Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses
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| Title: | Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses |
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| Authors: | Verhaar, Nicole, de Buhr, Nicole, von Köckritz-Blickwede, Maren, Hewicker-Trautwein, Marion, Pfarrer, Christiane, Mazzuoli-Weber, Gemma, Schulte, Henri, Kästner, Sabine |
| Source: | BMC Vet Res BMC Veterinary Research, Vol 17, Iss 1, Pp 1-14 (2021) |
| Publisher Information: | Springer Science and Business Media LLC, 2021. |
| Publication Year: | 2021 |
| Subject Terms: | 0301 basic medicine, 0303 health sciences, Superoxide Dismutase, Veterinary medicine, Research, Apoptosis, 3. Good health, 03 medical and health sciences, Jejunum, Malondialdehyde, Reperfusion Injury, SF600-1100, Animals, HSP70 Heat-Shock Proteins, Ischemic Postconditioning/methods [MeSH], HSP70 Heat-Shock Proteins/metabolism [MeSH], Reperfusion Injury/veterinary [MeSH], Horses [MeSH], Animals [MeSH], Apoptosis [MeSH], Veterinary Medicine/Veterinary Science, Lactic Acid/blood [MeSH], Intestinal Mucosa/metabolism [MeSH], Jejunum/blood supply [MeSH], Zoology, Malondialdehyde/metabolism [MeSH], Ischemic Postconditioning/veterinary [MeSH], Jejunum/pathology [MeSH], Transgenics, Superoxide Dismutase/metabolism [MeSH], Reperfusion Injury/therapy [MeSH], Horses, Lactic Acid, Intestinal Mucosa, Ischemic Postconditioning |
| Description: | BackgroundIschaemic postconditioning (IPoC) refers to brief periods of reocclusion of blood supply following an ischaemic event. This has been shown to ameliorate ischaemia reperfusion injury in different tissues, and it may represent a feasible therapeutic strategy for ischaemia reperfusion injury following strangulating small intestinal lesions in horses. The objective of this study was to assess the degree cell death, inflammation, oxidative stress, and heat shock response in an equine experimental jejunal ischaemia model with and without IPoC.MethodsIn this randomized, controlled, experimental in vivo study, 14 horses were evenly assigned to a control group and a group subjected to IPoC. Under general anaesthesia, segmental ischaemia with arterial and venous occlusion was induced in 1.5 m jejunum. Following ischaemia, the mesenteric vessels were repeatedly re-occluded in group IPoC only. Full thickness intestinal samples and blood samples were taken at the end of the pre-ischaemia period, after ischaemia, and after 120 min of reperfusion. Immunohistochemical staining or enzymatic assays were performed to determine the selected variables.ResultsThe mucosal cleaved-caspase-3 and TUNEL cell counts were significantly increased after reperfusion in the control group only. The cleaved-caspase-3 cell count was significantly lower in group IPoC after reperfusion compared to the control group. After reperfusion, the tissue myeloperoxidase activity and the calprotectin positive cell counts in the mucosa were increased in both groups, and only group IPoC showed a significant increase in the serosa. Tissue malondialdehyde and superoxide dismutase as well as blood lactate levels showed significant progression during ischaemia or reperfusion. The nuclear immunoreactivity of Heat shock protein-70 increased significantly during reperfusion. None of these variables differed between the groups. The neuronal cell counts in the myenteric plexus ganglia were not affected by the ischaemia model.ConclusionsA reduced apoptotic cell count was found in the group subjected to IPoC. None of the other tested variables were significantly affected by IPoC. Therefore, the clinical relevance and possible protective mechanism of IPoC in equine intestinal ischaemia remains unclear. Further research on the mechanism of action and its effect in clinical cases of strangulating colic is needed. |
| Document Type: | Article Conference object Other literature type |
| Language: | English |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-021-02877-y |
| Access URL: | https://bmcvetres.biomedcentral.com/track/pdf/10.1186/s12917-021-02877-y https://pubmed.ncbi.nlm.nih.gov/33902575 https://doaj.org/article/5722b288455547aa89312c87609373be https://europepmc.org/article/MED/33902575 https://link.springer.com/article/10.1186/s12917-021-02877-y https://elib.tiho-hannover.de/receive/tiho_mods_00004811 https://link.springer.com/article/10.1186/s12917-021-02877-y/figures/1 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-021-02877-y https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077964 https://repository.publisso.de/resource/frl:6462925 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....63b7525041492569dc03399350a5ee1a |
| Database: | OpenAIRE |
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| Abstract: | BackgroundIschaemic postconditioning (IPoC) refers to brief periods of reocclusion of blood supply following an ischaemic event. This has been shown to ameliorate ischaemia reperfusion injury in different tissues, and it may represent a feasible therapeutic strategy for ischaemia reperfusion injury following strangulating small intestinal lesions in horses. The objective of this study was to assess the degree cell death, inflammation, oxidative stress, and heat shock response in an equine experimental jejunal ischaemia model with and without IPoC.MethodsIn this randomized, controlled, experimental in vivo study, 14 horses were evenly assigned to a control group and a group subjected to IPoC. Under general anaesthesia, segmental ischaemia with arterial and venous occlusion was induced in 1.5 m jejunum. Following ischaemia, the mesenteric vessels were repeatedly re-occluded in group IPoC only. Full thickness intestinal samples and blood samples were taken at the end of the pre-ischaemia period, after ischaemia, and after 120 min of reperfusion. Immunohistochemical staining or enzymatic assays were performed to determine the selected variables.ResultsThe mucosal cleaved-caspase-3 and TUNEL cell counts were significantly increased after reperfusion in the control group only. The cleaved-caspase-3 cell count was significantly lower in group IPoC after reperfusion compared to the control group. After reperfusion, the tissue myeloperoxidase activity and the calprotectin positive cell counts in the mucosa were increased in both groups, and only group IPoC showed a significant increase in the serosa. Tissue malondialdehyde and superoxide dismutase as well as blood lactate levels showed significant progression during ischaemia or reperfusion. The nuclear immunoreactivity of Heat shock protein-70 increased significantly during reperfusion. None of these variables differed between the groups. The neuronal cell counts in the myenteric plexus ganglia were not affected by the ischaemia model.ConclusionsA reduced apoptotic cell count was found in the group subjected to IPoC. None of the other tested variables were significantly affected by IPoC. Therefore, the clinical relevance and possible protective mechanism of IPoC in equine intestinal ischaemia remains unclear. Further research on the mechanism of action and its effect in clinical cases of strangulating colic is needed. |
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| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-021-02877-y |