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Capillary Electrophoretic Characterization of Water-soluble Carbon Nanodots Formed from Glutamic Acid and Boric Acid under Microwave Irradiation

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Title: Capillary Electrophoretic Characterization of Water-soluble Carbon Nanodots Formed from Glutamic Acid and Boric Acid under Microwave Irradiation
Authors: Takayanagi, Toshio, Iwasaki, Sota, Becchaku, Yuta, Yabe, Shun, Morita, Kotaro, Mizuguchi, Hitoshi, Hirayama, Naoki
Source: Analytical Sciences. 36:941-946
Publisher Information: Springer Science and Business Media LLC, 2020.
Publication Year: 2020
Subject Terms: 03 medical and health sciences, 0302 clinical medicine, capillary electrophoresis, micellar electrokinetic chromatography, characterization, affinity capillary electrophoresis, Carbon nanodot
Description: Water-soluble carbon nanodots (CND) were synthesized under microwave irradiation from glutamic acid or glutamic acid-boric acid mixture. The CNDs were collected in an aqueous solution through size fractionation by centrifugal filtration. The CNDs thus prepared were subjected to characterization by capillary electrophoresis (CE). A peak signal of anionic substance was detected in the electropherogram, and it was found to be a major component of the CNDs. The effective electrophoretic mobility of the major component was almost identical over the pH range between 6.7 and 11.6, suggesting that the functional group of amine or boric acid moiety was not included in the CNDs. The effective electrophoretic mobility decreased at an acidic pH of less than 5, and it was suggested that carboxylate moiety was included in the CNDs. A signal of less-charged CNDs was also detected in the electropherogram, and the CNDs were characterized by a CE format of micellar electrokinetic chromatography. Two or four peaks were detected just after the electroosmotic flow; the less-charged CNDs were thus hydrophilic. The affinity interaction was also examined between the major anionic CNDs and a hydrophobic pairing cation. The peak signal of the major anionic CNDs broadened, and its theoretical number of plates decreased in the presence of tetrabutylammonium ion in the separation buffer. A small portion of the anionic CNDs were a little hydrophobic at different degrees, and their effective electrophoretic mobility decreased by the hydrophobic interaction, resulting in peak broadening of the anionic CNDs.
Document Type: Article
Language: English
ISSN: 1348-2246
0910-6340
DOI: 10.2116/analsci.19p484
Access URL: https://www.jstage.jst.go.jp/article/analsci/36/8/36_19P484/_pdf
https://pubmed.ncbi.nlm.nih.gov/32009023
https://www.jstage.jst.go.jp/article/analsci/36/8/36_19P484/_article
https://repo.lib.tokushima-u.ac.jp/files/public/11/114230/20200817093214912685/analsci_36_8_941.pdf
https://www.ncbi.nlm.nih.gov/pubmed/32009023
https://europepmc.org/article/MED/32009023
https://repo.lib.tokushima-u.ac.jp/114230
Rights: Springer TDM
Accession Number: edsair.doi.dedup.....671e42a321c48daf47a4a243c9954e5b
Database: OpenAIRE
Description
Abstract:Water-soluble carbon nanodots (CND) were synthesized under microwave irradiation from glutamic acid or glutamic acid-boric acid mixture. The CNDs were collected in an aqueous solution through size fractionation by centrifugal filtration. The CNDs thus prepared were subjected to characterization by capillary electrophoresis (CE). A peak signal of anionic substance was detected in the electropherogram, and it was found to be a major component of the CNDs. The effective electrophoretic mobility of the major component was almost identical over the pH range between 6.7 and 11.6, suggesting that the functional group of amine or boric acid moiety was not included in the CNDs. The effective electrophoretic mobility decreased at an acidic pH of less than 5, and it was suggested that carboxylate moiety was included in the CNDs. A signal of less-charged CNDs was also detected in the electropherogram, and the CNDs were characterized by a CE format of micellar electrokinetic chromatography. Two or four peaks were detected just after the electroosmotic flow; the less-charged CNDs were thus hydrophilic. The affinity interaction was also examined between the major anionic CNDs and a hydrophobic pairing cation. The peak signal of the major anionic CNDs broadened, and its theoretical number of plates decreased in the presence of tetrabutylammonium ion in the separation buffer. A small portion of the anionic CNDs were a little hydrophobic at different degrees, and their effective electrophoretic mobility decreased by the hydrophobic interaction, resulting in peak broadening of the anionic CNDs.
ISSN:13482246
09106340
DOI:10.2116/analsci.19p484