Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
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| Title: | Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories |
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| Authors: | Lara Chuvala, Alexandria E Kesterson, Henry S. Heine, John E Craig |
| Source: | Emerg Infect Dis Emerging Infectious Diseases, Vol 26, Iss 11, Pp 2586-2590 (2020) |
| Publisher Information: | Centers for Disease Control and Prevention (CDC), 2020. |
| Publication Year: | 2020 |
| Subject Terms: | filtration, 0301 basic medicine, 0303 health sciences, Burkholderia pseudomallei, select agents, Yersinia pestis, sterility, Research, Sterilization, Infectious and parasitic diseases, RC109-216, Containment of Biohazards, biosafety level 3, Burkholderia mallei, BSL-3, 3. Good health, 03 medical and health sciences, Bacillus anthracis, Medicine, Francisella tularensis, Laboratories, methanol |
| Description: | The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories. |
| Document Type: | Article Other literature type |
| ISSN: | 1080-6059 1080-6040 |
| DOI: | 10.3201/eid2611.191630 |
| Access URL: | https://wwwnc.cdc.gov/eid/article/26/11/pdfs/19-1630.pdf https://pubmed.ncbi.nlm.nih.gov/33079040 https://doaj.org/article/9c1396b5d3484555baebac338abae414 https://europepmc.org/article/PMC/PMC7588557 https://pubmed.ncbi.nlm.nih.gov/33079040/ https://www.ncbi.nlm.nih.gov/pubmed/33079040 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588557 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....629feb98bfd56824e0d94148002ff8b2 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories. |
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| ISSN: | 10806059 10806040 |
| DOI: | 10.3201/eid2611.191630 |