Polyketide synthase positive Escherichia coli one‐time measurement in stool is not informative of colorectal cancer risk in a screening setting
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| Názov: | Polyketide synthase positive Escherichia coli one‐time measurement in stool is not informative of colorectal cancer risk in a screening setting |
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| Autori: | Willemijn de Klaver, Meike de Wit, Anne Bolijn, Marianne Tijssen, Pien Delis‐van Diemen, Margriet Lemmens, Manon CW Spaander, Evelien Dekker, Monique E van Leerdam, Veerle MH Coupé, Ruben van Boxtel, Hans Clevers, Beatriz Carvalho, Gerrit A Meijer |
| Prispievatelia: | CMM, Cancer, Hubrecht Institute with UMC |
| Zdroj: | de Klaver, W, de Wit, M, Bolijn, A, Tijssen, M, Delis-van Diemen, P, Lemmens, M, Spaander, M C W, Dekker, E, van Leerdam, M E, Coupé, V M H, van Boxtel, R, Clevers, H, Carvalho, B & Meijer, G A 2024, 'Polyketide synthase positive Escherichia coli one-time measurement in stool is not informative of colorectal cancer risk in a screening setting', Journal of Pathology, vol. 263, no. 2, pp. 217-225. https://doi.org/10.1002/path.6276 |
| Informácie o vydavateľovi: | Wiley, 2024. |
| Rok vydania: | 2024 |
| Predmety: | Male, Adenoma, Feces/microbiology, colorectal cancer, risk stratification, Risk Assessment, Pathology and Forensic Medicine, Feces, SDG 3 - Good Health and Well-being, Risk Factors, Escherichia coli, Biomarkers, Tumor, Adenoma/microbiology, Humans, Escherichia coli/isolation & purification, pks+ E. coli, Early Detection of Cancer, Aged, Tumor, screening, Colorectal Neoplasms/microbiology, Colonoscopy, Middle Aged, 16. Peace & justice, pks plus E. coli, 3. Good health, Polyketide Synthases/genetics, Case-Control Studies, advanced neoplasia, biomarker, Female, Early Detection of Cancer/methods, Colorectal Neoplasms, Polyketide Synthases, Biomarkers |
| Popis: | Environmental factors like the pathogenicity island polyketide synthase positive (pks+) Escherichia coli (E. coli) could have potential for risk stratification in colorectal cancer (CRC) screening. The association between pks+ E. coli measured in fecal immunochemical test (FIT) samples and the detection of advanced neoplasia (AN) at colonoscopy was investigated. Biobanked FIT samples were analyzed for both presence of E. coli and pks+ E. coli and correlated with colonoscopy findings; 5020 CRC screening participants were included. Controls were participants in which no relevant lesion was detected because of FIT‐negative results (cut‐off ≥15 μg Hb/g feces), a negative colonoscopy, or a colonoscopy during which only a nonadvanced polyp was detected. Cases were participants with AN [CRC, advanced adenoma (AA), or advanced serrated polyp (ASP)]. Existing DNA isolation and quantitative polymerase chain reaction (qPCR) procedures were used for the detection of E. coli and pks+ E. coli in stool. A total of 4542 (90.2%) individuals were E. coli positive, and 1322 (26.2%) were pks+ E. coli positive. The prevalence of E. coli in FIT samples from individuals with AN was 92.9% compared to 89.7% in FIT samples of controls (p = 0.010). The prevalence of pks+ E. coli in FIT samples from individuals with AN (28.6%) and controls (25.9%) was not significantly different (p = 0.13). The prevalences of pks+ E. coli in FIT samples from individuals with CRC, AA, or ASP were 29.6%, 28.3%, and 32.1%, respectively. In conclusion, the prevalence of pks+ E. coli in a screening population was 26.2% and did not differ significantly between individuals with AN and controls. These findings disqualify the straightforward option of using a snapshot measurement of pks+ E. coli in FIT samples as a stratification biomarker for CRC risk. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.6276 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/38551073 https://pure.eur.nl/en/publications/db7ba4ff-cd69-41ba-aa72-15d2896ec759 https://doi.org/10.1002/path.6276 https://pure.knaw.nl/portal/en/publications/c50f2f01-879c-4467-bc97-f5016320df6d https://hdl.handle.net/20.500.11755/c50f2f01-879c-4467-bc97-f5016320df6d https://doi.org/10.1002/path.6276 https://research.vumc.nl/en/publications/4588b10a-9b26-42ae-a8ae-69c13fdb6ed7 https://dspace.library.uu.nl/handle/1874/453295 https://pure.amsterdamumc.nl/en/publications/637ef792-ea17-41d1-9191-12629506255c https://doi.org/10.1002/path.6276 https://hdl.handle.net/1887/3764519 |
| Rights: | CC BY NC ND CC BY |
| Prístupové číslo: | edsair.doi.dedup.....59966ec3963e88424783a5cba8938175 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Environmental factors like the pathogenicity island polyketide synthase positive (pks+) Escherichia coli (E. coli) could have potential for risk stratification in colorectal cancer (CRC) screening. The association between pks+ E. coli measured in fecal immunochemical test (FIT) samples and the detection of advanced neoplasia (AN) at colonoscopy was investigated. Biobanked FIT samples were analyzed for both presence of E. coli and pks+ E. coli and correlated with colonoscopy findings; 5020 CRC screening participants were included. Controls were participants in which no relevant lesion was detected because of FIT‐negative results (cut‐off ≥15 μg Hb/g feces), a negative colonoscopy, or a colonoscopy during which only a nonadvanced polyp was detected. Cases were participants with AN [CRC, advanced adenoma (AA), or advanced serrated polyp (ASP)]. Existing DNA isolation and quantitative polymerase chain reaction (qPCR) procedures were used for the detection of E. coli and pks+ E. coli in stool. A total of 4542 (90.2%) individuals were E. coli positive, and 1322 (26.2%) were pks+ E. coli positive. The prevalence of E. coli in FIT samples from individuals with AN was 92.9% compared to 89.7% in FIT samples of controls (p = 0.010). The prevalence of pks+ E. coli in FIT samples from individuals with AN (28.6%) and controls (25.9%) was not significantly different (p = 0.13). The prevalences of pks+ E. coli in FIT samples from individuals with CRC, AA, or ASP were 29.6%, 28.3%, and 32.1%, respectively. In conclusion, the prevalence of pks+ E. coli in a screening population was 26.2% and did not differ significantly between individuals with AN and controls. These findings disqualify the straightforward option of using a snapshot measurement of pks+ E. coli in FIT samples as a stratification biomarker for CRC risk. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. |
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| ISSN: | 10969896 00223417 |
| DOI: | 10.1002/path.6276 |