Diversity and Pathobiology of an Ilarvirus Unexpectedly Detected in Diverse Plants and Global Sequencing Data
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| Název: | Diversity and Pathobiology of an Ilarvirus Unexpectedly Detected in Diverse Plants and Global Sequencing Data |
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| Autoři: | Rivarez, Mark Paul Selda, Faure, Chantal, Svanella-Dumas, Laurence, Pecman, Anja, Tušek-Žnidaric, Magda, Schönegger, Deborah, De Jonghe, Kris, Blouin, Arnaud, Rasmussen, David A, Massart, Sébastien, Ravnikar, Maja, Kutnjak, Denis, Marais, Armelle, Candresse, Thierry |
| Přispěvatelé: | EU - European Union, FPS Health Federal Public Service Health, Food Chain Safety and Environment, Gauthier, Muriel, National Institute of Biology Ljubljana (NIB), North Carolina State University Raleigh (NC State), University of North Carolina System (UNC), Caraga State University, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Flanders Research Institute for Agriculture, TERRA, Gembloux Agro-Bio Tech, Gembloux Agro-Bio Tech Faculté universitaire des sciences agronomiques de Gembloux ( FUSAGx ), Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège), Agroscope, This work mainly received funding from Horizon 2020 Marie Skłodowska-Curie Actions Innovative Training Network (MSCA-ITN) project 'Innovative Network for Next Generation Training and Sequencing of Virome (INEXTVIR)' (GA 813542) under the managementof the European Commission-Research Executive Agency. It was also supported by the funding from Slovenian Research Agency (ARRS) financing (P4-0165, P4-0407, J4-4553). Funding for the work in Belgium was provided by The Belgian FPS Health Food Chain Safety and Environment under Project RT18/3 SEVIPLANT. MPS Rivarez also received funding from the BalikScientist Program (Republic Act 11035) of the Department of Science and Technology –Philippine Council for Agriculture, Aquatic, and Natural Resources Research and Development (DOST–PCAARRD), Republic of the Philippines, during the manuscript writing and submission., European Project |
| Zdroj: | Phytopathology®. 113:1729-1744 |
| Informace o vydavateli: | Scientific Societies, 2023. |
| Rok vydání: | 2023 |
| Témata: | Nicotiana, [SDV]Life Sciences [q-bio], virus diversity, Serratus, Plant Science, Ilarvirus, Biochimie, biophysique & biologie moléculaire, Solanum, Tobacco, filogenetika, virion morphology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Solanaceae, Phylogeny, Plant Diseases, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, virus transmission, Life sciences, 3. Good health, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV] Life Sciences [q-bio], phylogenetics, pollen, symptomatology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, histopathology, Sciences du vivant, cvetni prah, Agronomy and Crop Science, histopatologija, Biochemistry, biophysics & molecular biology |
| Popis: | High-throughput sequencing (HTS) and sequence mining tools revolutionized virus detection and discovery in recent years, and implementing them with classical plant virology techniques results in a powerful approach to characterize viruses. An example of a virus discovered through HTS is Solanum nigrum ilarvirus 1 (SnIV1) ( Bromoviridae), which was recently reported in various solanaceous plants from France, Slovenia, Greece, and South Africa. It was likewise detected in grapevines ( Vitaceae) and several Fabaceae and Rosaceae plant species. Such a diverse set of source organisms is atypical for ilarviruses, thus warranting further investigation. In this study, modern and classical virological tools were combined to accelerate the characterization of SnIV1. Through HTS-based virome surveys, mining of sequence read archive datasets, and a literature search, SnIV1 was further identified from diverse plant and non-plant sources globally. SnIV1 isolates showed relatively low variability compared with other phylogenetically related ilarviruses. Phylogenetic analyses showed a distinct basal clade of isolates from Europe, whereas the rest formed clades of mixed geographic origin. Furthermore, systemic infection of SnIV1 in Solanum villosum and its mechanical and graft transmissibility to solanaceous species were demonstrated. Near-identical SnIV1 genomes from the inoculum ( S. villosum) and inoculated Nicotiana benthamiana were sequenced, thus partially fulfilling Koch's postulates. SnIV1 was shown to be seed-transmitted and potentially pollen-borne, has spherical virions, and possibly induces histopathological changes in infected N. benthamiana leaf tissues. Overall, this study provides information to better understand the diversity, global presence, and pathobiology of SnIV1; however, its possible emergence as a destructive pathogen remains uncertain. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license . |
| Druh dokumentu: | Article |
| Popis souboru: | text/url; application/pdf |
| Jazyk: | English |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-12-22-0465-v |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/37399026 https://hdl.handle.net/20.500.12556/DiRROS-19260 https://doi.org/10.1094/phyto-12-22-0465-v https://dirros.openscience.si/IzpisGradiva.php?id=19260 https://hal.inrae.fr/hal-04215253v1 https://doi.org/10.1094/phyto-12-22-0465-v https://hal.inrae.fr/hal-04215253v1/document |
| Rights: | CC BY |
| Přístupové číslo: | edsair.doi.dedup.....64ff95ace169b5e69d8e36f41c3e3ca6 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | High-throughput sequencing (HTS) and sequence mining tools revolutionized virus detection and discovery in recent years, and implementing them with classical plant virology techniques results in a powerful approach to characterize viruses. An example of a virus discovered through HTS is Solanum nigrum ilarvirus 1 (SnIV1) ( Bromoviridae), which was recently reported in various solanaceous plants from France, Slovenia, Greece, and South Africa. It was likewise detected in grapevines ( Vitaceae) and several Fabaceae and Rosaceae plant species. Such a diverse set of source organisms is atypical for ilarviruses, thus warranting further investigation. In this study, modern and classical virological tools were combined to accelerate the characterization of SnIV1. Through HTS-based virome surveys, mining of sequence read archive datasets, and a literature search, SnIV1 was further identified from diverse plant and non-plant sources globally. SnIV1 isolates showed relatively low variability compared with other phylogenetically related ilarviruses. Phylogenetic analyses showed a distinct basal clade of isolates from Europe, whereas the rest formed clades of mixed geographic origin. Furthermore, systemic infection of SnIV1 in Solanum villosum and its mechanical and graft transmissibility to solanaceous species were demonstrated. Near-identical SnIV1 genomes from the inoculum ( S. villosum) and inoculated Nicotiana benthamiana were sequenced, thus partially fulfilling Koch's postulates. SnIV1 was shown to be seed-transmitted and potentially pollen-borne, has spherical virions, and possibly induces histopathological changes in infected N. benthamiana leaf tissues. Overall, this study provides information to better understand the diversity, global presence, and pathobiology of SnIV1; however, its possible emergence as a destructive pathogen remains uncertain. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license . |
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| ISSN: | 19437684 0031949X |
| DOI: | 10.1094/phyto-12-22-0465-v |