Optimizing Serum RNA Isolation: A Comparative Analysis of Commercial Kits for Yield, Purity, and Contamination Control
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| Názov: | Optimizing Serum RNA Isolation: A Comparative Analysis of Commercial Kits for Yield, Purity, and Contamination Control |
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| Autori: | Esra Duman, Özge Özmen |
| Zdroj: | Volume: 8, Issue: 2229-232 Acta Medica Nicomedia |
| Informácie o vydavateľovi: | Kocaeli University, 2025. |
| Rok vydania: | 2025 |
| Predmety: | RNA Isolation, Serum, A260/280 Ratio, Commercial Reagent Kits, Biyoinformatik ve Hesaplamalı Biyoloji (Diğer), RNA izlasyonu, serum, A260/280 oranı, Ticari Reaktif Kitler, Bioinformatics and Computational Biology (Other) |
| Popis: | Objective: Isolation of RNA from serum samples has gained importance, especially in studies on the use of small RNA molecules such as miRNA as biomarkers. Selection of the optimal kit is critical for the accuracy of downstream processing. The aim of this study was to compare the performance of different commercial kits in terms of efficiency, RNA purity and contamination control during the isolation process. Methods: Three different RNA isolation kits were used for 5 serum samples: 1.miRNeasy Serum/Plasma Kit (Cat. No: 217184, Qiagen, USA), 2.Norgen Plasma/serum RNA purification kit (Cat. No: 55000, Norgen, Canada), 3.Nucleogene RNA isolation kit (Cat. No: NG044, Nucleogene, Turkey). The purity and intensity of the obtained RNAs were evaluated by measuring A260/280 ratios with a nanodrop spectrophotometer. Results: When the concentrations and A260/280 ratios obtained from the kits were evaluated by One Way Anova Test using GraphPad Prism (V10.4.0), it was observed that there was a statistically significant difference between the concentrations and A260/280 ratios of the 3 kits (p≤0.05 and p≤ 0.001). RNAs obtained from Norgene had the lowest concentration and the lowest A260/280 ratio, while Nucleogene had the highest RNA concentration and A260/280 ratio of 2 and above among the three kits (p≤0.05). |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| ISSN: | 2717-8994 |
| DOI: | 10.53446/actamednicomedia.1628412 |
| Prístupová URL adresa: | https://avesis.kocaeli.edu.tr/publication/details/9365cb6d-16d4-409a-9153-57d6cbc23df9/oai https://dergipark.org.tr/tr/pub/actamednicomedia/issue/93373/1628412 |
| Prístupové číslo: | edsair.doi.dedup.....673d90ba27e93eb66d90b6a3f795c0f1 |
| Databáza: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Objective: Isolation of RNA from serum samples has gained importance, especially in studies on the use of small RNA molecules such as miRNA as biomarkers. Selection of the optimal kit is critical for the accuracy of downstream processing. The aim of this study was to compare the performance of different commercial kits in terms of efficiency, RNA purity and contamination control during the isolation process. Methods: Three different RNA isolation kits were used for 5 serum samples: 1.miRNeasy Serum/Plasma Kit (Cat. No: 217184, Qiagen, USA), 2.Norgen Plasma/serum RNA purification kit (Cat. No: 55000, Norgen, Canada), 3.Nucleogene RNA isolation kit (Cat. No: NG044, Nucleogene, Turkey). The purity and intensity of the obtained RNAs were evaluated by measuring A260/280 ratios with a nanodrop spectrophotometer. Results: When the concentrations and A260/280 ratios obtained from the kits were evaluated by One Way Anova Test using GraphPad Prism (V10.4.0), it was observed that there was a statistically significant difference between the concentrations and A260/280 ratios of the 3 kits (p≤0.05 and p≤ 0.001). RNAs obtained from Norgene had the lowest concentration and the lowest A260/280 ratio, while Nucleogene had the highest RNA concentration and A260/280 ratio of 2 and above among the three kits (p≤0.05). |
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| ISSN: | 27178994 |
| DOI: | 10.53446/actamednicomedia.1628412 |