Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth
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| Název: | Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth |
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| Autoři: | Ivarsen, Elise, Fretté, Xavier, Christensen, Kathrine Bisgaard, Christensen, Lars Porskjær, Engberg, Ricarda M, Grevsen, Kai, Kjær, Anders |
| Zdroj: | Ivarsen, E, Fretté, X, Christensen, K B, Christensen, L P, Engberg, R G, Grevsen, K & Kjær, A 2015, ' Bioassay-guided chromatographic isolation and identification of antibacterial compounds from Artemisia annua L. that inhibit Clostridium perfringens growth ', Journal of AOAC International, vol. 97, no. 5, pp. 1282-1290 . https://doi.org/10.5740/jaoacint.SGEIvarsen Ivarsen, E, Fretté, X, Christensen, K B, Christensen, L P, Engberg, R M, Grevsen, K & Kjær, A 2014, 'Bioassay-guided chromatographic isolation and identification of antibacterial compounds from Artemisia annua L. That inhibit Clostridium perfringens growth', Journal of AOAC International, vol. 97, no. 5, pp. 1282-1290. https://doi.org/10.5740/jaoacint.SGEIvarsen |
| Informace o vydavateli: | Oxford University Press (OUP), 2014. |
| Rok vydání: | 2014 |
| Témata: | 0301 basic medicine, Chromatography, 0303 health sciences, Clostridium perfringens, Plant Extracts, Clostridium perfringens/drug effects, Anti-Bacterial Agents/chemistry, Microbial Sensitivity Tests, Plant Extracts/analysis, Artemisia annua, Anti-Bacterial Agents, 3. Good health, 03 medical and health sciences, High Pressure Liquid/methods, Biological Assay, Artemisia annua/metabolism, Chromatography, High Pressure Liquid/methods, Chromatography, High Pressure Liquid |
| Popis: | Clostridium perfringens is the causative agent of necrotic enteritis leading to significant losses in the poultry industry. Dichloromethane and n-hexane extracts of aerial parts of Artemisia annua (Asteraceae) exhibited activity against C. perfringens with minimum inhibitory concentrations (MIC) of 185 and 270 μg/mL, respectively. Bioassay-guided fractionation of the extracts gave several active fractions (MIC between 75 and 600 μg/mL). Investigations of the most active fractions resulted in the iso ponticaepoxide (MIC between 100 and 200 μg/mL) and (+)-threo-(5E)-trideca-1,5-dien-7,9,11-triyne-3,4-diol (MIC between 400 and 800 μg/mL), the flavonols chrysosplenol D (MIC between 200 and 400 μg/mL) and casticin (slight inhibition at 800 μg/mL), and 2,4-dihydroxy-6-methoxyacetophenone (slight inhibition at 800 μg/mL). Also, the coumarin scopoletin and the sesquiterpene lactone artemisinin were isolated from active fractions but showed no inhibition of C. perfringens growth at 800 and 2000 μg/mL, respectively. Fractions containing essential oil components with camphor constituting >60% did not show inhibition of C. perfringens up to 1600 μg/mL. Extracts and some active fractions showed higher antibacterial effect than individual bioactive compounds, suggesting that synergistic effects may underlie the observed antibacterial effect. The present study adds new valuable information on the antibacterial effect of A. annua against C. perfringens. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.sgeivarsen |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/25902977 https://www.ncbi.nlm.nih.gov/pubmed/25902977 https://academic.oup.com/jaoac/article/97/5/1282/5654867 http://findresearcher.sdu.dk/portal/da/publications/bioassayguided-chromatographic-isolation-and-identification-of-antibacterial-compounds-from-artemisia-annua-l-that-inhibit-clostridium-perfringens-growth(ab646f4c-7eb4-4060-8191-070b0a640efb)/export.html http://europepmc.org/abstract/MED/25902977 https://vbn.aau.dk/da/publications/bioassay-guided-chromatographic-isolation-and-identification-of-a https://portal.findresearcher.sdu.dk/da/publications/ab646f4c-7eb4-4060-8191-070b0a640efb |
| Rights: | OUP Standard Publication Reuse |
| Přístupové číslo: | edsair.doi.dedup.....5d01aab8713062824be60a0a0408cef4 |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Clostridium perfringens is the causative agent of necrotic enteritis leading to significant losses in the poultry industry. Dichloromethane and n-hexane extracts of aerial parts of Artemisia annua (Asteraceae) exhibited activity against C. perfringens with minimum inhibitory concentrations (MIC) of 185 and 270 μg/mL, respectively. Bioassay-guided fractionation of the extracts gave several active fractions (MIC between 75 and 600 μg/mL). Investigations of the most active fractions resulted in the iso ponticaepoxide (MIC between 100 and 200 μg/mL) and (+)-threo-(5E)-trideca-1,5-dien-7,9,11-triyne-3,4-diol (MIC between 400 and 800 μg/mL), the flavonols chrysosplenol D (MIC between 200 and 400 μg/mL) and casticin (slight inhibition at 800 μg/mL), and 2,4-dihydroxy-6-methoxyacetophenone (slight inhibition at 800 μg/mL). Also, the coumarin scopoletin and the sesquiterpene lactone artemisinin were isolated from active fractions but showed no inhibition of C. perfringens growth at 800 and 2000 μg/mL, respectively. Fractions containing essential oil components with camphor constituting >60% did not show inhibition of C. perfringens up to 1600 μg/mL. Extracts and some active fractions showed higher antibacterial effect than individual bioactive compounds, suggesting that synergistic effects may underlie the observed antibacterial effect. The present study adds new valuable information on the antibacterial effect of A. annua against C. perfringens. |
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| ISSN: | 19447922 10603271 |
| DOI: | 10.5740/jaoacint.sgeivarsen |