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Colorimetry-based quantification of protein droplets using 'ImageJ'

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Bibliographic Details
Title: Colorimetry-based quantification of protein droplets using 'ImageJ'
Authors: Sulaiman Ngongu Depamede, Djoko Kisworo
Source: GSC Biological and Pharmaceutical Sciences. 29:431-434
Publisher Information: GSC Online Press, 2024.
Publication Year: 2024
Subject Terms: Bovine serum albumin, Ovalbumin, Protein quantification, Smartphone
Description: The objective of this study is to analyze protein concentration on a colorimetric basis by utilizing the openly accessible 'ImageJ' software. We herein report the successful measurement of protein concentration on a parafilm surface, following the application of Bradford reagent and subsequent photography with a smartphone, followed by analysis using the ImageJ software. Ovalbumin (OVA) and bovine serum albumin (BSA) were dissolved in phosphate buffered saline (PBS) and used as standards. The results demonstrated a linear correlation between integrated density and the actual weight of the standard, with R² values of 0.9908 for OVA samples in the range of 0-1.5 mg/ml and 0.9781 for BSA in the range of 0.25-1.5 mg/ml. The straightforward nature of the experiments and analyses makes the study we present here an invaluable resource for researchers and students who are constrained by equipment limitations, such as the lack of access to spectrophotometers.
Document Type: Article
ISSN: 2581-3250
DOI: 10.30574/gscbps.2024.29.2.0426
DOI: 10.5281/zenodo.14821850
DOI: 10.5281/zenodo.14821851
Rights: CC BY
Accession Number: edsair.doi.dedup.....59ed950d54ab75eec8e688f6944466dc
Database: OpenAIRE
Description
Abstract:The objective of this study is to analyze protein concentration on a colorimetric basis by utilizing the openly accessible 'ImageJ' software. We herein report the successful measurement of protein concentration on a parafilm surface, following the application of Bradford reagent and subsequent photography with a smartphone, followed by analysis using the ImageJ software. Ovalbumin (OVA) and bovine serum albumin (BSA) were dissolved in phosphate buffered saline (PBS) and used as standards. The results demonstrated a linear correlation between integrated density and the actual weight of the standard, with R² values of 0.9908 for OVA samples in the range of 0-1.5 mg/ml and 0.9781 for BSA in the range of 0.25-1.5 mg/ml. The straightforward nature of the experiments and analyses makes the study we present here an invaluable resource for researchers and students who are constrained by equipment limitations, such as the lack of access to spectrophotometers.
ISSN:25813250
DOI:10.30574/gscbps.2024.29.2.0426