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Multi-level engineering of Baeyer-Villiger monooxygenase-based Escherichia coli biocatalysts for the production of C9 chemicals from oleic acid

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Název: Multi-level engineering of Baeyer-Villiger monooxygenase-based Escherichia coli biocatalysts for the production of C9 chemicals from oleic acid
Autoři: Seo, E.-J., Kang, C.W., Woo, J.-M., Jang, S., Yeon, Y.J., Jung, G.Y., Park, J.-B.
Přispěvatelé: Kang, C.W., Jung, G.Y.
Zdroj: Metabolic Engineering. 54:137-144
Informace o vydavateli: Elsevier BV, 2019.
Rok vydání: 2019
Témata: 0106 biological sciences, 0301 basic medicine, host cell, biocatalyst, nonanoic acid, Whole-cell biotransformations, Baeyer–Villiger monooxygenase, Biochemistry, 01 natural sciences, Mixed Function Oxygenases, oxidative stress, Copy number, n nonanoic acid, Gene expression control, Fatty Acids, alcohol dehydrogenase, Enzymes, unclassified drug, 3. Good health, protein expression level, priority journal, Metabolic Engineering, medium chain fatty acid, Secondary alcohol dehydrogenase, Baeyer-Villiger monooxygenases, Biotransformation pathways, Catalysis, Article, whole cell, 03 medical and health sciences, temperature stress, Bacterial Proteins, plasmid, Escherichia coli, Plasmid copy number, Saturated fatty acids, Fatty acids, nonhuman, Pseudomonas putida, Cell engineering, DNA, Monooxygenases, unspecific monooxygenase, Baeyer Villiger reaction, Oleic acid, Expression controls, Biocatalysis, 9 hydroxynonanoic acid, Gene expression, Cytology, Oleic Acid
Popis: Whole-cell biotransformation is one of the promising alternative approaches to microbial fermentation for producing high-value chemicals. Baeyer-Villiger monooxygenase (BVMO)-based Escherichia coli biocatalysts have been engineered to produce industrially relevant C9 chemicals, such as n-nonanoic acid and 9-hydroxynonanoic acid, from a renewable long-chain fatty acid. The key enzyme in the biotransformation pathway (i.e., BVMO from Pseudomonans putida KT2440) was first engineered, using structure modeling-based design, to improve oxidative and thermal stabilities. Using a stable and tunable plasmid (STAPL) system, E. coli host cells were engineered to have increased plasmid stability and homogeneity of the recombinant E. coli population, as well as to optimize the level of BVMO expression. Multi-level engineering of the key enzyme in host cells, allowed recombinant E. coli expressing a fatty acid double-bond hydratase, a long-chain secondary alcohol dehydrogenase, and the engineered BVMO from P. putida KT2440 (i.e., E6BVMO_C302L/M340L), to ultimately produce C9 chemicals (i.e., n-nonanoic acid and 9-hydroxynonanoic acid) from oleic acid, with a yield of up to 6 mmoL/g dry cells. This yield was 2.4-fold greater than the yield in the control strain before engineering. Therefore, this study will contribute to the development of improved processes for the biosynthesis of industrially relevant medium chain fatty acids via whole-cell biocatalysis.
Druh dokumentu: Article
Jazyk: English
ISSN: 1096-7176
DOI: 10.1016/j.ymben.2019.03.012
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/30953778
https://dspace.ewha.ac.kr/handle/2015.oak/249711
https://www.sciencedirect.com/science/article/pii/S1096717619300989
https://www.ncbi.nlm.nih.gov/pubmed/30953778
https://europepmc.org/article/MED/30953778
https://pubmed.ncbi.nlm.nih.gov/30953778/
https://oasis.postech.ac.kr/handle/2014.oak/99336
Rights: Elsevier TDM
Přístupové číslo: edsair.doi.dedup.....66330cd759a38f2694098bd76c4cb8b8
Databáze: OpenAIRE
Popis
Abstrakt:Whole-cell biotransformation is one of the promising alternative approaches to microbial fermentation for producing high-value chemicals. Baeyer-Villiger monooxygenase (BVMO)-based Escherichia coli biocatalysts have been engineered to produce industrially relevant C9 chemicals, such as n-nonanoic acid and 9-hydroxynonanoic acid, from a renewable long-chain fatty acid. The key enzyme in the biotransformation pathway (i.e., BVMO from Pseudomonans putida KT2440) was first engineered, using structure modeling-based design, to improve oxidative and thermal stabilities. Using a stable and tunable plasmid (STAPL) system, E. coli host cells were engineered to have increased plasmid stability and homogeneity of the recombinant E. coli population, as well as to optimize the level of BVMO expression. Multi-level engineering of the key enzyme in host cells, allowed recombinant E. coli expressing a fatty acid double-bond hydratase, a long-chain secondary alcohol dehydrogenase, and the engineered BVMO from P. putida KT2440 (i.e., E6BVMO_C302L/M340L), to ultimately produce C9 chemicals (i.e., n-nonanoic acid and 9-hydroxynonanoic acid) from oleic acid, with a yield of up to 6 mmoL/g dry cells. This yield was 2.4-fold greater than the yield in the control strain before engineering. Therefore, this study will contribute to the development of improved processes for the biosynthesis of industrially relevant medium chain fatty acids via whole-cell biocatalysis.
ISSN:10967176
DOI:10.1016/j.ymben.2019.03.012