.sdu.dk/da/publications/3d0811fb-e28f-4d9b-ae62-a5cd4dfed774 https://doi.org/10.1007/s00360-016-0972-6 https://portal.findresearcher.sdu.dk/da/publications/3d0811fb-e28f-4d9b-ae62-a5cd4dfed774 |
| Rights: | Springer TDM |
| Prístupové číslo: | edsair.doi.dedup.....6ed2793405742f457f87785a05a2ec99 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Some euryhaline teleosts exhibit a switch in gill Na(+)/K(+)-ATPase (Nka) α isoform when moving between fresh water (FW) and seawater (SW). The present study tested the hypothesis that a similar mechanism is present in Japanese medaka and whether salinity affects ouabain, Mg(2+), Na(+) and K(+) affinity of the gill enzyme. Phylogenetic analysis classified six separate medaka Nka α isoforms (α1a, α1b, α1c, α2, α3a and α3b). Medaka acclimated long-term (>30 days) to either FW or SW had similar gill expression of α1c, α2, α3a and α3b, while both α1a and α1b were elevated in SW. Since a potential isoform shift may rely on early changes in transcript abundance, we conducted two short-term (1-3 days) salinity transfer experiments. FW to SW acclimation induced an elevation of α1b and α1a after 1 and 3 days. SW to FW acclimation reduced α1b after 3 days with no other α isoforms affected. To verify that the responses were typical, additional transport proteins were examined. Gill ncc and nhe3 expression were elevated in FW, while cftr and nkcc1a were up-regulated in SW. This is in accordance with putative roles in ion-uptake and secretion. SW-acclimated medaka had higher gill Nka V max and lower apparent K m for Na(+) compared to FW fish, while apparent affinities for K(+), Mg(2+) and ouabain were unchanged. The present study showed that the Japanese medaka does not exhibit a salinity-induced α isoform switch and therefore suggests that Na(+) affinity changes involve altered posttranslational modification or intermolecular interactions. |
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| ISSN: | 1432136X 01741578 |
| DOI: | 10.1007/s00360-016-0972-6 |