Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
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| Title: | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
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| Authors: | Gajović, Srećko, Gorup, Dunja, Erjavec, Igor, Bohaček, Ivan, Dobrivojević, Marina |
| Source: | Croat Med J Croatian Medical Journal CODEN CMEJEN Volume 54 Issue 1 |
| Publisher Information: | Croatian Medical Journals, 2013. |
| Publication Year: | 2013 |
| Subject Terms: | Male, 0301 basic medicine, Contrast Media/diagnostic use, Iohexol, Contrast Media, Brain Ischemia/pathology, Brain Edema, Brain Ischemia, Mice, 03 medical and health sciences, 0302 clinical medicine, Infarction, Middle Cerebral Artery/pathology, Animals, Iothalamic Acid/diagnostic use, Coloring Agents, Infarction, Middle Cerebral Artery/radiography, stroke, micro CT, contrast, Iohexol/diagnostic use, Staining and Labeling, Brain Edema/pathology, Infarction, Middle Cerebral Artery, X-Ray Microtomography, Clinical Science, Iothalamic Acid, 3. Good health, Mice, Inbred C57BL, Disease Models, Animal, Brain Ischemia/radiography, Iothalamic Acid/analogs & derivatives, Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
| Description: | To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO).Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed.Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity.MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema. |
| Document Type: | Article Other literature type |
| File Description: | application/pdf |
| ISSN: | 1332-8166 0353-9504 |
| DOI: | 10.3325/cmj.2013.54.3 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/23444240 https://www.bib.irb.hr/741415 http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=151286 https://hrcak.srce.hr/file/151286 http://europepmc.org/articles/PMC3584505 https://www.bib.irb.hr/741415 https://hrcak.srce.hr/102765 http://hrcak.srce.hr/file/151286 https://core.ac.uk/display/17339118 https://urn.nsk.hr/urn:nbn:hr:105:199163 https://doi.org/10.3325/cmj.2013.54.3 https://hrcak.srce.hr/102765 https://hrcak.srce.hr/file/151286 https://hrcak.srce.hr/102765 |
| Rights: | CC BY NC ND CC BY URL: http://rightsstatements.org/vocab/InC/1.0/ |
| Accession Number: | edsair.doi.dedup.....5cbbaa413404eb50e3a83df90767e126 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO).Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed.Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity.MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema. |
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| ISSN: | 13328166 03539504 |
| DOI: | 10.3325/cmj.2013.54.3 |