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Variation in Cell Wall Composition and Saccharification Potential of Seed‐Based Miscanthus Hybrids Grown on Marginal Lands Across Six European Trial Locations

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Title: Variation in Cell Wall Composition and Saccharification Potential of Seed‐Based Miscanthus Hybrids Grown on Marginal Lands Across Six European Trial Locations
Authors: Kasper van der Cruijsen, Mohamad Al Hassan, Oene Dolstra, Elena Magenau, Mislav Kontek, Chris Ashman, Danny Awty‐Carroll, Andrea Ferrarini, Enrico Martani, Phillip van der Pluijm, Gert‐Jan Petri, Emmanuel de Maupeou, Maria‐João Paulo, Jason Kam, Bert‐Jan van Dinter, Lars Kraak, Annemarie Dechesne, Vanja Juriŝić, Iris Lewandowski, Stefano Amaducci, John Clifton‐Brown, Andreas Kiesel, Luisa M. Trindade
Source: GCB Bioenergy. 17
Publisher Information: Wiley, 2025.
Publication Year: 2025
Subject Terms: biomass quality, cell wall composition, miscanthus, saccharification efficiency, intraspecific and interspecific hybrids, lignocellulosic biofuel
Description: Miscanthus breeding programs have focused on developing intraspecific (M. sinensis × M. sinensis) and interspecific (M. sinensis × M. sacchariflorus) seed‐based hybrids with distinct cell wall characteristics for different biomass value chains. Here, we evaluated the performance of 13 novel hybrids (including seed‐based intraspecific, seed‐based interspecific, and one clonally propagated interspecific hybrid) relative to Miscanthus × giganteus (M × g). We compared the cell wall composition, saccharification efficiency, and yield after spring harvests in 2021 and 2022 across six European locations. Cell wall content and composition varied significantly among hybrids and were influenced by environmental conditions, yet differences due to parental background were largely consistent across locations. On average, seed‐based interspecific hybrids (80.6%–84.0% neutral detergent fiber) had a lower total cell wall content than the other hybrids evaluated in this study (88.3%–90.8%). In contrast, cellulose was ~5.5% higher in hybrids with an M. sinensis × M. sacchariflorus background relative to the intraspecific hybrids, while hemicellulose averaged above 34% for intraspecific hybrids, 29.4% to 31.8% in the interspecific hybrids, and below 27% for M × g. Lignin content was highest in M × g (~13.8%), intermediate in the interspecific hybrids (11.0%–12.2%), and lowest in the intraspecific hybrids (~10%). These compositional traits translated into saccharification efficiencies that were 32.9% higher for the intraspecific hybrids and 9.8%–13.1% higher for the interspecific hybrids (seed‐based and clonally propagated) compared to M × g. Accounting for biomass yield, either several seed‐based hybrids or the novel clonally propagated hybrid exceeded the theoretical ethanol potential of M × g at all trial locations, indicating strong potential for their use in lignocellulosic biofuel production.
Document Type: Article
Language: English
ISSN: 1757-1707
1757-1693
DOI: 10.1111/gcbb.70059
Rights: CC BY
Accession Number: edsair.doi.dedup.....6d96bba88aac4d8abb4d56d2d05fc548
Database: OpenAIRE
Description
Abstract:Miscanthus breeding programs have focused on developing intraspecific (M. sinensis × M. sinensis) and interspecific (M. sinensis × M. sacchariflorus) seed‐based hybrids with distinct cell wall characteristics for different biomass value chains. Here, we evaluated the performance of 13 novel hybrids (including seed‐based intraspecific, seed‐based interspecific, and one clonally propagated interspecific hybrid) relative to Miscanthus × giganteus (M × g). We compared the cell wall composition, saccharification efficiency, and yield after spring harvests in 2021 and 2022 across six European locations. Cell wall content and composition varied significantly among hybrids and were influenced by environmental conditions, yet differences due to parental background were largely consistent across locations. On average, seed‐based interspecific hybrids (80.6%–84.0% neutral detergent fiber) had a lower total cell wall content than the other hybrids evaluated in this study (88.3%–90.8%). In contrast, cellulose was ~5.5% higher in hybrids with an M. sinensis × M. sacchariflorus background relative to the intraspecific hybrids, while hemicellulose averaged above 34% for intraspecific hybrids, 29.4% to 31.8% in the interspecific hybrids, and below 27% for M × g. Lignin content was highest in M × g (~13.8%), intermediate in the interspecific hybrids (11.0%–12.2%), and lowest in the intraspecific hybrids (~10%). These compositional traits translated into saccharification efficiencies that were 32.9% higher for the intraspecific hybrids and 9.8%–13.1% higher for the interspecific hybrids (seed‐based and clonally propagated) compared to M × g. Accounting for biomass yield, either several seed‐based hybrids or the novel clonally propagated hybrid exceeded the theoretical ethanol potential of M × g at all trial locations, indicating strong potential for their use in lignocellulosic biofuel production.
ISSN:17571707
17571693
DOI:10.1111/gcbb.70059