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Changes in tissues and organs through PMCTA carrier substances

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Title: Changes in tissues and organs through PMCTA carrier substances
Authors: G. M. Bruch, N. H. C. Feldmann, F. T. Fischer, T. Fracasso, S. Grabherr, P. Genet
Source: Int J Legal Med
International journal of legal medicine, vol. 139, no. 1, pp. 131-142
Publisher Information: Springer Science and Business Media LLC, 2024.
Publication Year: 2024
Subject Terms: Male, Computed Tomography Angiography, Contrast Media, Middle Aged, Humans, Polyethylene Glycols, Postmortem Changes, Oils, Femoral Vein/diagnostic imaging, Paraffin, Autopsy, Carrier substance, PEG, PMCTA, Paraffin oil, Polyethylenglycole, Immunohistochemistry, Specimen Handling, Female [MeSH], Specimen Handling [MeSH], Humans [MeSH], Oils [MeSH], Middle Aged [MeSH], Immunohistochemistry [MeSH], Original Article, Postmortem Changes [MeSH], Male [MeSH], Polyethylene Glycols [MeSH], Computed Tomography Angiography [MeSH], Contrast Media/adverse effects [MeSH], Paraffin [MeSH], Autopsy [MeSH], Female
Description: To date, lipophilic contrast agents mixed with oil, usually paraffin oil, are the most commonly used contrast agents in post-mortem computed tomography angiography (PMCTA). Iodine-based hydrophilic contrast media in combination with a water-soluble carrier, e.g. polyethylene glycol (PEG), are also common. However, their influence on different tissues and organs is poorly understood. In order to analyse the changes in the cadavers caused by the different carrier substances, we evaluated the effects of PEG 200 and oil on the different tissues and organs. Therefore, during a forensic autopsy, liquid femoral vein blood and samples of different organs and vessels were taken and preserved at room temperature in the two liquids mentioned. The condition of the samples was documented during the autopsy and 24, 48 and 72 h after preservation. Microscopic examination took place after 72 h. After 24 h, the samples placed in PEG 200 already showed a clear solidification of almost all structures. Crumbly blood agglomerates had formed in the previously liquid blood. In contrast, the samples stored in oil showed signs of classic cadaveric decomposition after 24 h, which increased with time. The microscopic and immunohistochemical evaluation of the samples stored in PEG showed a good diagnostic quality. The analysis of tissues stored in oil was much more difficult due to putrefaction. PEG and oil show significantly different effects on human tissues, mainly conservation and dehydration are affected. It is crucial to be aware of these differences in order to choose the most appropriate PMCTA method for each forensic case.
Document Type: Article
Other literature type
File Description: application/pdf
Language: English
ISSN: 1437-1596
0937-9827
DOI: 10.1007/s00414-024-03350-9
Access URL: https://pubmed.ncbi.nlm.nih.gov/39446159
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_9C740044BFE54
https://serval.unil.ch/resource/serval:BIB_9C740044BFE5.P001/REF.pdf
https://serval.unil.ch/notice/serval:BIB_9C740044BFE5
https://repository.publisso.de/resource/frl:6524122
Rights: CC BY
Accession Number: edsair.doi.dedup.....6949e51c7d128813dae71f3c5f4e254f
Database: OpenAIRE
Description
Abstract:To date, lipophilic contrast agents mixed with oil, usually paraffin oil, are the most commonly used contrast agents in post-mortem computed tomography angiography (PMCTA). Iodine-based hydrophilic contrast media in combination with a water-soluble carrier, e.g. polyethylene glycol (PEG), are also common. However, their influence on different tissues and organs is poorly understood. In order to analyse the changes in the cadavers caused by the different carrier substances, we evaluated the effects of PEG 200 and oil on the different tissues and organs. Therefore, during a forensic autopsy, liquid femoral vein blood and samples of different organs and vessels were taken and preserved at room temperature in the two liquids mentioned. The condition of the samples was documented during the autopsy and 24, 48 and 72 h after preservation. Microscopic examination took place after 72 h. After 24 h, the samples placed in PEG 200 already showed a clear solidification of almost all structures. Crumbly blood agglomerates had formed in the previously liquid blood. In contrast, the samples stored in oil showed signs of classic cadaveric decomposition after 24 h, which increased with time. The microscopic and immunohistochemical evaluation of the samples stored in PEG showed a good diagnostic quality. The analysis of tissues stored in oil was much more difficult due to putrefaction. PEG and oil show significantly different effects on human tissues, mainly conservation and dehydration are affected. It is crucial to be aware of these differences in order to choose the most appropriate PMCTA method for each forensic case.
ISSN:14371596
09379827
DOI:10.1007/s00414-024-03350-9